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Capillary electrophoresis analysis system utilizing micro-injection pump to drive liquid flows

A micro-injection pump and capillary electrophoresis technology, which is applied in the direction of material analysis, analysis materials, and measuring devices by electromagnetic means, can solve the problem that the reproducibility of capillary electrophoresis separation and determination is not as good as high performance liquid chromatography and gas chromatography, limitations, etc. problems, to achieve the effect of shortening the equilibration time, fast analysis, fast and efficient separation

Inactive Publication Date: 2014-09-17
HANGZHOU NORMAL UNIVERSITY
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Problems solved by technology

[0004] Since the temperature, pH, composition and concentration of the electrolyte solution, the activation state of the inner wall of the capillary and the adsorption of the solute all affect the size of the electroosmotic flow, and there are many factors affecting the injection volume of the capillary electrophoresis, the separation and determination of the capillary electrophoresis are important. The reproducibility is not as good as that of high performance liquid chromatography and gas chromatography, which is limited in practical application

Method used

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  • Capillary electrophoresis analysis system utilizing micro-injection pump to drive liquid flows
  • Capillary electrophoresis analysis system utilizing micro-injection pump to drive liquid flows
  • Capillary electrophoresis analysis system utilizing micro-injection pump to drive liquid flows

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Embodiment 1

[0027] see figure 1 , the capillary electrophoresis analysis system of the micro injection pump driven liquid flow of the present invention, comprises: DC high-voltage power supply 1, nano-liter sampling valve 2 with built-in nano-liter sample loop, micro-injection pump 3, capillary tee 4, separation capillary 5, detection device 6 and an electrophoresis waste liquid bottle 7 equipped with an electrophoresis buffer and a stopper; the capillary electrophoresis analysis system is connected to the first port of the capillary tee 4 by the micro syringe pump 3 and the nanoliter sampling valve 2 , the second port of the capillary tee is connected to the separation capillary 5, and the separation capillary 5 passes through the detection window of the detector 6 and then passes into the liquid of the electrophoresis buffer in the electrophoresis waste liquid bottle 7 that is equipped with the electrophoresis buffer and has a bottle stopper. Next, the third port of the capillary tee is...

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Abstract

The invention discloses a capillary electrophoresis analysis system utilizing a micro-injection pump to drive liquid flows. The capillary electrophoresis analysis system comprises a direct-current high-voltage power supply, a nanoliter sample-injection valve with a built-in nanoliter sample ring, the micro-injection pump, a capillary three-way valve, a separation capillary, a detector and an electrophoresis waste liquid bottle with an electrophoresis buffer solution and a bottle plug. The capillary electrophoresis analysis system utilizes the micro-injection pump to drive liquid flows at a flowing rate of nL / min so that the liquid flows can integrally move in the capillary from a positive pole to a negative pole, and the liquid flows driven by the micro-injection pump replace electroosmotic flows so that all ingredients of the sample are separated because of different electrophoresis speeds. The capillary electrophoresis analysis system has the characteristics of high separation efficiency, fast analysis speed, simple processes and good reappearance.

Description

(1) Technical field [0001] The invention relates to a capillary electrophoresis analysis system, in particular to a capillary electrophoresis analysis system driven by a micro-injection pump and an operating method thereof. (2) Background technology [0002] Since the 1970s, capillary electrophoresis has been widely used in chemistry, biology, environmental science, food, pharmacy, medicine and other fields due to its advantages of high separation efficiency, low reagent consumption, and short analysis time. Zone capillary electrophoresis is the most commonly used separation method in capillary electrophoresis. It has good separation ability for ionic compounds and has been widely used in small molecular weight such as amino acids, drugs, metal ions, and toxic and harmful substances in environmental samples. The separation and analysis of compounds can also be used for the separation of biological macromolecules such as proteins. [0003] The quartz capillary column used in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447
Inventor 程和勇刘金华韩超
Owner HANGZHOU NORMAL UNIVERSITY
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