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Glucose oxidase mediation free radical initiating system and method for preparing hydrogel by using glucose oxidase mediation free radical initiating system

A technology of glucose oxidase and triggering system, applied in the field of polymer compounds, can solve the problems affecting the subsequent application of hydrogel, incomplete polymerization of monomers, poor storage stability, etc., achieve excellent mechanical properties and biocompatibility, and formulate Simple and effective in a wide pH range

Inactive Publication Date: 2015-03-18
TONGJI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, after the ferrous ions in the system are oxidized to ferric ions, it will also inhibit the chain growth process of monomer free radicals, resulting in incomplete polymerization of monomers, which will affect the subsequent application of the prepared hydrogel.
Moreover, due to the strong reducibility of ferrous ions, it is very easy to oxidize in the air, so the storage stability of ferrous sulfate as one of the initiating components is poor after being made into an aqueous solution, which will also affect the actual use.
The above-mentioned enzyme-mediated polymerization initiation system can prepare hydrogels in a short time (about 3 minutes), but there is no report on the controllability of the initiation gelation time.

Method used

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  • Glucose oxidase mediation free radical initiating system and method for preparing hydrogel by using glucose oxidase mediation free radical initiating system

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1) Preparation of precursor solution: Take 0.10~0.13g of N,N-dimethylacrylamide, crosslinking agent polyethylene glycol diacrylate (average molecular weight 250) 0.07~0.09g, and 1.3~1.5g deionized water into the sample In the bottle, mix evenly with a vortex mixer, and the measured pH value is 7-8.

[0030] 2) Preparation of hydrogel: add 100μL of N-hydroxysuccinimide aqueous solution, 100μL of glucose oxidase concentrate, and 200μL of glucose aqueous solution to the above precursor solution (N-hydroxysuccinimide and glucose in the reaction system). The molar concentration ratio is 1.74, and the molar concentration ratio of glucose and glucose oxidase is 25000), mix quickly, and let it stand tightly to obtain a light white translucent hydrogel. The gelation time is 3min30s.

Embodiment 2

[0032] 1) Preparation of precursor solution: take 0.10~0.13g of N,N-dimethylacrylamide, crosslinking agent polyethylene glycol diacrylate (average molecular weight 250) 0.07~0.09g, deionized water 1.3~1.5g, 1M Add 50 μL of the acetic acid aqueous solution into the sample bottle and mix evenly with a vortex mixer. The measured pH value is 3.5-4.5.

[0033] 2) Preparation of hydrogel: The procedure is the same as in Example 1, and the gel time is 3 minutes.

Embodiment 3

[0035] 1) Preparation of precursor solution: take 0.10~0.13g of N,N-dimethylacrylamide, crosslinking agent polyethylene glycol diacrylate (average molecular weight 250) 0.07~0.09g, deionized water 1.3~1.5g, 1M Add 125 μL of ammonia solution into the sample bottle and mix evenly with a vortex mixer. The measured pH value is 9.5 to 10.5.

[0036] 2) Preparation of hydrogel: The procedure is the same as in Example 1, and the gel forming time is 19 minutes and 30 seconds.

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Abstract

The invention relates to a glucose oxidase mediation free radical initiating system and a method for preparing hydrogel by using the glucose oxidase mediation free radical initiating system. The glucose oxidase mediation free radical initiating system consists of a N-hydroxyphthalimide derivative, glucose oxidase and glucose and has the advantages that the initiation condition is gentle, the preparation is simple and convenient, the pH value application range of a reaction medium is relatively wide, and the environment can be protected. According to the method provided by the invention, by adjusting the pH value of the reaction medium or adjusting the concentration ratio of components of a ternary initiating system, monomer polymerization can be initiated within 3-30 minutes at the room temperature under control to prepare hydrogel, and high-strength nano composite hydrogel can be also prepared, so that the method has remarkable application prospect in fields such as controlled-release of medicines, enzyme immobilization, tissue engineering and substance separation.

Description

Technical field [0001] The invention relates to the field of polymer compounds, in particular to a novel glucose oxidase-mediated free radical polymerization initiation system and a method for preparing hydrogels. Background technique [0002] Polymer compounds play an important role in the field of materials and are widely used in industries, agriculture, biomedicine, environmental protection, and civil life. Common polymer synthesis reactions usually need to control temperature and pressure, which requires high reaction equipment, and sometimes requires the use of toxic catalysts. With the increasing awareness of the sustainable development of human society, the use of high-efficiency, non-toxic, and environmentally friendly synthetic methods to prepare required polymer products is the direction of polymer synthetic chemistry research. [0003] Biological enzyme is a kind of protein with catalytic function and adjustable activity produced by living cells. It has the following ch...

Claims

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Application Information

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IPC IPC(8): C08F220/54C08F222/14C08F120/54C08F290/02C08F226/10C08F4/40C08K3/34
Inventor 苏腾王启刚唐舟李汶军何鸿舰
Owner TONGJI UNIV
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