Method for measuring light stability of monascus red pigment
A technology of monascus red pigment and light stability, which is applied in the field of food additives and its detection, can solve the problems of long measurement time, meaningless, inconvenient operation, etc., and achieve the effect of high repeatability, high accuracy and easy operation
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[0013] First extract the commercially available powdery or pasty monascus pigment with chloroform:methanol=50:50, then pass through the silica gel column soaked in chloroform, and wash with chloroform:methanol=90:10 to remove the polarity Small free pigments were washed with chloroform:methanol=50:50, and the pigments obtained after separation were subjected to thin-layer chromatography on a silica gel plate. The chromatographic agent was chloroform:methanol:water=65:25:4. Then get the separated red, orange and yellow pigments, dissolve the obtained pigments in distilled water respectively to make a certain 0.01% pigment solution, and use distilled water as a blank, and use a UV-visible spectrophotometer at 490nm, 374nm, Measure the initial absorbance of red, orange and yellow pigment solutions at 367nm. Then take a certain amount of pigment solution and place it in a Φ12cm plate. The thickness of the pigment solution in the plate is 4.5mm. Put the plate under a 4OW UV lamp an...
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