Method for inducing nematode-trapping fungi to produce capturing devices through amino acid
A technology of preying on nematode fungi and preying organs, applied in the field of applied microbiology, can solve the problems of undiscovered public reports, etc., and achieve strong controllability and good repeatability
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Embodiment 1
[0046] Example 1: Valine (Val) induces the predatory nematode fungus Arthrobush oligosporum to produce predatory organs
[0047] The CMA medium was prepared according to the method described in the Summary of the Invention, and then distributed into 250mL Erlenmeyer flasks (40mL / bottle), sealed with gauze and newspaper, and sterilized at 121°C for 20 minutes. The sterilized culture medium was left at room temperature for 2 days to allow the water remaining on the bottle wall and the surface of the culture medium to dissipate as much as possible. Under sterile conditions, use a puncher to inoculate Arthrophyllum oligospora blocks of the same size on the above CMA solid medium, and culture them in a constant temperature incubator at 28°C for 14 days to obtain a large number of spores.
[0048] According to the spore collection method described in the summary of the invention, collect the spores of Arthropodium oligospora, adjust the concentration of the spore suspension to about...
Embodiment 2
[0049] Example 2: Valine (Val) induces the nematode-predating fungus Arthrobus annuli to produce predatory organs
[0050] The CMA medium was prepared according to the method described in the Summary of the Invention, and then distributed into 250mL Erlenmeyer flasks (40mL / bottle), sealed with gauze and newspaper, and sterilized at 121°C for 20 minutes. The sterilized culture medium was left at room temperature for 2 days to allow the water remaining on the bottle wall and the surface of the culture medium to dissipate as much as possible. Under sterile conditions, use a puncher to inoculate Arthrophyllum spp. blocks of the same size on the above-mentioned CMA solid medium, and culture them in a constant temperature incubator at 28°C for 14 days to obtain a large number of spores.
[0051] According to the method for collecting spores described in the summary of the invention, collect the spores of Arthrophyllum spp., adjust the concentration of the spore suspension to about 1...
Embodiment 3
[0052] Example 3: Leucine (Leu) induces the nematode-predating fungus Arthrophyllum oligosporum to produce predatory organs
[0053] The CMA medium was prepared according to the method described in the Summary of the Invention, and then distributed into 250mL Erlenmeyer flasks (40mL / bottle), sealed with gauze and newspaper, and sterilized at 121°C for 20 minutes. The sterilized culture medium was left at room temperature for 2 days to allow the water remaining on the bottle wall and the surface of the culture medium to dissipate as much as possible. Under sterile conditions, use a puncher to inoculate Arthrophyllum oligospora blocks of the same size on the above CMA solid medium, and culture them in a constant temperature incubator at 28°C for 14 days to obtain a large number of spores.
[0054] According to the spore collection method described in the summary of the invention part, collect the spores of Arthropodium oligospora, adjust the concentration of the spore suspension...
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