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Biological sensor, manufacturing method and detection method thereof

A technology of biosensor and manufacturing method, which is applied in the field of flexible full-print nano-carbon material biosensor, can solve the problems of complex processing technology, difficult mass production, and large signal noise, and achieve high sensitivity, good stability, and reliable connection. Effect

Active Publication Date: 2015-02-11
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the attempts in the field of biosensors are still in their infancy, the signal noise is large, the detection limit is high, the processing technology is complicated, and it is difficult to mass produce

Method used

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  • Biological sensor, manufacturing method and detection method thereof
  • Biological sensor, manufacturing method and detection method thereof
  • Biological sensor, manufacturing method and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] ginseng figure 1 As shown, the biosensor of this embodiment includes: a flexible substrate 1 , and a source 2 a and a drain 2 b arranged in parallel on the flexible substrate 1 .

[0044] Both the source electrode 2 a and the drain electrode 2 b include a parallel portion 21 and an extension portion 22 for electrically connecting with an external circuit (not shown in the figure).

[0045] The biosensor also includes a functionalized graphene layer 3 arranged between the source electrode 2a and the drain electrode 2b, and the functionalized graphene layer 3 is respectively connected to the parallel portion 21 of the source electrode 2a and the parallel portion 21 of the drain electrode 2b; the functionalized graphene layer The surface of layer 3 is modified with specific receptors.

[0046] The biosensor also includes a floating gate 4 , one end of which is suspended above the source 2 a and the drain 2 b without contacting the graphene layer 3 .

[0047] Below, combine...

Embodiment 2

[0062] The structure of the flexible all-printed biosensor of this embodiment is the same as that of Embodiment 1. The difference is that the flexible substrate is polyethylene terephthalate PET, the source-drain distance is 200um, the silver paste concentration is 0.08mg / mL, and the nano-carbon material used is 0.05mg / mL single-walled carbon nanotubes, called The mass of 1-pyrenebutyric acid was 120 mg, and the blocking reagent was 5% bovine serum albumin BSA.

[0063] Moreover, in this embodiment, a solution of functionalized single-walled carbon nanotubes with a concentration of 0.05 mg / mL is printed between the source electrode and the drain electrode by means of aerosol printing. Thereafter, the chip was placed in a mixed solution of 4 mM (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) EDC and 10 mM N-hydroxysuccinimide NHS for 1 h, and activated graphite Functionalized carboxyl groups adsorbed on alkenes.

[0064] The remaining steps can be shown in Examp...

Embodiment 3

[0066] The structure of the graphene ion-sensitive sensor of this embodiment is the same as that of Embodiment 1. The difference is polyethylene PE, the distance between source and drain is 500um, the concentration of silver paste is 0.06mg / mL, the nano-carbon material used is 0.02mg / mL single-walled carbon nanotubes, and the mass of 1-pyrene butyric acid is weighed as 100mg. The blocking reagent was 2.5% skimmed milk.

[0067] Moreover, in this embodiment, a solution of functionalized single-walled carbon nanotubes with a concentration of 0.02 mg / mL is printed between the source electrode and the drain electrode by means of aerosol printing. Thereafter, the chip was placed in a mixed solution of 2mM (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) EDC and 5mM N-hydroxysuccinimide NHS for 3h, and activated graphite Functionalized carboxyl groups adsorbed on alkenes.

[0068] The remaining steps can be shown in Example 1 with reference to.

[0069] In other embo...

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Abstract

The invention discloses a biological sensor and a manufacturing method and a detection method thereof. The biosensor has a field effect transistor structure, which comprises a flexible substrate, printed silver source drain electrode arranged on the flexible substrate and a printing functionalized nano carbon material layer connected with the source drain electrode. A specific receptor is connected with the functionalized nano carbon material layer as a sensitive material for detecting minute quantity of a specific target material. The source drain electrode is connected with a set of signal detecting system, which cooperates with signal regulation of a suspended grid in a detection liquid to realize the rapid, accurate and sensitive biological diagnosis; therefore, the biological sensor has the advantages of integration, compatibility and portability.

Description

Technical field [0001] The present invention involves a technical field and its preparation methods of a biosensor, and specially involves a flexible full -facing nano -carbon material biosensor and their production methods and detection methods based on a field -effect transistor structure. Background technique [0002] Graphene is a new material made of a single -layer structure composed of carbon atoms. It is a carbon quality material that combines semiconductors and metal properties. It has excellent performance such as heat, force, and electricity.Graphene is a zero -band semiconductor, with unique load characteristics and excellent electrical properties.Carbon nanotubes are a unique carbon nanoprobine structure that can be regarded as a large layer or multi -layer carbon atom curling in the graphite.Carbon nanotubes have many unique properties, such as good mechanical properties, high thermal stability, excellent conductivity and thermal conductivity.These nano -carbon mate...

Claims

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Application Information

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IPC IPC(8): G01N27/414G01N33/551
Inventor 张珽于薇蔡培杰刘瑞
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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