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Streptococcus suis vaccine composition, and preparation method and application thereof

A vaccine composition, Streptococcus suis technology, applied in the field of veterinary biological products, can solve the problems of poor immunogenicity of capsular polysaccharides and unsatisfactory results, no vaccine Streptococcus suis infection, limitations of immune protection, etc. , to achieve the effect of preventing and/or treating Streptococcus suis infection, perfecting Streptococcus suis infection, and facilitating large-scale production

Active Publication Date: 2014-12-31
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Thirty-three serotypes (types 1-31, 33, and 1 / 2) based on capsular antigens are currently known, but the mechanisms involved in the pathogenesis and virulence of S. There is an effective vaccine to prevent and control Streptococcus suis infection
[0003] The existing inactivated whole-bacteria vaccines and live non-toxic vaccines require repeated immunizations and cannot resist the attack of heterogeneous strains, so their protective effects are very limited
Due to the important role of the S. suis capsule in pathogenicity, attempts were made to develop vaccines based on capsular material, however, such vaccines did not achieve satisfactory results due to the poor immunogenicity of the capsular polysaccharide
Attempts have been made to develop subunit vaccines using suilysin, or lysozyme-releasing protein (MRP) and extracellular protein factor (EF), however, in some geographic areas a significant number of highly virulent strains do not express These proteins, resulting in the limitations of its immune protection

Method used

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  • Streptococcus suis vaccine composition, and preparation method and application thereof
  • Streptococcus suis vaccine composition, and preparation method and application thereof
  • Streptococcus suis vaccine composition, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Preparation of Immunogenic Antigen of Streptococcus suis

[0046] S. suis immunogenic antigens can be prepared by various methods known to those skilled in the art, such as by cloning or expression vectors comprising polynucleotides of the present invention. . Publicly available vectors suitable for preparing S. suis polypeptide antigens of the invention include plasmids, adenoviruses, baculoviruses, yeast baculoviruses, plant viruses, adeno-associated viruses, retroviruses, herpes simplex virus, alphaviruses, lentiviruses, Viruses, etc., and methods for constructing such vectors are also available. The preparation of the Streptococcus suis polypeptide antigen in the invention also includes artificially synthesized polypeptide antigens. The embodiment of the present invention adopts E. coli expression system to prepare 38KDA protein and SaoA protein.

[0047] 1. Materials

[0048] The pMD18-T vector used in the present invention (purchased from Treasure Bioengineeri...

Embodiment 2

[0068] Preparation of Streptococcus suis vaccine composition

[0069] Take the 38KDA and SaoA proteins prepared in Example 1 and slowly add them to the adjuvant. During the process of adding, stir continuously for 12 minutes with an emulsifier with a rotation speed of 800 rpm, mix well, and store at 4°C. This is the vaccine composition for Streptococcus suis. See Table 2 for specific ratios. Adjuvants suitable for the present invention can be oil adjuvants well known to those skilled in the art, aqueous emulsions (for example, complete Freund's adjuvant and incomplete Freund's adjuvant), water-soluble adjuvants, Quil A adjuvant, hydrogen One or more of aluminum oxide adjuvant, dextran, dextran sulfate, and sodium alginate. In this embodiment, the water-soluble adjuvant gel adjuvant (Sepic company, France) is selected.

[0070] Table 2 Streptococcus suis vaccine composition ratio

[0071]

Embodiment 3

[0073] Immunogenicity test of Streptococcus suis vaccine composition

[0074] 1. Immunization procedure

[0075] Use 5-7 week-old clean level Kunming mice for immunogenicity test, divide into 8 groups according to test requirements, 1-7 groups are respectively vaccine 1, vaccine 2, vaccine 3, vaccine prepared by Example 2 of the present invention 4. Vaccine 5, vaccine 6, vaccine 7 immunization groups, group 8 PBS control group. The immune group was immunized by subcutaneous injection of 0.4ml on the back, and the PBS control group was immunized with the same amount of PBS, followed by booster immunization once 14 days later. The antibody levels of vaccine 1, vaccine 2, vaccine 3, vaccine 4, vaccine 5, vaccine 6, vaccine 7 and the PBS control group were detected 14 days and 28 days after the first immunization, respectively.

[0076] 2. Detection of antibody level

[0077] The mice in each group were blood collected 14 days and 28 days after the first immunization, and the i...

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Abstract

The invention relates to the field of biological products for animals, and specifically relates to a streptococcus suis subunit antigen, and an amino acid sequence encoding the protein. The invention also relates to and providesa streptococcus suis vaccine composition, and the vaccine composition is used for the prevention and / or treatment of diseases associated with streptococcus suis and infections caused by streptococcus suis.

Description

technical field [0001] The invention relates to the field of veterinary biological products. More specifically, the present invention relates to a Streptococcus suis vaccine composition and a method for preparing the same, said composition comprising two Streptococcus suis immune antigens. The present invention also relates to the use of the immune antigen in the preparation of compositions and methods for preventing and / or treating diseases related to Streptococcus suis and infections caused by Streptococcus suis. Background technique [0002] Streptococcus suis is an important pathogen of pigs, causing many pathological diseases such as arthritis, endocarditis, meningitis, pneumonia and septicemia. It is also an important zoonotic pathogen for humans exposed to contaminated pigs or their by-products, causing meningitis and endocarditis. Thirty-three serotypes (types 1-31, 33, and 1 / 2) based on capsular antigens are currently known, but the mechanisms involved in the path...

Claims

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Application Information

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IPC IPC(8): A61K39/09A61P31/04
Inventor 张许科孙进忠张海洋田克恭
Owner PU LIKE BIO ENG
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