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Preparation method of oil soybean meal protein and application of oil soybean meal protein in beef sausage

A technology of oilseed bean meal and oily bean, which is applied in the protein composition, application and food preparation of vegetable seeds, and can solve the research reports on the application of oily soybean meal protein that have not yet been reported, and no one has reported on the nutritional evaluation of oily bean protein and other problems to achieve the effect of improving chewiness

Inactive Publication Date: 2014-12-10
克拉玛依汇利有限责任公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no report on the nutritional evaluation of oilseed bean protein
At present, there is no research report on the application of oily soybean meal protein in beef sausage

Method used

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  • Preparation method of oil soybean meal protein and application of oil soybean meal protein in beef sausage
  • Preparation method of oil soybean meal protein and application of oil soybean meal protein in beef sausage
  • Preparation method of oil soybean meal protein and application of oil soybean meal protein in beef sausage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Preparation of oily sage soybean meal protein

[0034]% is calculated according to the weight percentage, and the ratio of material to liquid is calculated according to the weight ratio. The oily bean cake after supercritical oil extraction is crushed through a 40-mesh to 100-mesh sieve to prepare the oily bean meal, which is degreased and crushed, and then ultrasonically assisted Liquid leaching process, the first alkaline extraction process adopts a material-to-liquid ratio of 1: (15-25) by mass ratio, ultrasonic time is 25 minutes to 45 minutes, pH value is 9-11, and the isoelectric point of oily soybean meal protein is pI= 3.8-4.4; the ratio of the second to the fifth alkali extraction to the material liquid is 1:10 by mass ratio, the pH value is 9.6, the ultrasonic time is 26 minutes, and the 3000r / min centrifugation is 15 minutes. Under the conditions of 4.4, acid precipitation for 1 hour, centrifugation at 3,000 r / min for 15 minutes, protein milk was w...

Embodiment 2

[0036] Example 2: Preparation of oily sage soybean meal protein

[0037] % is calculated according to the weight percentage, and the ratio of material to liquid is calculated according to the weight ratio. The oily soybean cake after supercritical oil extraction is crushed through a 40-mesh sieve to prepare the oily soybean meal. Extraction process, the first alkaline extraction process adopts a material-to-liquid ratio of 1:17 by mass ratio, an ultrasonic time of 26 minutes, a pH value of 9.6, an extraction rate of 44.01%, and the isoelectric point of oily soybean meal protein is pI=4.2; the second, The ratio of the three times of alkaline extraction to the material solution is 1:10 by mass ratio, the pH value is 9.6, the ultrasonic time is 26 minutes, and the centrifugation is performed at 3000r / min for 15 minutes. The protein milk was washed with 5 times the weight of water at 40°C for 30 minutes, washed 3 times, and freeze-dried to obtain the oily soybean meal protein: the...

Embodiment 3

[0038] Embodiment three: Coomassie brilliant blue standard curve drawing

[0039] 1.1 Coomassie Brilliant Blue standard curve drawing: Prepare 100μg / mL bovine serum albumin, take 0mL, 0.1mL, 0.2mL, 0.3mL, 0.4mL, 0.5mL, 0.6mL respectively in test tubes, fill up to 1mL with 0.15M NaCl solution , and then add 5mL of Coomassie Brilliant Blue solution prepared in advance, after standing for 10min, measure the absorbance at 595nm, and draw the standard curve.

[0040] 1.2 Extraction rate and yield of oily soybean meal protein

[0041] Extraction rate of oily soybean meal protein = protein content in extract ÷ crude protein content in raw material × 100%

[0042] Protein yield of oily soybean meal = (protein sample weight × crude protein content) ÷ (raw material weight × crude protein content)

[0043] The crude protein content in oily soybean meal and oily soybean protein powder was determined by the Kjeldahl method N×6.25, and the protein content in the extract was determined by ...

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Abstract

The invention discloses a preparation method of oil soybean meal protein and an application of oil soybean meal protein in a beef sausage. The preparation method of the oil soybean meal protein comprises the following steps: carrying out supercritical oil extraction on cyperus esculentus, caking, crushing, screening, degreasing and adopting three times of ultrasonic auxiliary alkali leaching processes, wherein the first alkaline leaching process adopts the ratio of materials to liquid of 1 to 17, the ultrasound time of 26 minutes and the pH value of 9.6, and the second and the third alkaline leaching processes adopt the ratio of the materials to the liquid of 1 to 10, the pH value of 9.6, and the ultrasound time of 26 minutes; rotating by 3000 r / minute, centrifuging for 15 minutes, combining an alkali leaching liquid obtained in three times, carrying out acid precipitation for 1 hour under the condition that the pH value is 4.2, rotating by 3000 r / minute, centrifuging for 15 minutes, washing protein milk for 30 minutes by using 40-DEG C water with a weight of 5 times of the protein milk, washing for three times, and freezing and drying to obtain the oil soybean meal protein. The preparation method of the oil soybean meal protein and the application of the oil soybean meal protein in the beef sausage have the benefits that the yield of the applied beef sausage is improved by 21.59 percent, which is improved by 11.52 percent compared with the applied beef sausage added with soybean protein; the chewiness, the hardness, elasticity and other sausage gel indicators of the beef sausage can be obviously improved, and the application is wide.

Description

technical field [0001] The invention relates to the technical field of preparation of soybean meal protein. Specifically, the present invention relates to the technical field of preparation and application of oily soybean meal protein. Background technique [0002] Oil Shadou ( Cyperus esculentus L. ), also known as oil sedge, iron water chestnut, is a C4 plant of Cyperaceae Cyperaceae. Oil Shadou English common name is Cyperus esculentus, Chufa Flatsedge, Tigernut Sedge, Yellow Nutsedge, Yellow Nutgrass, Yellow Umbrella-sedge and Earthalmond wait. Salsa bean is a perennial weed that has historically been common in tropical and Mediterranean regions. Almonds produce nut-like underground tubers, a tuberous vegetable known as "subterranean almonds". It is a healthy food, and it has been reported that it can prevent heart disease, thrombosis, reduce the risk of colon cancer and activate blood circulation. Yasha bean tubers are rich in starch, fat, sucrose and protein e...

Claims

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Application Information

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IPC IPC(8): A23J1/14A23L1/305
Inventor 敬思群陈政辉王耀祥
Owner 克拉玛依汇利有限责任公司
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