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Bacillus subtilis and application thereof

A technology of Bacillus subtilis and strawberry root rot, applied in the direction of application, bacteria, fungicides, etc., can solve problems such as rot, affecting product quality and health, and strawberry death

Inactive Publication Date: 2014-12-24
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the harm of strawberry root rot (Fusarium oxsporum) is the most serious, causing root rot, which can directly lead to the death of strawberries and cause great economic losses.
The prevention and control of this disease mostly uses chemical agents to spray the plant surface, resulting in varying degrees of pesticide residues and pollution in the soil, affecting product quality and people's health

Method used

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  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0043] Bacillus subtilis (B85) against strawberry root rot (Fusarium oxsporum), strawberry gray mold (Botrytis fabae), strawberry anthracnose (Colletotrichum gloeosporiodes), Dendrobium officinale gray mold (Botryotinia fuckeliana) and wheat scab ( Plate antibacterial effect of Fusarium asiaticum).

[0044] The Bacillus subtilis (Bacillus subtilis) B85 bacterial strain activated on the LB slope was streak-inoculated on one side of a 90mm PDA plate, and then the strawberry root rot, strawberry gray mold, strawberry anthracnose, Dendrobium botrytis cinerea and wheat gibberella Use a puncher with a diameter of 5mm to get the same quality and amount of bacteria blocks from the PDA plate culture of pathogenic bacteria, and inoculate them on the other side of the plate. Cultivate at 30±1°C for 14 days and 28 days, observe the antibacterial ability, and record the width of the inhibition zone (as shown in Table 1), the experimental effect is as follows: Figure 2a to Figure 2e shown...

experiment example 2

[0049] Experimental example 2: Bacillus subtilis (Bacillus subtilis) B85 metabolites to strawberry root rot (Fusarium oxsporum), strawberry gray mold (Botrytis cinerea), strawberry anthracnose (Colletotrichum gloeosporiodes), wheat scab (Fusarium asiaticum) Plate antibacterial effect.

[0050] The fermented liquid of Bacillus subtilis (Bacillus subtilis) B85 cultivated for 3 days was filtered with a bacterial filter, and the filter membrane aperture of the bacterial filter was 0.25 μm; the PDA plate (diameter 90 mm) culture of 5 kinds of plant pathogenic fungi was punched with a diameter of 5 mm. Punch out the same quality and the same amount of bacterial blocks with the puncher, and inoculate them on one side of the PDA plate; then use a 5mm diameter puncher on the other side of the PDA plate to punch out the same quality and the same amount of round blocks, and take out the round blocks; Connect 100 μL of sterile filtrate of Bacillus subtilis B85 after filtration to the cavi...

experiment example 3

[0059] Experimental Example 3: A test to verify whether Bacillus subtilis (Bacillus subtilis) B85 has an inhibitory effect on strawberry root rot.

[0060] 1. Experimental equipment

[0061] Ultra-clean workbench, PRX-250A intelligent artificial climate box, Peiying brand DHZ-DA TCYQ constant temperature oscillator.

[0062] 2. Experimental principle

[0063] Before strawberry cultivation, wash the roots of strawberry seedlings with clean water, dry them, soak them in the dilution of Bacillus subtilis (B.subtilis) B85 for 2-3 minutes, take them out and dry them, Bacillus subtilis B85 can stick Attached to strawberry roots to continue to grow and reproduce, produce metabolites, thereby inhibiting the growth of strawberry root rot bacteria.

[0064] 3. Experimental method:

[0065] 1) Cultivation of Bacillus subtilis (B.subtilis) B85 bacterial liquid: Inoculate Bacillus subtilis (B.subtilis) B85 into a Erlenmeyer flask containing 500 mL of LB medium, and culture at 35°C for 6...

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Abstract

The invention discloses bacillus subtilis. The bacillus subtilis with the preservation number of CGMCC No.7542 is preserved in the China General Microbiological Culture Collection Center on April 28, 2013. The invention further provides an application of the bacillus subtilis in strawberry root rot. The bacillus subtilis B85 has a very good inhibition effect on strawberry root rot, grey mould fruit rot of strawberry, strawberry anthracnose and other pathogenic fungi, and also has a very good preventing and controlling effect on other plant diseases. The bacillus subtilis has a high proliferation speed, can be artificially cultured, is easy to operate, facilitates production and application, has the advantages of strong stress resistance and the like, especially has an important meaning to prevention and control of fungi caused strawberry root rot, is simple in culture condition and easy to preserve, is convenient for industrial production, and has excellent development and application prospects.

Description

technical field [0001] The invention belongs to the field of plant protection, in particular to a Bacillus subtilis B85 and application thereof. Background technique [0002] In recent years, with the general concern of the public on the safety of chemical pesticides in food and the environment, and the prohibition of some dangerous chemical pesticides, people are seeking new ways to control plant diseases safely and effectively, and the use of beneficial microorganisms to control plant diseases has attracted increasing attention. It introduces exogenous antagonistic bacteria beneficial to plants into the broken plant natural ecosystem, or promotes the growth of natural beneficial microbial populations by regulating the environment, and exhibits biological control activity, and achieves disease prevention through the interaction between microorganisms Purpose. At present, the biocontrol of plant diseases has become the research focus of various countries. [0003] There ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/125
Inventor 赵桂华汪善锋徐超杨鹤同
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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