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Hair pretreatment method

A hair and hair cleaning technology, applied in the field of biochemistry, can solve problems such as complexity, narrow scope of application, low efficiency, etc., and achieve the effects of streamlining steps, not being easy to false positives, and reducing costs

Inactive Publication Date: 2015-03-18
HANGZHOU UMOTOR BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The present invention aims at the problems of narrow application range, complexity and low efficiency of the current hair pretreatment method, and proposes a hair pretreatment method

Method used

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Embodiment 1

[0022] The pretreatment method of embodiment 1 pig hair sample

[0023] The collected pig hairs were washed with 0.1% sodium lauryl sulfate and water respectively, dried at room temperature, and then cut into pieces of 2 mm. Weigh 10mg of pig hair into EP tube, add 500ul of digestive solution (PBS buffer containing 0.5% volume fraction, 1mmol / L calcium acetate, 5mmol / L sodium dodecyl sulfate, 10mmol / L dithiothreitol , 5mg / ml proteinase K, the balance is deionized water), 65 ℃ water bath, vortex every 10 minutes (can also be used in ultrasonic water bath to speed up the dissolution time) once, after 45 minutes all pig hairs are dissolved (30 minutes in ultrasonic minutes), the dissolution of pig hair is as follows figure 1 shown.

Embodiment 2

[0024] The pretreatment method of embodiment 2 people's hair sample

[0025] The collected human hair was washed with methanol, blotted dry with absorbent paper, and then cut into pieces of 2 mm. Weigh 2mg of hair into the EP tube, add 150ul of digestive solution (containing 0.1% volume fraction of PBS buffer solution, 1mmol / L calcium acetate, 5mmol / L sodium lauryl sulfate, 10mmol / L dithiothreitol, 15mg / ml proteinase K, the balance is deionized water), 65 ℃ water bath, vortex every 10 minutes (if possible, you can use water bath in ultrasonic to speed up the dissolution time) once, all hair will be dissolved after 35 minutes (25 minutes in ultrasonic ), hair dissolution as figure 2 shown.

Embodiment 3

[0026] Embodiment 3 detects the pretreatment method of clenbuterol in the pig hair sample

[0027] 3 pigs were found to be positive for clenbuterol in the on-site urine test screening at the slaughterhouse, and 10 negative pigs were selected immediately, the pig hairs of these 13 pigs were collected, and the hairs of these pigs were respectively treated with 0.1% dodecane Sodium sulfate and water, dried at room temperature, and then chopped into 2mm pieces. Weigh 10mg of pig hair into the EP tube, add 500ul of digestive solution (PBS buffer containing 0.1% volume fraction, 1mmol / L calcium acetate, 5mmol / L sodium lauryl sulfate, 5mmol / L dithiothreitol , 5mg / ml proteinase K, the balance is deionized water), in a water bath at 65°C for 20 minutes, vortexed every 5 minutes, and then placed in a water bath in boiling water for 5 minutes. detection. The clenbuterol detection kit was purchased from Hangzhou Dean Technology Co., Ltd., the trade name was clenbuterol one-step ELISA ki...

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Abstract

The invention discloses a hair pretreatment method belonging to the technical field of biological chemistry. The method comprises the following steps: cleaning hair by using a cleaning agent, and airing; crushing hairs into fragments, and finally, adding the hair fragments into digestive fluid, putting the digestive fluid into a water bath at the temperature of 60-100 DEG C for 5 minutes to 2 hours so as to hydrolyze the hairs, standing and cooling the digestive fluid to room temperature after the hydrolysis is finished, and detecting supernatant. According to the method disclosed by the invention, the hairs serve as a detection material, are easy to sample, non-traumatic and convenient to preserve and can be repeatedly sampled, the steps of a conventional hair pretreatment method are simplified and optimized, various components in the used digestive fluid do not influence with one another, the detection effect is good, the detection accuracy is high, the false positive condition is difficultly caused, and the misjudgment condition is avoided as much as possible in the detection process.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and in particular relates to a hair pretreatment method suitable for detecting drugs of abuse in hair. Background technique [0002] At present, the increasingly serious drug problem has become a global disaster. The proliferation of drugs directly endangers people's physical and mental health, and poses a huge threat to economic development and social progress. According to statistics from the United Nations, the annual drug trade volume in the world has reached more than 500 billion US dollars, and the spread of drugs has expanded to more than 200 countries and regions on five continents, and the number of people taking various drugs in the world has reached more than 200 million. Among them, young adults aged 17 to 35 accounted for 78%. On the other hand, in recent years, as countries around the world, especially developed countries such as the European Union, Japan, and the United Stat...

Claims

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Application Information

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IPC IPC(8): G01N1/28
Inventor 高颖魏建良
Owner HANGZHOU UMOTOR BIOTECH CO LTD
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