Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Quantitative determination method of immune chromatography test strip

A quantitative detection method and the technology of immunochromatographic test paper, which are applied in the field of dry chemical detection, can solve the problems of increasing the labor complexity of clinical testing staff, and achieve the effect of increasing the linear detection range and improving accuracy

Inactive Publication Date: 2014-07-30
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
View PDF6 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In clinical applications, operators have to manually dilute, which increases the labor complexity of clinical testing staff

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Quantitative determination method of immune chromatography test strip
  • Quantitative determination method of immune chromatography test strip
  • Quantitative determination method of immune chromatography test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A method for quantitatively detecting C-reactive protein is provided, comprising the steps of:

[0049] 1. Structural design of immunochromatographic test strips

[0050] Four protein bands are immobilized on the reaction membrane of the test strip, which are an internal reference line immobilized with 0.2 μg rabbit IgG, the first detection line immobilized with 0.4 μg CRP monoclonal antibody a, and immobilized with 0.4 μg CRP monoclonal antibody a. A second test line for antibody a, an excess antigen test line immobilized with 0.5 μg goat anti-mouse IgG.

[0051] 2. Detection buffer design

[0052] The detection buffer contained 0.2 μg of cy5 fluorescently labeled goat anti-rabbit IgG and 1 μg of cy5-labeled mouse CRP monoclonal antibody.

[0053] 3. Quantitative detection algorithm of test strips

[0054] Take 10 serum samples of standard concentration, add them to the detection buffer, add them dropwise to the test strips and react for a period of time, and cooper...

Embodiment 2

[0062] A quantitative detection method for rapid detection of parathion-methyl is provided, comprising the steps of:

[0063] 1. Structural design of the test strip

[0064] Four bands are fixed on the reaction membrane of the test strip, which are an internal reference line immobilized with 1 μg rabbit IgG, the first detection line immobilized with 0.5 μg methyl parathion artificial antigen, and immobilized with 0.5 μg methyl parathion A second test line for phospho-artificial antigen, an excess antigen test line immobilized with 0.5 μg goat anti-mouse IgG.

[0065] 2. Detection buffer design

[0066] The detection buffer contained 1 μg of cy5 fluorescently labeled goat anti-rabbit IgG and 1 μg of cy5-labeled mouse methyl parathion monoclonal antibody.

[0067] 3. Quantitative detection algorithm of test strips

[0068] Take 7 standard concentrations of methyl parathion solution, add it to the detection buffer, drop it on the test strip and react for a period of time, and ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a quantitative determination method of an immune chromatography test strip. The quantitative determination method comprises the following steps: drawing a standard curve; dropwise adding a liquid to be detected to the immune chromatography test strip which comprises an internal reference line, at least one detection line and an excessive antigen detection line, detecting, and reading a detection signal capable of reflecting the intensity of antigen-antibody reaction to obtain a reading result; carrying out linear regression on the reading result of the nth detection line by utilizing the nth standard curve when the detection signals of the n-1 detection lines of at least one detection line exceed a threshold value according to the reading results, and calculating to obtain the concentration of the liquid to be detected. By adopting the mode, the quantitative detection method disclosed by the invention can solve the problem that the low-concentration measurement and the high-concentration measurement are simultaneously carried out, and can be used for the quantitative detection on biological macromolecules or micromolecules, such as serum markers, microbe antigens, virus particles and illicit drugs; the linear detection range of the test strip is enlarged, the detection accuracy is improved, and the accurate quantitative detection on a target detection object is realized.

Description

technical field [0001] The invention relates to the field of dry chemical detection, in particular to a quantitative detection method of an immunochromatographic test strip. Background technique [0002] Immunochromatography uses a nitrocellulose membrane as a support for reactants, and uses the pore effect of the membrane to react. The technology can be divided into vertical diffusion and horizontal diffusion. The dot gold hybridization method of vertical diffusion was born in the early stage, but it was not popularized and used as a mainstream reagent because of its complicated operation. With the development of membrane technology, lateral flow and lateral diffusion test strips have shown good advantages and have been greatly promoted. The most widely used colloidal gold rapid diagnostic test paper in lateral flow immunochromatography is used in the detection of early pregnancy (HCG). Due to its convenience, speed, and high specificity, it is especially suitable for imm...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/558
CPCG01N33/54373
Inventor 尹焕才
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com