Simultaneous extraction, enrichment and quantification of six trace quinolone antibiotics on suspended particulate matter in water

Abstract

The invention discloses a method for simultaneously extracting, enriching and quantifying six trace quinolone antibiotics on the suspended particulate matters in water. The method comprises the steps of filtering a target water sample by a microporous fiber membrane; after filtering, collecting a filter membrane, drying the filter membrane in the air, cutting the dried filter membrane into pieces, and putting the pieces into a triangular flask; feeding an extraction agent into the triangular flask, sealing, oscillating and carrying out ultrasonic extraction so as to obtain extract liquid; filtering the extract liquid by an organic filter membrane, and transferring the filtered extract liquid into a K-D concentration flask; feeding a dewatering desiccant into the extracted liquid, sucking the water dry, putting the K-D concentration flask into a rotary evaporator, and carrying out concentration so as to obtain concentrated liquid; purging the concentrated liquid by nitrogen until the volume of the liquid is less than 1ml; making up to the volume of the concentrated and extracted liquid to 1ml, and then transferring the liquid into an Agilent special bottle; detecting by adopting an internal standard method, and a high performance liquid chromatography (HPLC)-tandem three-stage mass spectrum combination instrument; analyzing a chromatography mass spectrometry analysis diagram to complete the detection. After the method is used, the content of the trace quinolone absorbed on suspended particulate matters in a water environment is supplemented, so that the blank of antibiotics detection is made up.

Description

technical field [0001] The invention relates to a detection method of quinolone antibiotics in water environment, specifically relates to a quantitative method for extraction and enrichment of multi-component quinolone antibiotics on particulate matter in water, and belongs to the technical field of environmental monitoring. Background technique [0002] Quinolones, also known as pyroxic acids or pyridonic acids, are synthetic antibacterial drugs containing the basic structure of 4-quinolone. Because of its broad antibacterial spectrum and strong antibacterial activity, this type of drug is widely used in human clinical medicine, animal husbandry, aquaculture and other aquaculture industries. Quinolones are divided into four generations, and the most clinically used one is the third generation. Commonly used drugs include norfloxacin, ofloxacin, and ciprofloxacin. These drugs have a bactericidal effect on a variety of Gram-negative bacteria and have weaker effects on Gram-p...

AI Technical Summary

Problems solved by technology

These methods are limited to the simultaneous quantification of single substances or multiple components of dissolved quinolones

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