Method for analyzing night cold flu cough allergy capsule by utilizing HPLC (High Performance Liquid Chromatography)
A high-performance liquid chromatography, soft capsule technology, applied in the field of drug analysis, to achieve the effects of improving peak shape, increasing response, and increasing retention
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Embodiment 1
[0074] Chromatographic conditions:
[0075] High performance liquid chromatography: Waters e2996, PDA detector; Chromatographic column: Agilent SB-C18, 4.6×150mm, 5μm; Sodium octane sulfonate concentration is 3 mmol / L sodium octane sulfonate-phosphate buffer solution (weigh 0.65g sodium octane sulfonate, 3.12g sodium dihydrogen phosphate into 1L water, adjust pH to 2.5 with phosphoric acid, degas, filter) as mobile phase A, using acetonitrile as mobile phase B, carry out gradient elution, Gradient elution conditions are shown in Table 1; flow rate: 1.2 ml / min; detection wavelength: 275 nm; column temperature: 35°C; injection volume: 20 μL.
[0076] Table 1
[0077] time (min)
Mobile phase A (V%)
Mobile phase B (V%)
0
90
10
1
90
10
16
50
50
17
90
10
21
90
10
[0078] Experimental steps:
[0079](1) Preparation of contrast solution: Accurately weigh 65 mg of paracetamol referenc...
Embodiment 2
[0083] Chromatographic conditions:
[0084] High-performance liquid chromatography: Waters e2996, PDA detector; chromatographic column: Agilent SB-C18, 4.6 × 150mm, 5 μm; sodium octane sulfonate-phosphate buffer with a concentration of sodium octane sulfonate of 3 mmol / L Solution (weigh 0.65g sodium octane sulfonate, 3.12g sodium dihydrogen phosphate into 1L water, adjust pH to 3.0 with phosphoric acid, degas, filter) as mobile phase A, use acetonitrile as mobile phase B, carry out gradient elution , gradient elution conditions are shown in Table 1; flow rate: 1.2 ml / min; detection wavelength: 278nm; column temperature: 35°C; injection volume: 20 μL.
[0085] Experimental procedure: with embodiment 1.
[0086] Its HPLC spectrum is as Figure 4~6 .
Embodiment 3
[0088] Chromatographic conditions:
[0089] High-performance liquid chromatography: Waters e2996, PDA detector; chromatographic column: Agilent SB-C18, 4.6×150mm, 5μm; sodium octanesulfonate-phosphate buffer with a concentration of sodium octanesulfonate of 2 mmol / L Solution (weigh 0.43g sodium octane sulfonate, 3.12g sodium dihydrogen phosphate into 1L water, adjust pH to 2.5 with phosphoric acid, degas, filter) as mobile phase A, use acetonitrile as mobile phase B, carry out gradient elution , gradient elution conditions are shown in Table 1; flow rate: 1.2 ml / min; detection wavelength: 275nm; column temperature: 35°C; injection volume: 20 μL;
[0090] Experimental procedure: with embodiment 1.
[0091] Its HPLC spectrum is as Figure 7-9 .
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