Diagnostic kit for mycobacterium tuberculosis dormant infection

Abstract

The invention discloses a diagnostic kit for mycobacterium tuberculosis dormant infection. The kit can specifically recognize at least one of cell factors Eotaxin-2, MIG, Eotaxin, MCSF or 1L-12p70, can quantitatively analyze the recognized cell factors and specifically recognize antigens 38-KDa, 32-KDa and 14-16-KDa and specific IgG antibody of Ag85B. The selected five cell factors have remarkable expression difference in uninfected population and mycobacteria dormant infection patients, are used as markers for detecting the mycobacteria dormant infection patients, can well differentiate dormant infection persons and uninfected persons, and has high expression quantity and high diagnostic sensitivity; besides, the kit is combined with the specific antibody for detecting four antigens, the diagnosis accuracy and the diagnosis sensitivity are further improved; the kit is directly used for detecting markers in serum, is convenient and rapid to operate and is suitable for popularization and application.

Description

technical field [0001] The invention belongs to the field of immunodiagnosis, and in particular relates to a diagnostic kit for latent infection of mycobacterium tuberculosis. Background technique [0002] Tuberculosis is an ancient disease that coexists in the history of human development, and it is still a global health problem. It is estimated that one-third of the world's population has been infected with Mycobacterium tuberculosis, and 10% of these infected people will progressed to active tuberculosis. There are 10 million new cases and more than 3 million deaths each year. In view of the threat to human life and health, medical workers in the world have not relaxed their research on tuberculosis. The onset process of tuberculosis is generally from healthy people to infected people, and then develops into active tuberculosis after a incubation period of varying lengths. Epidemiology shows that one-third of the global population is infected with Mycobacterium tubercu...

AI Technical Summary

Problems solved by technology

PPD is currently the most widely used method, but it has disadvantages such as long time for result interpretation, strong subjectivity of result interpretation, and poor sensitivity.

The IFN-γ release experiment takes two days to report, and the detection efficiency is higher than that of PPD, but its operation is cumbersome, including multiple steps such as blood collection, cell separation, cell stimulation culture, immunological reaction, and computer detection, and it requires dedicated It is unrealistic to spread the expensive equipment to grassroots organizations

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