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Method for reducing fluorine content in water soluble active peptides from Antarctic krill

An Antarctic krill and active peptide technology, applied in fermentation and other directions, can solve the problems of complex equipment, low solubility, high cost, etc., and achieve the effects of less investment in equipment, high product safety, and high product recovery rate

Inactive Publication Date: 2014-12-24
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The foreign patent UK2240786A discloses a method for reducing the fluorine content of Antarctic krill by enriching with aluminum electrodes, but this method has the problem of aluminum residue; the domestic patent CN101690538B discloses a method for preparing low-fluorine protein base material by pickling method. The method uses the low solubility of protein at the isoelectric point (weak acid condition) to recover the protein, which is not suitable for low molecular weight polypeptides with good water solubility; domestic patent CN102524510B discloses a method for preparing a low-fluorine protein base material. Solution pH or heating to recover protein is not suitable for low-molecular-weight polypeptides with good water solubility; domestic patent CN102532255A discloses a method for preparing low-fluorine Antarctic krill shrimp meat protein using ion-exchange membrane electrolysis and electroosmosis. The recovery of shrimp meat protein by solid-liquid separation is not suitable for low-molecular-weight polypeptides with good water solubility; domestic patent CN102106493B discloses a method for preparing low-fluorine Antarctic krill meal by adjusting acid and reducing and evaporating cycle defluorination. The equipment used in this method is complex and expensive

Method used

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  • Method for reducing fluorine content in water soluble active peptides from Antarctic krill
  • Method for reducing fluorine content in water soluble active peptides from Antarctic krill

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1) Take 5kg of Antarctic krill powder, add 60L of distilled water to the krill powder, add 2M HCl to the mixed solution, adjust the pH value of the mixed solution to 7.5, heat up to 45°C, add 75g of neutral protease to the mixed solution, and Stir in the reaction kettle at constant temperature for 10 hours, heat to 100°C to terminate the enzyme reaction, and centrifuge to obtain supernatant A;

[0023] 2) Make the solid content of the supernatant A reach 15% by vacuum concentration, the vacuum degree is 0.08MPa, and the temperature is 40-80°C to obtain solution B;

[0024] 3) Take 10L of solution B, add 100g of calcium hydroxide to it, mix well, and cool to obtain mixed solution C;

[0025] 4) Add 184 g of monobasic calcium phosphate (calcium hydrogen phosphate) to the mixed solution C, and stir while adding to obtain the mixed solution D;

[0026] 5) After the mixture D is centrifuged at 5000r / min, the supernatant E is obtained;

[0027] 6) The supernatant E was spra...

Embodiment 2

[0033] 1) Take 10 kg of Antarctic krill powder, add 100 L of 95% ethanol to the krill powder, stir in the reaction kettle for 6 hours, centrifuge to take the precipitate, and dry it in vacuum to obtain defatted krill powder;

[0034] 2) Take 5kg of defatted shrimp powder, add 50L of distilled water to the defatted shrimp powder, add 2M HCl to the mixture, adjust the pH value of the mixture to 8.0, raise the temperature to 50°C, add 80g of alkaline protease to the mixture, and hydrolyze at constant temperature 8h, heat to 100°C to stop the enzyme reaction, centrifuge to get the supernatant A;

[0035] 3) Make the solid content of the supernatant A reach 10% by vacuum concentration, the vacuum degree is 0.08MPa, and the temperature is 40-80°C to obtain solution B;

[0036] 4) Take 10L of solution B, add 80g of calcium oxide to it, mix well, and cool to obtain mixed solution C;

[0037] 5) Add 334 g of calcium dihydrogen phosphate to the mixed solution C, and stir while adding, ...

Embodiment 3

[0042] 1) Take 10 kg of Antarctic krill powder, add 100 L of 95% ethanol to the krill powder, stir in the reaction kettle for 6 hours, centrifuge to take the precipitate, and dry it in vacuum to obtain defatted krill powder;

[0043] 2) Take 5kg of defatted shrimp powder, add 50L of distilled water to the defatted shrimp powder, raise the temperature to 50°C, add 2M HCl to the mixture to adjust the pH value of the mixture to 8.0, add 80g of alkaline protease to the mixture, and hydrolyze for 8 hours at constant temperature , heated to 100°C to terminate the enzyme reaction, and centrifuged to get the supernatant A;

[0044] 3) Make the solid content of the supernatant A reach 10% by vacuum concentration, the vacuum degree is 0.08MPa, and the temperature is 40-80°C to obtain solution B;

[0045] 4) Take 10L of solution B, add 150g of calcium chloride to it, mix well, and cool to obtain mixed solution C;

[0046] 5) After the mixture C is centrifuged at 4500r / min, the supernata...

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Abstract

The invention provides a method for reducing the fluorine content in water soluble active peptides from Antarctic krill. The method comprises the steps of carrying out protease hydrolysis on Antarctic krill powder to obtain a krill active peptide aqueous solution; mixing uniformly after a defluorination agent I and a defluorination agent II are sequentially added to the krill active peptide aqueous solution; centrifugally separating the active peptide aqueous solution to obtain a defluorinated supernatant; and carrying out spray drying on the defluorinated supernatant to obtain the product of water soluble Antarctic krill active peptide powder with low fluorine content. The method provided by the invention is simply operated, needs less equipment investment, has no environment pollution and high product recovery rate, and is suitable for industrial operation; the krill active peptide powder with low fluorine content obtained according to the method has the following characteristics that the fluorine content is reduced from 350-450 ppm to 2-5 ppm, the protein content is greater than 85 percent, no toxic substances are left, and the product safety is high; the content of necessary amino acids is high and meets the amino acid composition pattern modes stipulated in FAO / WHO.

Description

technical field [0001] The invention belongs to the technical field of aquatic product purification, and in particular relates to a method for reducing the fluorine content in water-soluble Antarctic krill active peptide. Background technique [0002] Fluorine has a high degree of biological activity and cannot be automatically degraded in animals and plants. Therefore, excessive intake of fluorine will lead to fluorosis, which will have very adverse effects on health. Excessive fluoride intake can lead to symptoms such as dental fluorosis, skeletal fluorosis, joint pain, ligament calcification, bone hyperplasia, and inconvenient movement. In severe cases, it can also cause curvature of the spine and even paralysis. In view of the potential harm of fluorine to the human body, the Ministry of Health of my country stipulates that the allowable daily intake of fluorine for adults is 3.5mg. [0003] Antarctic krill is a class of marine invertebrates distributed in Antarctic wat...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 薛长湖王彦超薛勇常耀光王静凤徐杰唐庆娟
Owner OCEAN UNIV OF CHINA
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