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Process for extracting squalene by complex

A squalene and complexing technology, applied in the field of separation and purification of biologically active substances, can solve problems such as no research reports, and achieve the effects of easy dissociation and separation, realization of regeneration, and high efficiency and selectivity.

Inactive Publication Date: 2014-10-08
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has strong selectivity, and is currently mainly used for the separation and purification of unsaturated fatty acids such as high-purity DHA ethyl ester, α-linolenic acid, arachidonic acid, etc., but there is no silver ion complex extraction method for squalene Separate research reports

Method used

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  • Process for extracting squalene by complex

Examples

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Effect test

Embodiment 1

[0028] A kind of technique for extracting squalene by complexation, the method comprises the steps:

[0029] (1) Preparation: wash 1 kg of fresh camellia seed kernels with clean water to remove the dirt attached to the surface, peel and break the shells; mix the resultant with petroleum ether in a mass ratio of 1:5 and ultrasonically (160W, 30min, 3 times) , combined extracts, filtered, concentrated under reduced pressure to obtain Camellia oil-soluble substance concentrated solution (wherein squalene content 10g / L);

[0030] (2) Extraction: Mix the concentrated solution of camellia oil-soluble substances obtained in step (1) with methanol solution of sodium hydroxide, heat in a water bath at 50°C, stir and reflux for 2 hours, wherein the concentration of sodium hydroxide is 0.7mol / L, and the concentration of camellia oil-soluble The volume ratio of the substance concentrate to the methanol solution of sodium hydroxide is 1:20, the fatty acid is removed through saponification ...

Embodiment 2

[0035] A kind of technique for extracting squalene by complexation, the method comprises the steps:

[0036] (1) Preparation: Wash 1 kg of fresh camellia seed kernels with clean water to remove the dirt attached to the surface, peel and break the shells; mix the resultant with petroleum ether in a mass ratio of 1:5 and ultrasonically (200W, 50min, 3 times) , combined extracts, filtered, concentrated under reduced pressure to obtain Camellia oil-soluble substance concentrated solution (wherein squalene content 9g / L);

[0037](2) Extraction: Mix the concentrated solution of camellia oil-soluble substances obtained in step (1) with methanol solution of sodium hydroxide, heat in a water bath at 70°C, stir and reflux for 4 hours, wherein the concentration of sodium hydroxide is 0.9mol / L, and the concentration of camellia oil-soluble The volume ratio of the substance concentrate to the methanol solution of sodium hydroxide is 1:50, the fatty acid is removed through saponification an...

Embodiment 3

[0042] A kind of technique for extracting squalene by complexation, the method comprises the steps:

[0043] (1) Preparation: Wash 1kg of fresh camellia seed kernels with clean water to remove the dirt attached to the surface, peel and break the shells; mix the resultant with n-hexane in a mass ratio of 1:5 and ultrasonically (60W, 20min, 3 times) , combined extracts, filtered, concentrated under reduced pressure to obtain Camellia oil-soluble substance concentrated solution (wherein squalene content 8g / L);

[0044] (2) Extraction: Mix the concentrated solution of camellia oil-soluble substances obtained in step (1) with methanol solution of sodium hydroxide, heat in a water bath at 30°C, stir and reflux for 1h, wherein the concentration of sodium hydroxide is 0.4mol / L, and the concentration of camellia oil-soluble The volume ratio of the substance concentrate to the methanol solution of sodium hydroxide is 1:10, the fatty acid is removed by saponification and methyl esterific...

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Abstract

The invention discloses a process for extracting squalene by complex. The process comprises the following steps of crushing seeds of tea-oil tree, adding petroleum ether, carrying out ultrasonic extraction, filtering, concentrating the filtrate under reduced pressure to obtain a camellia oil fat-soluble substance concentrate; mixing camellia oil fat-soluble substance concentrate with an alcoholic solution of a strong base, heating in a water bath, stirring, refluxing and extracting squalene with petroleum ether to obtain squalene crude product; adding a methanol aqueous solution of silver nitrate into the squalene crude product, under the conditions of oscillation or stirring, carrying out complexation reaction at a low temperature, standing at a low temperature and carrying out liquid separation to obtain an aqueous solution of silver ions-squalene complex as an intermediate product; adding petroleum ether into the aqueous solution of silver ions-squalene complex, heating under reflux, carrying out reverse extraction and then standing and carrying out liquid separation to obtain a dissociated squalene petroleum ether organic phase; cleaning the dissociated squalene petroleum ether organic phase with an aqueous NaCl solution, and then distilling under reduced pressure to recover the organic solvent; cleaning with distilled water again and carrying out vacuum drying to obtain the final product refined squalene.

Description

technical field [0001] The invention belongs to the field of separation and purification of biologically active substances, and in particular relates to a method for complexing and extracting plant squalene using silver ion complexing technology. Background technique [0002] Squalene is a highly unsaturated hydrocarbon compound, a lipid unsaponifiable substance, its chemical name is 2,6,10,15,19,23-hexamethyl-2,6,10,14,18 ,22-Tetracosahexaene is an open-chain triterpenoid compound, which can improve the activity of superoxide dismutase (SOD) in the body, enhance the body's immunity, improve sexual function, anti-aging, anti-fatigue, and anti-tumor And other physiological functions, widely used in medicine, food, cosmetics, chemical industry and other fields. [0003] Squalene is widely found in animals and plants, especially in the liver of deep-sea sharks. In recent years, due to the protection of wild marine animals, and due to the cumbersome process and high extraction...

Claims

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Application Information

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IPC IPC(8): C07C7/152C07C7/10C07C11/21
Inventor 姚忠肖环孙芸张晨曦仲兆祥
Owner NANJING UNIV OF TECH
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