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Preparation method for chlorella antibacterial peptide

A technology of chlorella and bacteriostatic peptides, which is applied in the field of preparation of chlorella bacteriostatic peptides, can solve the problems of lack of depth, etc., and achieve the effects of high purity, easy availability of raw materials, low cost, and mild process conditions

Active Publication Date: 2015-02-11
安徽汉芳生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In 2007, Wang Qin et al. conducted antibacterial experiments on algae powder extracts of 22 kinds of microalgae, and found that different extraction solvents had certain effects on antibacterial activity, but the extracts had no inhibitory effect on the growth of Gram-positive and negative bacteria. obvious difference
However, compared with foreign research, the current domestic work is still lacking in depth, and there are some problems that need to be solved urgently

Method used

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  • Preparation method for chlorella antibacterial peptide
  • Preparation method for chlorella antibacterial peptide
  • Preparation method for chlorella antibacterial peptide

Examples

Experimental program
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Effect test

Embodiment 1

[0029] 1. Optimization of Extraction Process of Chlorella Protein

[0030] 1.1 Materials and reagents

[0031] Chlorella dry powder, from Zhejiang Hangzhou Huadan Agricultural Products Co., Ltd.; Bio-Rad Protein Assay kit, purchased from Bio-Rad Laboratories Inc; copper sulfate, potassium sulfate, sulfuric acid, boric acid, methyl red indicator (C 15 h 15 N 3 o 2 ), bromocresol green indicator (C 21 h 14 Br 4 o 5 S), methylene blue indicator (C 16 h 18 ClN 3 S·3H 2 O), sodium hydroxide, 95% ethanol, boric acid solution (20g / L), mixed indicator solution (2 parts of methyl red ethanol solution and 1 part of methylene blue ethanol solution are mixed immediately before use).

[0032] 1.2 Experimental Instruments

[0033] Enzyme-linked immunosorbent detection instrument (Model-550), American Bio-Rad Company; JN-02C low-temperature ultra-high pressure continuous flow cell crusher, Guangzhou Juneng Biotechnology Co., Ltd.; high-speed refrigerated centrifuge (5415R), Germ...

Embodiment 2

[0103] (1) Pretreatment of chlorella protein: Take 40g of chlorella powder, add 200mL of water and mix well, freeze at -40°C for 2 hours, melt at room temperature, add 1200mL of water (total material-to-liquid ratio is 1:35) , placed in an ultrasonic extractor, ultrasonically treated at 30°C and 20KHz for 20 minutes, then treated with a low-temperature ultra-high pressure continuous flow cell disruptor for 8 minutes at a pressure of 200Mpa, ultrasonically extracted at 55°C and 20KHz for 130 minutes, and centrifuged ( 10000r / min, centrifuged for 20min), to obtain supernatant 1 and filter residue 1;

[0104] (2) The filter residue 1 was extracted 3 times according to the condition of step (1), and the supernatants 2 to 4 were obtained in turn, and the supernatants 1 to 4 were combined to obtain the chlorella extract; calculated according to the method of Example 1, the chlorella The extraction rate of protein is 62%;

[0105] (3) Combine the three extracts of chlorella after ul...

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Abstract

The invention provides a preparation method for a chlorella antibacterial peptide. According to the invention, chlorella is adopted as the raw material, ultralow temperature freezing and low temperature ultrahigh pressure continuous flow cell disruption are combined with an ultrasonic treatment process, effective wall-breaking is carried out on chlorella to obtain soluble protein, and then a biological enzyme method is utilized to obtain the antibacterial peptide with obvious antibacterial effect. The chlorella has the characteristics of extremely solid cell wall and high intramural protein content, and the technical key points of the invention lie in that ultralow temperature freezing and low temperature ultrahigh pressure continuous flow cell disruption are combined with the ultrasonic treatment process to treat chlorella, effective wall-breaking on chlorella is realized, and the content of soluble protein is increased. Through selection of appropriate enzyme and control of hydrolysis degree, the antibacterial peptide with a good antibacterial effect can be obtained.

Description

(1) Technical field [0001] The invention relates to a preparation method of chlorella bacteriostatic peptide. (2) Background technology [0002] Foreign scholars began to study marine antibacterial active substances in the 1940s. 50 years ago, they discovered and successfully developed the first marine new antibiotic - cephalosporin, which pioneered the development of new marine antibiotics. the first of its kind. Since Pratt first isolated the chlorellin fatty acid mixture with antibacterial activity from Chlorella in 1944, the scientific research on extracting substances that kill human pathogenic bacteria from algae has been deepening. In 1962, Starr et al. found that the methanol extract of the Hawaiian cyanobacterium Lyngbya majuscula had antibacterial activity. Since then, Susann has studied different cyanobacteria and found that they can produce a variety of antibacterial substances, which can be used as an important source of antibiotics. In 1988, Murakami isolate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 张拥军张高帆唐王裔孙佳悦唐旋陈丽莎朱丽云
Owner 安徽汉芳生物科技有限公司
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