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Culture medium supplementary method for producing colomycin by fermentation of panebacillus polymyxa

A fermentation medium and colistin technology, applied in the field of fermentation, can solve the problems of glucose carbonization, reducing reducing sugar content, long fermentation culture cycle, etc., and achieve the effect of reducing production cycle and increasing fermentation unit

Active Publication Date: 2014-11-19
宁夏泰瑞制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005]2 Glucose is added to the culture medium for domestic fermentation and production of colistin, and after high-temperature sterilization, it is easy to cause part of the glucose to be carbonized, reduce the content of reducing sugar, and affect the potency of the fermentation broth
[0006]3 Domestic colistin fermentation and cultivation period is relatively long, usually over 120 hours, resulting in high energy consumption
[0007]4 Fermentation costs are high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Preparation of seed medium: the volume of seed liquid is 1m 3

[0056] Seed medium: 5kg of bran, 10kg of malt extract, 2kg of fish meal, 11L of corn steep liquor, 15kg of baked soybean powder, 10kg of brewer’s yeast extract, 1kg of ammonium sulfate, 1kg of light calcium carbonate, and 3L of olive oil.

[0057] First sterilize the seed medium and cool it down to 25°C, and keep the pressure with sterile air, and then under the protection of the flame, the cultured Paenibacillus polymyxa is 0.3L / m 3 The inoculum amount was inserted into the seed medium for seed culture, and transplanted when the bacterial cell concentration was 30%, the pH value was 7.8, and the cultivation time was 30 hours, and the transplantation ratio was controlled at 0.8m 3 / m 3 ;

[0058] Preparation of fermentation medium: the volume of fermentation broth is 10m 3

[0059] Fermentation medium: 90kg of bran, 150kg of malt extract, 40kg of fish meal, 150L of corn steep liquor, 200kg of baked s...

Embodiment 2

[0062] Preparation of seed medium: the volume of seed liquid is 1m 3

[0063] Seed medium: 6kg of bran, 11kg of malt extract, 3kg of fish meal, 12L of corn steep liquor, 16kg of baked soybean powder, 11kg of brewer’s yeast extract, 2kg of ammonium sulfate, 2kg of light calcium carbonate, 4L of rapeseed oil.

[0064] First, sterilize the seed medium and cool it down to 26°C, and keep the pressure with sterile air, and then under the protection of the flame, the Paenibacillus polymyxa that has been cultivated is 0.35L / m 3 The amount of inoculum was inserted into the seed medium for seed culture, and transplanted when the cell concentration was 32%, the pH value was 7.4, and the cultivation time was 35 hours, and the transplantation ratio was controlled at 0.85m 3 / m 3 ;

[0065] Preparation of fermentation medium: the volume of fermentation broth is 10m 3

[0066] Fermentation medium: bran 100kg, malt extract 160kg, fish meal 50kg, corn steep liquor 160L, baked soybean po...

Embodiment 3

[0069] Preparation of seed medium: the volume of seed liquid is 1m 3

[0070] Seed medium: 7kg of bran, 12kg of malt extract, 4kg of fish meal, 13L of corn steep liquor, 17kg of baked soybean powder, 12kg of brewer’s yeast extract, 3kg of ammonium sulfate, 3kg of light calcium carbonate, and 5L of olive oil.

[0071] First, sterilize the seed medium and cool it to 27°C, and keep the pressure with sterile air, and then under the protection of the flame, the Paenibacillus polymyxa that has been cultivated is 0.4L / m 3 The inoculum amount was inserted into the seed medium for seed culture, and transplanted when the bacterial cell concentration was 35%, the pH value was 6.9, and the cultivation time was 40 hours, and the transplantation ratio was controlled at 0.9m 3 / m 3 .

[0072] Preparation of fermentation medium: the volume of fermentation broth is 10m 3

[0073] Fermentation medium: bran 110kg, malt extract 170kg, fish meal 60kg, corn steep liquor 170L, baked soybean p...

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Abstract

The invention relates to a culture medium and supplementary method for producing colomycin by fermentation of panebacillus polymyxa. By arranging a formula of a seed medium and a fermentation medium which are suitable for producing colomycin by the fermentation of panebacillus polymyxa, and a fermentation supplementary process, fermentation unit of colomycin is increased; a fermentation period is shortened; fermentation cost is reduced; influences of the source of raw materials and auxiliary materials on fermentation titer is reduced at the maximum degree; the source of the raw materials and auxiliary materials is not influenced by an environment; sufficient supply is guaranteed; and stable and efficient production of colomycin can be realized.

Description

technical field [0001] The invention belongs to the field of fermentation technology, and in particular relates to a culture medium and feeding method for producing colistin by Paenibacillus polymyxa fermentation. Background technique [0002] Colistin is a basic polypeptide system antibiotic obtained from the culture medium of polymyxa, which is a mixture of polymyxin E1 and E2. It is a white powder, easily soluble in water, heat-resistant, difficult to absorb in the digestive tract, excreted quickly, has low toxicity, no side effects, and is not easy to produce drug-resistant strains. It is one of the safest growth-promoting antibiotics for livestock and poultry. Colistin mainly has a strong antibacterial effect on Gram-negative bacteria, is effective against almost all Gram-negative bacteria, and has a significant effect on Pseudomonas aeruginosa. Treatment is often combined with bacitracin zinc against Gram-positive bacteria; used as a feed additive to stimulate the gro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02C12R1/12
Inventor 任勇
Owner 宁夏泰瑞制药股份有限公司
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