Method for preparing mannan-oligosaccharide through enzymolysis of fine konjak powder

Abstract

The invention provides a method for preparing mannan-oligosaccharide from fine konjak powder through the combination of an enzymic method and an ultra-filtration process. The mannan-oligosaccharide is obtained by performing enzymolysis on the fine konjak powder by utilizing recombination beta-mannase (reAuMan26A). A key enzyme used in the method has higher catalytic activity; the production and use cost of the enzyme is lower; the production period is short; pollution is avoided; purifying and separating steps are simple and convenient; the additional value of the fine konjak powder is greatly increased; a theoretical basis is laid for further refining the mannan-oligosaccharide; the greater industrial production and application potential, and a greater economic value are achieved.

Description

technical field [0001] The invention relates to a method for preparing mannose oligosaccharides by hydrolyzing konjac powder with biological enzymes, and belongs to the technical field of bioengineering. Background technique [0002] Mannooligosaccharides (Mannooligosaccharides, MOS) are oligosaccharides formed by 2 to 10 monosaccharide molecules linked by β-1,4 or β-1,3 glycosidic bonds. It is produced by hydrolyzing mannan (extracted from plants such as konjac, carob gum and guar gum) with β-mannanase produced by bacteria such as Bacillus and Bacillus licheniformis. Studies have shown that mannose oligosaccharides can effectively promote the specific proliferation of beneficial intestinal flora such as Bifidobacterium and Lactobacillus, reduce intestinal pH to inhibit the growth of harmful bacteria; Non-specific immunity, promoting the secretion of mannose-binding protein in the liver to enhance the body's immunity; it also has the characteristics of improving serum lipid...

AI Technical Summary

Problems solved by technology

At present, mannose oligosaccharides are usually prepared by high-temperature hydrolysis, acid hydrolysis or alkaline hydrolysis. Although enzymatic hydrolysis has also been reported, the conditions are not mature enough.

The enzymatic production of mannose oligosaccharides is mainly limited by the low catalytic efficiency of the key enzymes used in the production, the high cost of enzyme production and use, and the high viscosity of the substrate, which makes it difficult to achieve high-concentration production, etc.

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