Preparation method of controllable hydrophobic bacterial cellulose-zein composite film
A technology of zein and bacterial cellulose membrane, which is applied in medical science, absorbent pads, surgery, etc., can solve the problems of high cost, no controllability, and limited surface modification effect of bacterial cellulose membrane, and achieve cost Low, enhanced cell adhesion properties, excellent mechanical properties and biocompatibility effects
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Embodiment 1
[0035] Step 1, preparation of bacterial cellulose membrane
[0036] Medium composition: 2.0% D-glucose, 0.5% peptone, 0.5% yeast extract, 0.115% citric acid, 0.27% Na 2 HPO 4 12H 2 O, the rest is distilled water, pH5.0.
[0037] Cultivation method: inoculate Acetobacter xylinum into the seed culture solution, and vibrate at 30°C for 12 hours, then inoculate 10% of the fermentation medium, shake well, and culture at 30°C for 5 days to obtain a bacterial cellulose film.
[0038] Step 2, purification of bacterial cellulose membrane
[0039] Take the bacterial cellulose membrane prepared above, wash it with water for 3 times, remove the culture medium, impurities and bacteria on the surface of the membrane; then soak the bacterial fiber wet membrane in a NaOH solution with a concentration of 0.2mol / L, at 90°C Take it out after boiling for 30 minutes, remove the culture medium and cells remaining in the film, then soak the film in distilled water, boil at 90°C for 30 minutes, c...
Embodiment 2
[0061] Step 1, preparation of bacterial cellulose membrane
[0062] Medium composition: 1.8% D-glucose, 0.45% peptone, 0.45% yeast extract, 0.120% citric acid, 0.25% Na 2 HPO 4 12H 2 O, the rest is distilled water, pH4.8.
[0063] Cultivation method: inoculate Acetobacter xylinum into the seed culture solution, shake and cultivate at 30°C for 12 hours, then inoculate the fermentation medium at a ratio of 15%, shake well, and culture at 30°C for 5 days to obtain bacterial cellulose membrane.
[0064] Step 2, purification of bacterial cellulose membrane
[0065] Take the bacterial cellulose membrane prepared above, wash it with water for 4 times, soak it in NaOH solution with a concentration of 0.4mol / L, boil it at 90°C for 30min, take it out, then soak the membrane in distilled water, and put it under 90°C Boil for 30 minutes, change the water, repeat 4 times, then soak it in distilled water until neutral to obtain the purified bacterial cellulose membrane;
[0066] Step 3...
Embodiment 3
[0091] Step 1, preparation of bacterial cellulose membrane
[0092] Medium composition: 2.2% D-glucose, 0.6% peptone, 0.6% yeast extract, 0.110% citric acid, 0.30% Na 2 HPO 4 12H 2 O, the rest is distilled water, pH 4.0.
[0093] Cultivation method: inoculate Acetobacter xylinum into the seed culture solution, shake and cultivate at 30°C for 12 hours, then inoculate the fermentation medium at a ratio of 12%, shake well, and culture at 30°C for 5 days to obtain bacterial cellulose membrane.
[0094]Step 2, purification of bacterial cellulose membrane
[0095] Take the bacterial cellulose membrane prepared above, wash it with water for 5 times, soak it in 0.2mol / L KOH solution, boil it at 90°C for 30min, take it out, then soak the membrane in distilled water, boil it at 90°C for 30min, replace water, repeated 5 times, and then soaked in distilled water until neutral to obtain the purified bacterial cellulose membrane;
[0096] Step 3, pretreatment of bacterial cellulose mem...
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