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Construction method and application of immobilized biological bacterium agent for micro-polluted water source

A technology of biological bacterial agent and construction method, which is applied in the construction and application field of immobilized biological bacterial agent in micro-polluted water sources, and can solve the problems of poor treatment effect of algae and algal toxins

Inactive Publication Date: 2014-12-24
松辽流域水资源保护局松辽流域水环境监测中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a construction method and application of immobilized biological agents in micro-polluted water sources, so as to solve the problem of poor treatment effects of ammonia nitrogen, organic matter, iron, manganese, algae and algal toxins in water source water by existing biological methods

Method used

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specific Embodiment approach 1

[0031] Specific embodiment one: The construction method of a kind of slightly polluted water source immobilized biological bacterial agent of this embodiment is carried out according to the following steps:

[0032] (1) Re-screening and compounding of multifunctional oligotrophic bacteria: After fermenting high-efficiency COD-degrading bacteria, ammonia-nitrogen bacteria and phosphorus-removing bacteria separately, the concentration of the above three bacteria was maintained at a CFU of 10 6each / mL, and then mixed according to the volume ratio of 2:2:1 to obtain multifunctional oligotrophic bacteria; wherein, the high-efficiency COD degrading bacteria, ammonia nitrogen bacteria and phosphorus removal bacteria are all activated carbon domesticated from oligotrophic After being obtained on the biofilm, it is separated and obtained through starvation resistance experiments and purification effect experiments;

[0033] (2) Screening of high-efficiency algae-dissolving bacteria: un...

specific Embodiment approach 2

[0038] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that when high-efficiency COD degrading bacteria are separated by starvation resistance test and purification effect test, the required culture medium is composed of 0.5g / L glucose, 0.5g / L NHCl, 0.5g / L KH 2 PO 4 , 0.5g / L of K 2 HPO 4 , 0.05g / L of CaCl 2 , 0.5g / L of MgSO 4 ·7H 2 O, the trace element solution of 1mL / L, the yeast extract of 2g / L and the water of balance are made up of, and wherein, described trace element solution is the CoCl of 0.2g / L 2 ·6H 2 O, 0.3g / L MnCl 2 4H 2 O, 0.04g / L ZnCl 2 , 0.01g / L NiCl 2 ·6H 2 O, 0.02g / L CuSO 4 ·5H 2 O, 0.01g / L Na 2 MoO 4 2H 2 O, 0.01g / L Na 2 SeO 4 2H 2 O and 0.2g / L FeSO 4 Composition, the pH of the medium is 7.0-7.5. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0039] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: when ammonia-nitrogen bacteria are separated by starvation resistance test and purification effect test, the required culture medium is composed of 0.5g / L glucose, 1.0g / L NHCl, 1.0g / L NaNO 3 , 0.5g / L of KH 2 PO 4 , 0.5g / L of K 2 HPO 4 , 0.05g / L of CaCl 2 , 0.5g / L of MgSO 4 ·7H 2 O, the trace element solution of 1mL / L, the yeast extract of 2g / L and the water of balance are made up of, and described trace element solution is made of the CoCl of 0.2g / L 2 ·6H 2 O, 0.3g / L MnCl 2 4H 2 O, 0.04g / L ZnCl 2 , 0.01g / L NiCl 2 ·6H 2 O, 0.02g / L CuSO 4 ·5H 2 O, 0.01g / L Na 2 MoO 4 2H 2 O, 0.01g / L Na 2 SeO 4 2H 2 O and 0.2g / L FeSO 4 Composition, the pH of the medium is 7.0-7.5. Others are the same as in the first or second embodiment.

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Abstract

The invention discloses a construction method and an application of an immobilized biological bacterium agent for a micro-polluted water source and belongs to the field of ecological remediation of water environments. The method is a treating process for purifying a water body through construction of the biological bacterium agent, and ammonia nitrogen, iron, manganese, microcystis, algal toxins and the like in the water body can be removed effectively. The method comprises steps as follows: (1) rescreening and compounding of multifunctional oligotrophic ammonia nitrogen bacteria; (2) screening of efficient algicidal bacteria; (3) fermentation and construction of the bacterium agent; (4) bacterium agent immobilization and biological enhancement; (5) research on dosing methods of the bacterium agent, and treatment and biological enhancement of the micro-polluted drinking water source. The biological bacterium agent is constructed with an immobilization technology, after a system runs stably, the removal rate of the ammonia nitrogen is higher than 80%, removal rates of the microcystis and the algal toxins are higher than 40%, the removal rate of the iron in water is 50%, and the removal rate of manganese is 51%. The method can be combined with actual projects and is wider in application prospect.

Description

technical field [0001] The invention relates to the construction and application of a slightly polluted water source immobilized biological bacterial agent, which belongs to the development of environmental sewage treatment bacterial agent and its practical engineering application method. technical background [0002] Water quality pollution of drinking water sources at this stage mainly includes organic matter (natural organic matter (NOM) and synthetic organic matter (SOC)), nitrogen (often present in water in the form of organic nitrogen, ammonia, nitrite and nitrate), phosphorus, smell, taste, Three substances, iron, manganese, etc. Generally speaking, industrially polluted water bodies mainly include petroleum hydrocarbons, volatile phenols, ammonia nitrogen, pesticides, COD, heavy metals, arsenic, cyanide, etc. These pollutants have many types, complex properties, and great harm, especially those that are difficult to degrade, Toxic organic pollutants that are easy to...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/07C12R1/40C12R1/38C12R1/13C12R1/01
Inventor 郑国臣张静波魏民李环魏利项丽君
Owner 松辽流域水资源保护局松辽流域水环境监测中心
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