Manchurian wildginger total polysaccharide extract with cough-relieving activity, and extraction method and application thereof

Abstract

The invention discloses a Manchurian wildginger total polysaccharide extract with cough-relieving activity and a preparation method and application thereof, belonging to the technical field of medicines. The Manchurian wildginger total polysaccharide extract is prepared by subjecting the root and / or tuber of Manchurian wildginger to extraction with a polar solvent, carrying out ethanol precipitation and then carrying out drying under reduced pressure, wherein the yield and the content of the total polysaccharide are 15 wt% and 60 to 80 wt%, respectively. Through a great number of pharmacodynamic tests, it is proved for the first time that the Manchurian wildginger total polysaccharide extract has a substantial cough inhibiting effect which is close to a treatment effect of codeine and shows a better effect on prolonging the incubation period of cough compared with codeine, and it is found out for the first time that the Manchurian wildginger total polysaccharide extract is capable of reducing sensitivity of cough and inhibiting airway inflammations, has good prevention and treatment effects on a variety of cough and can be used for preparation of drugs used for treating diseases related to cough. The Manchurian wildginger total polysaccharide extract has the advantages of definite activity, usage of original plants as experimental materials and low cost.

Description

technical field [0001] The invention belongs to the technical field of medicines, and more specifically relates to an extract of asarum total polysaccharides with antitussive activity, an extraction method and application thereof. Background technique [0002] Due to many factors such as work pressure and air pollution, the incidence of human respiratory diseases remains high, and cough is a common and frequently-occurring disease of the respiratory system, and it is a common complaint in respiratory clinics. Cough itself is a defensive reflex activity produced when the respiratory system is stimulated. It is a beneficial action and is sometimes seen in healthy people. In general, mild and infrequent coughs can be relieved naturally without the use of cough medicine. In addition, the impact of cough on human health is also dialectical, and it is also a pathological response. Long-term frequent or too intense cough will not only increase the suffering of patients, affect res...

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Problems solved by technology

Our previous research found that Asarum volatile oil has obvious antitussive effect on the acute cough guinea pig model stimulated by citric acid, but the antitussive effect on the cough hypersensitive guinea pig model exposed to cigarettes is obviously not as good as the non-volatile component of Asarum total polysaccharide , and large doses have certain side effects

In addition, there are few research reports on the non-volatile components of Asarum. Bell amide Ⅰ, kaempferol-3-O-glucoside, etc. (Cai Shaoqing. Research on the non-volatile chemical components of Asarum. Beijing Medical University Journal. 1996,28(3):228-230; Shuai Lv. Chemical constituents of the underground part (Ⅱ). Journal of Shenyang Pharmaceutical University. 2010, 27(9): 707-710; Wei Qingchun. Isolation and identification of chemical constituents of Hancheng Asarum. Shi Zhen Guo Yi Guo Yao. 2010, 21 (3) :676-677)

The preparation method of asarum polysaccharide has been reported (Li Jingjing. Separation, purification and immune activity of asarum polysaccharide. Journal of Changchun Normal University (Natural Science Edition). 2008,27(1):54-58), but this method It needs to be extracted 3 times, each extraction period is longer (6h), and the preparation process requires centrifugation, so it is not suitable for large-scale industrial production; and the total polysaccharide yield of this preparation method is low (9.56wt%), and the sugar content is also relatively low. Low (48wt%-52wt%); In addition, it has been reported that Asarum polysaccharide, a non-volatile component of Asarum, can promote the proliferation of mouse spleen T and B lymphocytes (Li Jingjing. Separation, purification and immune activity of Asarum polysaccharide. Changchun Normal Journal of Chinese Academy of Sciences (Natural Science Edition). 2008,27(1):54-58), but there is no research report on the antitussive activity of Asarum non-volatile components of Asarum total polysaccharides

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