Method for rapidly sensitively detecting fentanyl in blood

A blood and sensitive technology, applied in the direction of measuring devices, preparation of test samples, material analysis by electromagnetic means, etc., can solve the problems of popularization, application limitations, experimental repeatability, poor selectivity, etc., and achieve simple sample pretreatment, The effect of fast detection speed and high sensitivity

Inactive Publication Date: 2014-10-22
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the special reagents required for the detection of plasma fentanyl by immunoassay are time-sensitive, and the repeatability and selectivity of the experiment are poor. Most of the test results need to be verified, which limits the promotion and application of this method.

Method used

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  • Method for rapidly sensitively detecting fentanyl in blood
  • Method for rapidly sensitively detecting fentanyl in blood
  • Method for rapidly sensitively detecting fentanyl in blood

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Experimental program
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Embodiment 1

[0036] figure 2 In the positive ion mode, the air reagent ion peak appears at about 6.3ms; under the detection conditions, a 10μL blank blood sample is drawn at the sampling place of the thin slice with a micro-sampler, and the detection signal of the blood sample is at about 6.7ms, such as image 3 Shown; The ion mobility spectrogram of extractant monochlorobutane is as Figure 4 Shown; Compared with the follow-up results, it can be seen that the endogenous substances in the blank blood do not interfere with the detection of fentanyl.

Embodiment 2

[0038] Such as Figure 5 As shown, the ion mobility spectrum of 10 μL, 50 ppm fentanyl standard detected in positive ion mode, the detection signal of fentanyl is at about 8.7ms.

Embodiment 3

[0040] Dissolve 2 mL, 100 ng / mL, and 1000 ng / mL blood samples of fentanyl to be tested in 2 mL of chlorobutane organic solvent, sonicate for 1-3 min, centrifuge for 1-3 min, and take the supernatant for detection; extract 10 μL of the upper The clear liquid is dropped on the sample injection sheet, and the ion mobility spectrometer is used to carry out in the positive ion high pressure mode, and the detection time and peak signal intensity of the ion reaching the ion mobility spectrometer are measured;

[0041] Such as Figure 6-7 , to detect 0.5mg / L and 0.05mg / L fentanyl samples in 10μL blood, the detection signal of fentanyl is about 8.7ms, but the signal intensity is different at different concentrations.

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Abstract

The invention discloses a method for rapidly sensitively detecting fentanyl in blood. An ion migration spectrum technology is taken as a basic detection technology, a positive-ion high-voltage mode is employed, an ion migration spectrum is utilized as a detection means, a simple solvent extraction pretreatment method is combined, and the method is simple, rapid, efficient and good in reliability. The detection analysis time of a single sample is less than 30 s. The pretreatment time of a sample is less than 5 min, and the sensitivity is high. The measure detection limit can reach 1 ng / mL. The quantitative analysis concentration of the established method comprises the human-body medicine-administration concentration scope (10-100 ng / mL). The detection method is widely applicable to clinical medicine-administration deep analysis, and guides a clinic doctor to precisely administrate a medicine.

Description

technical field [0001] The invention discloses a method for detecting fentanyl in blood. The ion mobility spectrum technology is used as a basic detection technology, and a positive ion mode is adopted to establish a qualitative and quantitative analysis method for rapid detection of fentanyl in blood by ion mobility spectroscopy. The measurement method is simple, fast, efficient and reliable, and can be widely used in medical clinical fentanyl administration concentration analysis to guide doctors in clinical medication. Background technique [0002] Blood is an opaque red liquid that flows in the heart and blood vessels, and its main components are plasma and blood cells. Belongs to connective tissue, the layer of structure in living systems. Blood contains various nutrients, such as inorganic salts, oxygen, and cell metabolites, hormones, enzymes, and antibodies, which have the functions of nourishing tissues, regulating organ activities, and defending against harmful su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64G01N1/28
Inventor 王新李海洋鞠帮玉李杭
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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