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Method for the determination of ochratoxin a in fruit juice

A technology of ochratoxin and fruit juice, which is applied in the direction of material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of poor feasibility, poor determination selectivity, difficulty in meeting analysis requirements, etc., and achieve the effect of high sensitivity and simple method

Inactive Publication Date: 2017-11-17
CHINA AGRI UNIV
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Problems solved by technology

However, the total emission fluorescence value is measured, and the selectivity for single substance determination in complex matrices is poor. Since the fluorescence spectrum of ochratoxin A in fruit juice mixes and overlaps with the spectrum of complex sample matrices, this method can be directly used to detect ochratoxin in it without separation. The feasibility of toxin A content is poor, that is, the conventional fluorescence analysis method is difficult to meet the analysis requirements

Method used

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  • Method for the determination of ochratoxin a in fruit juice
  • Method for the determination of ochratoxin a in fruit juice
  • Method for the determination of ochratoxin a in fruit juice

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Experimental program
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Embodiment

[0055] 1. Operation steps

[0056] Step (1), based on the parallel factor method (PARAFAC), establish a calibration model for quantitative analysis based on the standard

[0057] 1) Preparation of standard products:

[0058] Take 1 mg of ochratoxin A standard substance and dissolve it completely in chromatographic grade methanol, dilute to 50 mL (20 μg / mL, store in the dark at -20°C), and use it as ochratoxin A standard stock solution.

[0059] Take 0.5mL ochratoxin A standard stock solution, dilute to 100mL with methanol, the concentration is 100ng / mL (stored in the dark at 4°C) for later use; use dilute sodium bicarbonate solution to prepare a series of ochratoxin A solutions with different concentrations , scan the 3D fluorescence intensity of the samples one by one, and conduct a linear range inspection. The correlation coefficient is 0.99 within the concentration range of 0.27-3.24ng / mL, and the linear relationship is good for quantitative analysis.

[0060] 2) Preparat...

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Abstract

The invention discloses a method for determining the content of ochratoxin A in fruit juice. The samples were pretreated by a simple liquid-liquid extraction purification step, and then scanned and collected the three-dimensional fluorescence data of the standard and samples under the optimized scanning wavelength and scanning interval parameters, and the obtained data were analyzed by parallel factor analysis (PARAFAC) Carry out mathematical separation processing, use "mathematical separation" to combine chemical and physical separation, use known concentration standards to establish a calibration model, and realize the prediction of the components to be measured in the case of unknown, uncorrected background interference and serious spectral overlap . The method is simple, rapid, and highly sensitive, and can realize the determination of ochratoxin content in fruit juice under the interference of unknown background. It belongs to the field of food safety.

Description

technical field [0001] The invention relates to a method for the content of ochratoxin A, in particular to a method for measuring the content of ochratoxin A in fruit juice based on a three-dimensional fluorescence second-order correction method, and belongs to the field of food safety. technical background [0002] Ochratoxin A (OTA) is a toxic metabolite produced by fungi, commonly found in grains and their products, coffee, fruits and their products. The International Agency for Research on Cancer (IARC) has identified it as a 2B carcinogen. At present, there is no clear limit standard for ochratoxin A in fruit juice in my country, especially its rapid analysis and detection methods are less studied. [0003] The main detection methods of ochratoxin A include thin-layer chromatography, enzyme-linked immunoassay, immunoaffinity column chromatography purification fluorescence photometry, solid-phase extraction high-performance liquid chromatography, liquid-liquid extraction...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 王军冯清清林亚青陈敏
Owner CHINA AGRI UNIV
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