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Method for extracting sialic acid from bird nests by means of crystallization

A sialic acid and crystallization technology, applied in the field of food processing, can solve the problems of cumbersome process and low product yield, and achieve the effects of simplifying extraction steps, simplifying separation and purification steps, and reducing industrial pollution

Inactive Publication Date: 2014-10-01
南京极燕食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patents UN1523031A and CN103060403A respectively announced the method for extracting sialic acid from whey and egg yolk powder, but the process is cumbersome and requires complex processes such as acid hydrolysis / enzyme hydrolysis, ion exchange, concentration, freeze-drying, etc., and the product yield is relatively high. Low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Soak 3kg bird's nest in 45kg deionized water at room temperature for 6 hours, and remove impurities such as bird hair during the soaking process;

[0017] (2) Get the soaked bird's nest, add 30 kg of deionized water with a quality of 10 times the dry weight of the bird's nest, and crush the bird's nest at room temperature with beating equipment;

[0018] (3) Place the beaten bird's nest in a reaction device, and heat at 115°C for 30 minutes to obtain a bird's nest solution;

[0019] (4) centrifuge the heated bird's nest solution at 4500rpm / min for 15min, and get the supernatant;

[0020] (5) Take the supernatant, and freeze-dry at -45°C and 10Pa for 24 hours to obtain the crude sialic acid;

[0021] (6) dissolving the crude sialic acid in ethanol, and filtering to remove alcohol-insoluble impurities;

[0022] (7) Add ethyl acetate in the filtrate, the volume is 0.5 times of ethanol volume, make sialic acid crystals separate out, filter, dry to obtain 170g of siali...

Embodiment 2

[0024] (1) Soak 5kg bird's nest in 100kg deionized water at room temperature for 8 hours, and remove impurities such as bird hair during the soaking process;

[0025] (2) Get the soaked bird's nest, add 75kg of deionized water with a quality of 15 times the dry weight of the bird's nest, and crush the bird's nest at room temperature with beating equipment;

[0026] (3) placing the beaten bird's nest in a reaction device, and heating at 121°C for 40 minutes to obtain a bird's nest solution;

[0027] (4) centrifuge the heated bird's nest solution at 5000rpm / min for 10min, and get the supernatant;

[0028] (5) Take the supernatant, and freeze-dry it at -50°C and 15Pa for 30 hours to obtain the crude sialic acid;

[0029] (6) dissolving the crude sialic acid in ethanol, and filtering to remove ethanol-insoluble impurities;

[0030] (7) Add ethyl acetate in the filtrate, the volume is 2 times of the volume of ethanol, the sialic acid crystals are separated out, filtered, and drie...

Embodiment 3

[0032] (1) Soak 1 kg of swallow cake in deionized water at room temperature for 12 hours, and remove impurities such as swallow hair during the soaking process;

[0033] (2) Take the soaked swallow cake, add 25 kg of deionized water with a quality of 25 times the weight of the swallow biscuit, and crush the swallow cake at room temperature with a beating equipment;

[0034] (3) Place the whipped swallow cake in the reaction device and heat at 125°C for 50min;

[0035] (4) centrifuge the heated swallow cake solution at 5500rpm / min for 5min, and take the supernatant;

[0036] (5) Freeze-dry the supernatant at -55°C and 20 Pa for 48 hours to obtain crude sialic acid;

[0037] (6) dissolving the sialic acid crude product in ethanol, and filtering to remove alcohol-insoluble impurities;

[0038] (7) add ethyl acetate in filtrate, volume is 3 times of ethanol volume, make sialic acid crystal separate out, filter, dry to obtain sialic acid product 54g, purity is 69%, be 100g / In k...

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Abstract

The invention discloses a method for extracting sialic acid from bird nests by means of crystallization. The method comprises the steps of soaking the bird nests with deionized water and removing impurities; then pulping and crushing, heating under a high pressure, centrifuging, drying, dissolving and crystallizing to obtain the sialic acid. According to the method disclosed by the invention, hydrolysis of the glycoproteins of the bird nests is accelerated through serious heating by means of high-pressure heating, and the combined-state sialic acid is converted to a free-state sialic acid; the high-pressure heating avoids the introduction of other ingredients, thereby simplifying the subsequent separation and purification steps; the sialic acid crude product obtained by freeze-drying is prepared into alcohol-saturated solution by virtue of the property that the sialic acid is slightly dissolved in alcohol but proteins and polysaccharides are insoluble in alcohol, and therefore on one hand, the impurities such as alcohol-insoluble proteins and polysaccharides are removed, on the other hand, the solvent polarity is changed by adding a non-polar solvent, and sialic acid crystals are obtained according to the different seperation sequences of the sialic acid and the impurities. According to the method disclosed by the invention, sialic acid extraction steps are simplified, and industrial pollution is reduced.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a method for extracting sialic acid from bird's nest by crystallization. Background technique [0002] Sialic acid, also known as neuraminic acid, widely exists in animal tissues and microorganisms, and is usually located in the key position of the carbohydrate part of the outermost layer of the cell membrane and the secreted carbohydrate complex (glycolipid, glycoprotein and lipopolysaccharide). An important material basis for the diversification of animal structure and function. Sialic acid has physiological effects such as improving infant intelligence and memory, anti-senile dementia, anti-recognition, anti-virus, and improving intestinal absorption of vitamins and minerals. With the further research and understanding of the biological activity and application of sialic acid, products produced from sialic acid have emerged, and the demand for sialic acid ...

Claims

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Application Information

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IPC IPC(8): C07H7/033C07H1/08
Inventor 吴华钟芳李玥徐菲菲殷春燕陈茂深袁玲
Owner 南京极燕食品有限公司
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