Haemophilus culture medium and a preparation method thereof

Abstract

The invention provides a haemophilus culture medium and a preparation method thereof. The haemophilus culture medium comprises the following components by weight percent: 0.3-3% of Columbia agar, 0.001-0.003% of X factor, 0.001-0.003% of Y factor and 0.0005-0.002% of bacitracin disk antibacterial agent, and the culture medium is prepared by heating and mixing the components; the culture medium is rich in nutrition, the consistency of culture capacity is high, the growth of bacteria is easily observed, by maintaining the normal activity of the factor V and factor X under specific conditions, Haemophilus influenzae grows stably, the culture medium has strong anti-bacterial capability, the haemophilus can be effectively separated, the cost is greatly reduced, the direct cost is equivalent to only about 30% of that of foreign modified chocolate agar; and the preparation method is simple and suitable for the requirement on large-scale industrial production.

Description

technical field [0001] The invention relates to a culture medium and a preparation method thereof, in particular to a haemophilus culture medium and a preparation method thereof. Background technique [0002] Contemporary infection medicine believes that Haemophilus influenzae is the main pathogenic bacteria of upper and lower respiratory tract infections, pneumonia, otitis media, eyes and eye infections. However, the bacteria are fastidious bacteria and cannot be detected by conventional bacterial culture. They need special medium containing X and V factors to be detected. Commonly used is the chocolate medium added with defibrated sheep blood or the modified chocolate medium popular in the world. They are all based on a brown background, so when observing the growth of Haemophilus, it is difficult to observe because the color is dark and opaque. [0003] The medium commonly used in the past for the isolation and cultivation of Haemophilus in China is chocolate agar medium...

AI Technical Summary

Problems solved by technology

[0005] 1. The collection of sterile defibrinated sheep blood is time-consuming and laborious;

[0006] 2. During the destruction of blood cells, the degree of destruction of different sheep blood is not the same, and it is easy to have differences between batches;

[0007] 3. During the preparation process, if the blood is not shaken in time, larger particles will be formed, and a large number of air bubbles will easily appear when shaking, which will cause great trouble for pouring;

[0008] 4. The culture medium itself is darker in color, and it is difficult to observe the formed colonies

However, the dosage of bacitracin is very large, reaching 300mg / L, and the cost is very high