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A kind of preparation technology of chitin colloid

A preparation technology, chitin technology, applied in the field of chitin colloid preparation technology, can solve the problems of waste of water resources, waste of raw materials, waste of demand, etc., to save electric energy and water resources, small and loose colloidal particles, reduce The effect of production costs

Inactive Publication Date: 2016-05-25
ANHUI POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the following disadvantages: one is that the concentration of the colloid cannot be determined, and an unknown amount of chitin colloid is centrifuged out; the other is that the high concentration of strong inorganic acid has an unfavorable impact on the enzymatic reaction of chitin; The third is that an additional buffer solution needs to be configured during the enzymatic reaction, and the steps are cumbersome and waste raw materials; the fourth is that a large amount of water resources are wasted in the production process, and a large amount of inorganic strong acid waste liquid is produced, causing serious environmental pollution; Powered centrifuges waste power and increase equipment costs and time costs
Therefore, chitin colloids have not been able to be industrialized so far

Method used

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  • A kind of preparation technology of chitin colloid
  • A kind of preparation technology of chitin colloid
  • A kind of preparation technology of chitin colloid

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preparation example Construction

[0023] The preparation of N-acetylglucosamine standard solution and the assay method of standard curve are as follows:

[0024] Accurately weigh 1.0000g of N-acetylglucosamine (pre-dried at 70°C to balance weight), and dilute to 1L with deionized water to obtain N-acetylglucosamine mother liquor with a concentration of 1mg / ml.

[0025] Join as follows:

[0026] N-acetylglucosamine standard curve determination formula

[0027]

[0028] Determination method of standard curve

[0029] Take 1mL of N-acetylglucosamine standard solution No. 0, 1, 2, 3, 4, and 5, 1.5mL of DNS solution (standard of the Ministry of Agriculture) and a colorimetric tube, bathe in boiling water for 8min, set the volume of the colorimetric tube to 25mL, shake The absorbance was measured at 540nm with a UV spectrophotometer. Make a standard curve according to the amount and absorbance of N-acetylglucosamine.

Embodiment 1

[0031] (1), weigh chitin solid and pass through a 100-mesh sieve, and dry to obtain chitin powder;

[0032] (2) Dissolve 1.0000g of chitin powder in 20mL of supersaturated citric acid, heat to 40°C, stir and dissolve with a magnetic stirrer for 12 hours, at 40°C, 250W ultrasonic power for 30 minutes, and then at 40°C Magnetic stirring for 24 hours;

[0033] (3) Slowly add 2mol / L sodium hydroxide solution dropwise to the chitinic acid solution under ultrasonic conditions of 250W power under ultrasonic at 10°C, adjust the pH value to 5.5, and become a colloidal liquid, and continue ultrasonic vibration for 30 Minutes, then stirred at room temperature with a magnetic stirrer for 12 hours.

[0034] Add distilled water or deionized water, and prepare chitin colloid with a concentration of 1.0000% in a 100ml volumetric flask. Fine-tune the pH to 6.0 with a small amount of strong acid or base. Finally, an infrared laser pointer is used to detect whether there is an obvious straigh...

Embodiment 2

[0041] (1), weigh chitin solid and pass through a 100-mesh sieve, and dry to obtain chitin powder;

[0042] (2) Dissolve 1.0000g of chitin powder in 30mL of supersaturated citric acid, heat to 40°C, stir and dissolve with a magnetic stirrer for 12 hours, at 40°C, 250W ultrasonic power for 30 minutes, and then at 40°C Magnetic stirring for 24 hours;

[0043] (3) Slowly add 2mol / L sodium hydroxide solution dropwise to the chitinic acid solution under ultrasonic conditions of 250W power under ultrasonic at 10°C, adjust the pH value to 5.5, and become a colloidal liquid, and continue ultrasonic vibration for 30 Minutes, then stirred at room temperature with a magnetic stirrer for 12 hours.

[0044]Add distilled water or deionized water, and prepare chitin colloid with a concentration of 1.0000% in a 100ml volumetric flask. Use a small amount of strong acid or strong base to fine-tune the pH to 6.0, and finally use an infrared laser pointer to check whether there is an obvious re...

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Abstract

The invention provides a preparation process of a chitin colloid. The process comprises the following steps: drying, crushing and sieving chitin solids to prepare chitin powder; dissolving in an organic acid to prepare a chitin acidic solution; and adjusting the pH to be not greater than 7 by alkaline liquor to prepare the chitin colloid. The process provided by the invention has the main advantages that the chitin colloid prepared in an ultrasonic vibrating environment in an ultrasonic cleaner is small in particle and loose, good in enzyme digestion effect and more suitable for enzymatic reaction; the chitin colloid is not filtered and washed, and the concentration of the chitin colloid can be precise; no buffer solutions are additionally prepared, so that the production cost is lowered; accidental damage to chitin by strong acids needs not to be worried as well as damage to the operators needs not to be worried; centrifugalization and repeated washing are avoided, so that a lot of electric energies and water resource are saved; the process is free from byproducts and environmental pollution and environmentally friendly.

Description

technical field [0001] The invention relates to the field of synthesis, in particular to a preparation process of chitin colloid. Background technique [0002] Chitin (chitin) is also known as chitin, chitin. It is a polysaccharide with the same structure as N-acetylglucosamine polymerized by β linkage. It is widely found in the shells of crustaceans, the carapaces of insects and the cell walls of fungi, and also exists in some green algae. Physical properties: Appearance is off-white amorphous substance, odorless and tasteless. Solubility: Soluble in dimethylacetamide containing 8% lithium chloride or concentrated hydrochloric acid; insoluble in water, dilute acid, alkali, ethanol or other organic solvents. Hydrolysis: Completely hydrolyzed into chitosan (2-glucosamine) with acid. Product use: Chitin is an important raw material for preparing chitosan and glucosamine series products. Chitin and its derivatives have important uses in medicine, chemical industry, health ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/11C08L5/08
Inventor 胡俊张庆庆郑天柱蒋汶谭胜徐得磊刘亚军刘文
Owner ANHUI POLYTECHNIC UNIV
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