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A method for extracting active ingredients of natural products by mixing ethanol and subcritical fluid

A subcritical fluid and natural product technology, which is applied in the extraction of active ingredients of natural products by mixing ethanol and subcritical fluid, and simultaneously extracts water-soluble and fat-soluble active ingredients in natural products. Both water-soluble and fat-soluble active ingredients are extracted, the water-soluble ingredients cannot be fully extracted, and the polysaccharide structure is destroyed, so as to achieve the effect of increasing selectivity and efficiency, short cycle time and high extraction degree

Inactive Publication Date: 2017-05-17
ZHENGZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

201010500500.3 discloses a method for simultaneously extracting multiple bioactive components in yam with subcritical water as a medium. Although the water-soluble yam polysaccharide, allantoin and fat-soluble diosgenin are extracted simultaneously by subcritical water extraction method, continuous extraction is realized, but The extraction process is completed through a three-step heating process, the steps are cumbersome, and the extraction temperature is higher than 100°C, which will destroy the structure of some polysaccharides, thereby affecting the yield
At present, a single subcritical fluid (propane, butane, dimethyl ether, tetrafluoroethane) extraction is especially suitable for the extraction of fat-soluble components in natural products, but cannot achieve sufficient extraction of water-soluble components
[0005] The above different extraction methods have their own advantages and disadvantages. It is difficult to extract both water-soluble and fat-soluble active ingredients. Therefore, it is particularly important to find a low-temperature, efficient, and environmentally friendly extraction method that takes into account both fat-soluble and water-soluble ingredients.

Method used

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  • A method for extracting active ingredients of natural products by mixing ethanol and subcritical fluid

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Embodiment 1

[0030] Take 200g of Robinia pseudoacacia (water content <13%), pulverize and pass through an 80-mesh sieve, place it in a closed extraction tank, add 1800mL of ethanol with a volume fraction of 70%, close the feed channel, evacuate to vacuum, and pour into the extraction tank Dimethyl ether solvent, completely immerse the material, the mass volume ratio (w / v / v) of the added amount of the material, subcritical dimethyl ether and ethanol solution is 1:6:9. At a temperature of 25°C (pressure is the corresponding saturated vapor pressure at the corresponding temperature), and the stirring rate is 700 rpm, the extraction is performed for 50 minutes; after the extraction is completed, slowly put the mixed solvent into the separation tank, and separate the extraction tank and the separation tank. Evaporation and desolvation treatment is carried out to finally obtain an extract containing both fat-soluble components and water-soluble components. The subcritical solvent removed from the...

Embodiment 2

[0032] Take 200g of dried mulberry leaves (water content <13%), pulverize and pass through an 80-mesh sieve, place it in a closed extraction tank, add 400mL of ethanol with a volume fraction of 70%, close the feed channel, pump to vacuum, and extract The dimethyl ether solvent is injected into the tank to completely immerse the materials. The mass-volume ratio (w / v / v) of the added amount of the materials, subcritical dimethyl ether and ethanol solution is 1:13:2. Extract for 30 minutes at a temperature of 45°C (the pressure is the saturated vapor pressure corresponding to this temperature) and a stirring rate of 600 rpm; after the extraction, put the mixed solvent into the separation tank, and evaporate the extraction tank and the separation tank respectively. For processing, inject 400 mL of 70% ethanol into the extraction tank again, evacuate, and inject dimethyl ether solvent for extraction. Repeat three times to obtain an extract containing both fat-soluble components and w...

Embodiment 3

[0035] Take 1000kg of dried eucommia bark (water content <13%), smash it through a 100-mesh sieve, and place it in a closed extraction tank, add 70% ethanol solution, vacuum, and pour butane solvent into the tank Completely immersed, the mass-volume ratio (w / v / v) of the added amount of materials, subcritical butane and ethanol solution is 1:5:5, and the extraction conditions are: time 30min, temperature 30℃ (pressure corresponds to this temperature Saturated vapor pressure), and the stirring rate is 500 rpm. After the extraction, the mixed solvent is put into the separation tank, and the extraction tank and the separation tank are respectively subjected to evaporative desolventizing treatment. After desolventizing is completed, an extract of Eucommia bark containing both fat-soluble and water-soluble components is obtained. The remaining steps are the same as above. Among them, the 200nm inorganic ceramic membrane (alumina membrane) effectively intercepts fat-soluble components...

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Abstract

The invention discloses a method for extracting effective components of natural products and relates to a method for extracting water-soluble and fat-soluble effective components in the natural products simultaneously, particularly to a method for extracting the effective components of the natural products through mixing of ethyl alcohol and a sub-critical fluid, and belongs to the field of extraction of the effective components of the natural products. According to the method which is a new extraction and separation technology, the ethyl alcohol and the sub-critical fluid are mixed to serve as an extracting solvent, in a closed, oxygen-free and low-pressure extracting tank, all the components are separated and purified respectively through three steps, namely, extraction, desolventizing as well as membrane separation-macroporous adsorbent resin combined separation, and finally, high-purity water-soluble component and fat-soluble component extracts of the natural products are obtained; the method has a broad application prospect in the industry.

Description

Technical field [0001] The invention discloses a method for extracting effective ingredients from natural products, in particular to a method for simultaneously extracting water-soluble and fat-soluble effective ingredients from natural products, and in particular to a method for extracting effective ingredients from natural products with a mixture of ethanol and subcritical fluid , It belongs to the field of extraction of active ingredients from natural products. Background technique [0002] The components or their metabolites in animals, plants, insects, marine organisms and microorganisms and many endogenous chemical components in humans and animals are collectively referred to as natural products, which mainly include proteins, polypeptides, amino acids, nucleic acids, and various enzymes , Monosaccharides, oligosaccharides, polysaccharides, glycoproteins, resins, colloids, lignin, vitamins, fats, oils, waxes, alkaloids, volatile oils, flavonoids, glycosides, terpenes, pheny...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D11/02B01D36/00B01D36/04B01D61/58
Inventor 秦广雍王丽庞会利
Owner ZHENGZHOU UNIV
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