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A brush-type hydrazine polymer functionalized magnetic nanomaterial and its preparation and application

A magnetic nano and polymer technology, applied in chemical instruments and methods, preparation of test samples, and other chemical processes, to achieve simple operation, reduce sample loss, and improve biocompatibility

Active Publication Date: 2016-07-27
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The brush-type hydrazine polymer functionalized magnetic nanomaterials were prepared by RAFT on the magnetic core and applied to the separation and enrichment of glycopeptides or glycoproteins. The method has not been reported.

Method used

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  • A brush-type hydrazine polymer functionalized magnetic nanomaterial and its preparation and application
  • A brush-type hydrazine polymer functionalized magnetic nanomaterial and its preparation and application
  • A brush-type hydrazine polymer functionalized magnetic nanomaterial and its preparation and application

Examples

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Embodiment Construction

[0032] Example Using brush-shaped hydrazine polymer to functionalize magnetic nanomaterials to enrich glycopeptides

[0033] Preparation of brush-shaped hydrazine polymer functionalized magnetic nanomaterials:

[0034] 1) 300μgFe 3 O 4 The particles were dispersed in 200mL ethanol, 50mL water and 1.4mL concentrated ammonia water, sonicated for 15min, then added 1mLTEOS, stirred at room temperature for 10h, the product was washed with 30mL water and ethanol 5 times each, and placed in a vacuum drying oven at 50℃ Dry for 24h to get Fe 3 O 4 SiO 2 Particles

[0035] 2) Add 200μgFe 3 O 4 SiO 2 Disperse the particles into 40mL of dry toluene. After sonicating for 15 minutes, drop 8mL of toluene solution containing 4-chloromethyltrichlorosilane (90μL). After sonicating for 5 minutes, slowly drop 8mL of toluene solution containing triethylamine (216μL). The final reaction solution was refluxed for 24 hours under the protection of nitrogen. The product was washed 5 times with 30 mL of water...

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Abstract

The invention relates to a brush-type hydrazine-polymer functionalized magnetic nano-material, preparation and application thereof to enriching of glycopeptide or glycoprotein. After a reversible addition-fragmentation chain transfer (RAFT) reagent is introduced into the surface of a nano-material, an organic polymerization reaction functional monomer and an organic polymerization reaction initiator are added, the organic functional monomer is subjected to controllable free-radical polymerization under the regulation and control of the reversible addition-fragmentation chain transfer reagent, so that the brush-type polymer organic-inorganic hybrid nano-material is prepared. The organic-inorganic hybrid nano-material is simple in preparation process and mild in reaction conditions, and is applicable to enriching of glycopeptide or glycoprotein in a complex biological sample.

Description

Technical field [0001] The invention relates to the separation and enrichment of glycosylated peptides, in particular to a brush-shaped hydrazine polymer functionalized magnetic nanomaterial and its preparation, and its application in the selective separation and enrichment of glycopeptides or glycoproteins. Background technique [0002] As an important post-translational modification, protein glycosylation has an important influence on protein folding, conformational stability and activity. Glycosylated proteins are involved in protein interaction, immune response, cell communication, cell apoptosis and other life processes. Abnormal changes in glycosylated proteins can be used as an important sign of tumor occurrence and development. [0003] In the analysis of glycosylated proteome, high performance liquid chromatography mass spectrometry is an effective tool for analyzing glycoproteins or glycopeptides. When the sample is directly analyzed by mass spectrometry, since the cont...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/286B01J20/30B01D15/08C07K1/14G01N1/34
CPCB01D15/08B01J20/286B01J20/3085B01J2220/445B01J2220/80C07K1/22G01N1/34
Inventor 邹汉法黄光欧俊杰
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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