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Detoxification method combining heat treatment and chemical treatment for apple tissue culture seedlings

A technology of chemical treatment and tissue culture seedlings, applied in botany equipment and methods, horticultural methods, horticulture, etc., can solve the problems of prolonged treatment time, plant phytotoxicity, and difficult operation, and achieve shortened time, high survival rate, and death low rate effect

Inactive Publication Date: 2015-03-25
FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

These methods all have weak point, adopt the method for stem tip culture, the stem tip size of cutting is generally 0.1-0.5mm, difficult operation, and survival rate is low; The survival rate is inversely proportional to the temperature, and the detoxification rate is directly proportional to the temperature. Taking into account both the survival rate and the detoxification rate, it is necessary to prolong the treatment time; It is directly proportional to the concentration, and when its concentration is higher than 25μg / mL, it will cause serious phytotoxicity to plants
At present, there has been no apple virus removal method that can guarantee the survival rate and obtain a good virus removal effect.

Method used

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  • Detoxification method combining heat treatment and chemical treatment for apple tissue culture seedlings
  • Detoxification method combining heat treatment and chemical treatment for apple tissue culture seedlings
  • Detoxification method combining heat treatment and chemical treatment for apple tissue culture seedlings

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Experimental program
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Embodiment 1

[0018] Embodiment 1, the detoxification method of apple tissue culture seedling heat treatment in conjunction with chemical treatment:

[0019] 1. Preparation of Ribavirin-containing medium:

[0020] Prepare the propagation medium of apple, the formula is MS+1mg / L 6-benzylaminopurine (6-BA)+0.1mg / L naphthaleneacetic acid (NAA), high temperature sterilization, when cooling to 60-70 ℃, in the absence of In the environment of bacteria, filter-sterilized viroethers were added to the culture medium and shaken well to make the final concentrations of 15 and 25 μg / mL respectively.

[0021] 2. Detoxification treatment:

[0022] When the apple tissue culture seedlings have multiplied to the required number, select the plants with the same growth, cut off the 0.7cm shoot tips, and transfer the shoot tips into the medium containing 15 and 25 μg / mL viridin respectively, and first place them at room temperature at 25°C. Place it for 1 day, then move it into the light incubator, and raise...

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Abstract

The invention discloses a method for removing apple viruses. According to the method, stem tips of apple tissue culture seedlings are inoculated into a culture medium containing 15 micrograms / milliliter-25 micrograms / milliliter of virus ether; and heating to 34-36 DEG C and carrying out constant-temperature heat treatment. The method has the advantages that the survival rate of plants is high, the detoxification effect is good, and the needed time is short; and a selected chemical agent has no host selectivity, so that the application prospect is wide.

Description

technical field [0001] The invention relates to a method for removing apple virus, in particular to a method for removing virus by combining heat treatment and chemical treatment of tissue cultured apple seedlings. Background technique [0002] Apples grown in my country are commonly infected with various viruses. Apple mosaic virus (ApMV), apple chlorotic leaf spot virus (ACLSV), apple stem pox virus (ASPV) and apple stem groove virus (ASGV) are the most common viruses infecting apples. After apples are infected with ApMV, bright yellow or yellow-white patches of different sizes will appear on the leaves, forming mosaic leaves, and there may also be reticulated symptoms of chlorosis and yellowing along the veins. ACLSV, ASGV and ASPV are latent in most apple cultivars and do not show symptoms, but only a few susceptible varieties or indicator plants show symptoms. In the main apple cultivation areas in my country, the carrying rate of the three latent viruses is as high a...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 胡国君董雅凤张尊平范旭东任芳周俊
Owner FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI
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