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Molecular marking method for indicating common premunition of sheep

A technology of molecular markers and disease resistance, applied in the field of animal molecular genetics, can solve the problems of high price, low result accuracy, and low cost, and achieve the effects of increasing sheep survival rate, reducing morbidity, and low cost

Active Publication Date: 2015-02-18
XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

DNA sequencing is relatively accurate and expensive
High-resolution melting curve method (HRM) is a SNP research tool that has emerged in recent years. This method does not need to design probes, and the cost is low, but the accuracy of the results is low.

Method used

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  • Molecular marking method for indicating common premunition of sheep
  • Molecular marking method for indicating common premunition of sheep
  • Molecular marking method for indicating common premunition of sheep

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0031] Embodiment: the following examples describe the present invention in more detail:

[0032] 1. Detection and analysis of the mutation site in exon 9 of sheep IDO1 gene

[0033] 1. Sample collection

[0034] Draw about 1 mL of blood from the jugular vein of sheep with a disposable syringe, inject it into a 1.5 mL centrifuge tube that has been autoclaved and filled with about 200 μL of 2% sterile EDTA (Ethylene diamine tetraacetic acid, EDTA) anticoagulant, and shake gently , record the number of sheep, and store at -20°C for later use.

[0035] 2. Drugs and enzymes

[0036] Tris (Tris), Sigma Chemicals Co; Tris saturated phenol, EDTA-Na 2 Beijing Dingguo Biotechnology Development Center; Proteinase K (Proteinase K), MERCK Co; dNTP (dATP; dTTP; dCTP; dGTP), Taq enzyme (supporting 10×Taq buffer, 25mmol / L Mg 2+ ), Dalian Bao Biological Company; agarose (Agarose H), pUC19DNA / Msp (Hpa Ⅱ) Marker, Sangon Bioengineering (Shanghai) Co., Ltd.; restriction endonuclease Nhe Ⅰ, Fe...

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Abstract

The invention relates to the field of animal molecular genetics, and relates to the technology of genetics and breeding and marker-assisted selection of sheep. A molecular marking method for indicating common premunition of sheep comprises the following steps: designing a pair of primers according to the indoleamine 2,3-dioxygenase 1 (IDO1) gene sequence of sheep; extracting genomic DNAs (deoxyribonucleic acids) from the blood of the sheep, and utilizing the primer INF and the primer INR to carry out PCR (Polymerase Chain Reaction) amplification on the genomic DNAs of the sheep; using restriction enzyme Nhe I for digestion by PCR amplification products, carrying out electrophoretic separation on digestion products through sepharose gel, and carrying out genotype decision according to the result of the electrophoretic separation; constructing a genotype effect statistic model; dividing the group of the sheep into three types according to genotype, and selecting AA genotype individuals for grouping so as to complete molecular marking for the common premonition of sheep. The molecular marking method for indicating the common premonition of sheep is convenient in operation, economic and practical, and is more effective and accurate, so that the accuracy of strain selection can be improved.

Description

technical field [0001] The invention belongs to the field of animal molecular genetics, belongs to sheep genetic breeding and molecular marker-assisted selection technology, specifically relates to the screening and application of genetic markers for general disease resistance of sheep, and particularly relates to a molecular marker method for predicting general disease resistance of sheep. Background technique [0002] At present, the main purpose of sheep breeding is to fully improve specific production performance such as meat and wool according to specific breeding goals, so as to obtain the greatest production economic benefits. With the popularization of intensive feeding methods and the improvement of the degree of breeding, the selection pressure on important economic traits of sheep has increased, and the disease resistance of sheep has decreased, resulting in an increase in the incidence of diseases, especially infectious diseases. At present, the control measures ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/683C12Q1/6888C12Q2600/124C12Q2600/156C12Q2531/113C12Q2521/301C12Q2565/125
Inventor 杨华杨永林钟发刚赵文娟康立超
Owner XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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