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Method for fermentation production of micro molecular weight dextran

A dextran and micro-molecular-weight technology, applied in the field of fermentation, can solve problems such as low sucrose conversion efficiency, insufficient material exchange, and large molecular weight of the product, and achieve the effects of favorable fermentation process control, short fermentation cycle, and high fermentation efficiency

Inactive Publication Date: 2014-08-20
GUANGDONG PROVINCIAL BIOENGINEERING INST (GUANGZHOU SUGARCANE IND RES INST)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the process of microbial fermentation to produce dextran has a high viscosity and insufficient material exchange, resulting in low conversion efficiency of sucrose; and the product has a large molecular weight and cannot be used directly. Usually, it needs to be subjected to acid hydrolysis followed by ethanol fractionation to obtain dextran with different molecular weights. Take clinically required dextran

Method used

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  • Method for fermentation production of micro molecular weight dextran
  • Method for fermentation production of micro molecular weight dextran
  • Method for fermentation production of micro molecular weight dextran

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The composition of the fermentation medium is Na 2 HPO 4 1.4 g / L, KH 2 PO 4 0.3 g / L, peptone 2 g / L, sucrose 130 g / L, pH 6.4, sterilized at 121°C for 20 min.

[0056] Fermentation culture conditions:

[0057] Inoculate the Leuconostoc enterococcus bacteria liquid with 10% inoculation amount in a 5L fermenter with 2.7 L fermentation medium for fermentation, the temperature is 26°C, and the rotation speed is 50rpm in the early stage of fermentation. Gradually increase the rotation speed to 100rpm, adjust the pH to 6.5 through 1mol / L NaOH solution, and continuously inject a small amount of sterile air;

[0058] After 16 hours of fermentation, the bacteria enter the logarithmic growth phase, add dextran hydrolase to the fermentation system to make the final concentration 0.2U / mL, and at the same time add 240g of sucrose with a concentration of 600g / L at a flow rate of 45g / h;

[0059] After feeding, add dextran hydrolase to the fermenter again to make the final conce...

Embodiment 2

[0062] The composition of the fermentation medium is Na 2 HPO 4 1.4 g / L, KH 2 PO 4 0.3 g / L, peptone 2 g / L, sucrose 130 g / L, pH 6.4, sterilized at 121°C for 20 min.

[0063] Fermentation culture conditions:

[0064] Inoculate the Leuconostoc enterococcus bacteria liquid with 10% inoculation amount in a 5L fermenter with 2.7 L fermentation medium for fermentation, the temperature is 26°C, and the rotation speed is 50rpm in the early stage of fermentation. Gradually increase the rotation speed to 100rpm, adjust the pH to 6.5 through 1mol / L NaOH solution, and continuously inject a small amount of sterile air;

[0065] After 16 hours of fermentation, the bacteria enter the logarithmic growth phase, add dextran hydrolase with a final concentration of 0.2U / mL to the fermentation system, and simultaneously add 360g of sucrose with a concentration of 600g / L at a flow rate of 45g / h;

[0066] After feeding, dextran hydrolase was added to the fermenter again to make the final con...

Embodiment 3

[0069] The composition of the fermentation medium is Na 2 HPO 4 1.4 g / L, KH 2 PO 4 0.3 g / L, peptone 2 g / L, sucrose 130 g / L, pH 6.4, sterilized at 121°C for 20 min.

[0070] Fermentation culture conditions:

[0071] Inoculate the Leuconostoc enterococcus bacteria liquid with 10% inoculation amount in a 5L fermenter with 2.7 L fermentation medium for fermentation, the temperature is 26°C, and the rotation speed is 50rpm in the early stage of fermentation. Gradually increase the rotation speed to 100rpm, adjust the pH to 6.5 through 1mol / L NaOH solution, and continuously inject a small amount of sterile air;

[0072] After 16 hours of fermentation, the bacteria enter the logarithmic growth phase, add dextran hydrolase with a final concentration of 0.2U / mL to the fermentation system, and simultaneously add 360g of sucrose with a concentration of 600g / L at a flow rate of 45g / h;

[0073] After feeding, add dextran hydrolase to the fermenter again to make the final concentra...

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Abstract

The present invention discloses a method for fermentation production of micro molecular weight dextran. According to the method, dextran hydrolase is added to a fermentation system at a bacterial growth logarithmic phase while a sucrose solution is subjected to fed batch so as to effectively improve the conversion efficiency of the sucrose, wherein conversion of 390 g of the sucrose in 24 h in the traditional process is increased to conversion of 750 g of the sucrose in 36 h; and after completing the fed batch, dextran hydrolase is added to the fermentation tank, and the molecular weight (less than 20000 Da) of the micro molecule dextran product can be controlled according to requirements, such that the production of the micro molecular weight dextran through the one-step method can be achieved, acid hydrolysis and alcohol fractional precipitation are not required, and the production process is simplified. According to the present invention, the sucrose conversion efficiency is significantly increased, the effective control effect on the molecular weight of the micro molecule dextran is significant, and the method of the present invention is the effective method for fermentation production of micro molecular weight dextran.

Description

technical field [0001] The invention relates to a fermentation method, in particular to a method for producing micromolecular weight dextran by fermentation. Background technique [0002] Dextran (dextran), also known as glucan, is a high molecular polymer polymerized by several glucose. It is the earliest microbial polysaccharide discovered and the first microbial exopolysaccharide approved by the US FDA for food. It is a microbial polysaccharide that was used as plasma earlier. [0003] Dextran-70 is currently recognized as one of the excellent plasma substitutes and has been widely used in clinical medicine; Dextran-40 and Dextran-20 have the effect of improving microcirculation and replenishing a certain blood volume. In addition to being used as a plasma substitute, micro-molecular-weight dextran (molecular weight below 20000Da) can also form a series of extremely versatile derivatives, such as iron dextran (molecular weight of dextran is 5000-7500Da), dextran-doxorubi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/08C12R1/01
Inventor 梁达奉曾练强张九花蚁细苗黎志德常国炜吴兆鹏李雨虹黄曾慰柳颖
Owner GUANGDONG PROVINCIAL BIOENGINEERING INST (GUANGZHOU SUGARCANE IND RES INST)
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