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Application of xerophilusin B in preparation of products for inhibiting tumor growth

A tumor and product technology, applied in anti-tumor drugs, medical preparations containing active ingredients, organic active ingredients, etc., can solve problems such as no literature reports on anti-esophageal cancer activity

Active Publication Date: 2015-02-11
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there is no literature report on the anti-esophageal cancer activity of ent-kaurene diterpene xerophilusin B

Method used

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  • Application of xerophilusin B in preparation of products for inhibiting tumor growth
  • Application of xerophilusin B in preparation of products for inhibiting tumor growth
  • Application of xerophilusin B in preparation of products for inhibiting tumor growth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Embodiment 1, the acquisition of compound Xerophilusin B (abbreviated as spxl-3)

[0087] 1) Extraction and purification

[0088] a) Grind and dry the leaf part of the collected xeric tea plant as plant raw material (7.5 kg), pulverize it at room temperature and soak it in an acetone solution with a volume of 12 L, and replace it with fresh acetone every 3 days solution.

[0089] b) filtering the soaked plant material at room temperature, and collecting the filtrate. The collected filtrate was distilled under reduced pressure to remove the solvent to obtain a crude extract.

[0090] c) Dissolve the crude extract in b) in water (4L), extract and extract with petroleum ether (3 times in total, 3L each time) and ethyl acetate (3 times in total, 4L each time), and collect the concentrate Promptly obtain ethyl acetate extract extract (504 grams).

[0091] d) decolorize the ethyl acetate extract (504 g) using MCl resin, and use 90% MeOH-H 2 Elution of O yielded a pale ye...

Embodiment 2

[0100] Example 2, the application of Xerophilusin B in inhibiting the growth of esophageal squamous cell carcinoma

[0101] 1. Inhibitory effect of Xerophilusin B on the growth of esophageal squamous cell carcinoma cell lines in vitro

[0102] Determination of the cell growth inhibition rate experimental reagent adopts the Cell Counting Kit-8 (CCK-8) kit of Dojindo Company, and the specific method is as follows:

[0103] 1) Culture and expand KYSE-140, KYSE-150, KYSE-450, KYSE-510, NIH-3T3 and HEK-293 cells until the cell confluence reaches 70-80%, using 0.25% trypsin Digest and process the cells, then pipette with modified RPMI-1640, DMEM or MEM complete medium to make a uniform cell suspension, and place it in a 15ml or 50ml centrifuge tube.

[0104] 2) Dilute the resuspended cell suspension by adding complete medium to make the final concentration reach 1.5×10 4 cells / mL, after thorough mixing, pipette 200 μL into a 96-well plate (each well contains about 2000 cells), and...

Embodiment 3

[0213] Embodiment 3, preparation of anti-esophageal squamous cell carcinoma drug

[0214] According to the above-mentioned Example 2, it can be seen that Xerophilusin B can be used as an active ingredient for the treatment of anti-esophageal squamous cell carcinoma drugs, and anti-esophageal squamous cell carcinoma drugs are prepared according to conventional pharmaceutical preparations, as follows:

[0215] Drug 1: Xerophilusin B, and preparations made by using mixed solvents, nano drug crystal technology and cyclodextrin, adding water for injection as usual, fine filtration, potting and sterilization to make injections.

[0216] Drug 2: Xerophilusin B, and preparations made using mixed solvents, nano drug crystal technology and cyclodextrin, dissolved in sterile water for injection, stirred to dissolve, filtered with a sterile suction filter funnel, and then sterile fine filtered , freeze-dried at low temperature and sealed aseptically to obtain a powder injection.

[0217]...

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Abstract

The invention discloses application of xerophilusin B in preparation of products for inhibiting tumor growth. The xerophilusin B can be applied to preparation of products with the following functions: (1) preventing or treating tumors and (2) inhibiting proliferation of tumor cells, wherein the chemical structural formula of the xerophilusin B is as shown in a formula I in the specification. Therefore, in consideration of constant discovery of various novel compounds in plectranthus plants and remarkable effects discovered in tumor treatment and researches, along with gradually further researches on compound ingredients extracted from plectranthus plants, diterpenoid compounds represented by the xerophilusin B can bring a novel approach for tumor treatment.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of xerophilusin B in the preparation of products for inhibiting tumor growth. Background technique [0002] Esophageal cancer is one of the malignant tumors with high incidence worldwide and a serious threat to human health and life. Currently, the incidence of esophageal malignant tumors ranks eighth among all malignant tumors. Different from esophageal adenocarcinoma in European and American countries, esophageal cancer in my country has always been dominated by squamous cell carcinoma, and the incidence of esophageal adenocarcinoma has not increased significantly. More than 90% of esophageal cancer cases are esophageal squamous cell carcinoma, so esophageal squamous cell carcinoma Carcinoma is a malignant tumor with Chinese characteristics. And in my country, esophageal cancer has obvious geographical distribution and aggregation phenomenon, and the areas with high...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P35/00
CPCA61K31/352
Inventor 赫捷孙汉董陈照丽普建新尧然汪伟光
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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