Peak conversion method of three-dimensional spectroscopic data under different photomultiplier tube voltages

A photomultiplier tube and spectral data technology, applied in the field of peak conversion, can solve problems such as the difficulty in accurately determining the specific position of the Raman peak, the uncalibrated fluorescence signal, and the dependence of the fluorescence signal intensity, etc., to achieve convenient and fast accuracy, convenient comparison, The effect of high accuracy

Inactive Publication Date: 2014-07-30
SHANGHAI UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] For fluorescence data, there are generally related calibration steps before analysis. The first is to correct the spectral properties of the instrument. characteristics, not the real spectrum of the fluorescent substance itself; followed by the correction of the absorption properties of the sample (usually an internal filtering effect); but through the first two steps, the intensity of the fluorescent signal is not corrected, in the same case, the The position of the peak will be affected by the photomultiplier tube of the instrument, and the intensity of the fluorescent signal is also very dependent on the photomultiplier tube voltage
However, this last critical correction step is often overlooked
[0004] At ...

Method used

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  • Peak conversion method of three-dimensional spectroscopic data under different photomultiplier tube voltages
  • Peak conversion method of three-dimensional spectroscopic data under different photomultiplier tube voltages
  • Peak conversion method of three-dimensional spectroscopic data under different photomultiplier tube voltages

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Embodiment 1

[0026] see Figure 1~Figure 4 , the peak conversion method of three-dimensional fluorescence spectrum data under different photomultiplier tube voltages, including two steps of numerical correction and peak conversion:

[0027] Described value correction step comprises the steps:

[0028] (1) Set the three-dimensional fluorescence measurement conditions, in which the voltage of the photomultiplier tube is set to 500V, 650V, 800V, and 900V in sequence, and the excitation-emission matrix spectrum of deionized water is measured to obtain the data of excitation wavelength-emission wavelength-fluorescence intensity;

[0029] (2) Use the special reagent for fluorescence analysis, quinine sulfate solution, to perform linear fitting on the peak value within the corresponding wavelength range under the corresponding voltage and the data obtained in the step (1).

[0030] Described peak conversion step comprises the following steps:

[0031] (3) Linearly fit the peak value of the samp...

Embodiment 2

[0034] This embodiment is basically the same as Embodiment 1, and the special feature is: according to the peak value of the three-dimensional fluorescence spectrum of the solution to be measured under different voltages and the Rayleigh scattering value of deionized water at the excitation wavelength Ex=emission wavelength Em=350nm at the corresponding voltage The linear fitting results can judge the accuracy of the peak value of the three-dimensional fluorescence spectrum of the sample under different voltages, and use the obtained linear equation to correct the value. The peak data of the three-dimensional spectral data under different photomultiplier tube voltages can directly read the measured value of the three-dimensional fluorescence spectral data without additional calculation, which is convenient, fast and highly accurate.

Embodiment 3

[0036] In this experiment, the concentration of quinine sulfate solution, a special reagent for fluorescence analysis, was 1×10 -7 mol / L, the samples used were water samples from water plants, and three-dimensional fluorescence excitation-emission spectroscopy (3DEEM) was measured using a highly sensitive fluorescence spectrophotometer with a 10mm quartz fluorescence sample cell (Shanghai Lenslight F97XP / F97Pro / F97, China) . Excitation light source is 150W xenon lamp, bandwidth: excitation bandwidth is 10nm, emission bandwidth is 10nm; response time: automatic; scanning speed: 15000nm / min; excitation wavelength range is Ex=200-700nm (interval 5nm), emission wavelength range is Em= 250-750nm (interval 5nm), the experiment is divided into four groups according to different photomultiplier tube voltages, namely: 500V, 650V, 800V and 900V, each group first measures the deionized water, and then performs the determination of the sample. The temperature is constant. The obtained t...

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Abstract

The invention discloses a peak conversion method of three-dimensional fluorescence spectra under different photomultiplier tube voltages. The method includes two steps of numerical correction and peak conversion. Numerical correction includes: setting the determination conditions; respectively determining three-dimensional three-dimensional fluorescence spectra of deionized water and a calibration reagent quinine sulfate; reading a Rayleigh scattering value in the three-dimensional fluorescence spectrum of deionized water at the excitation wavelength Ex equal to emission wavelength Em of 350 nm and the peak of the quinine sulfate solution under the same conditions (PMT voltage, excitation-emission scanned area); and conducting linear fitting of the two values, and determining the accuracy of the three-dimensional fluorescence data according to the results of the linear fitting. Peak conversion includes: conducting linear fitting of the peak of a sample under a known voltage and specific wavelength range and the Rayleigh scattering value in the three-dimensional fluorescence spectrum of deionized water at the excitation wavelength Ex equal to emission wavelength Em of 350 nm under corresponding voltage; and converting the peak of the sample into corresponding peaks under different voltages according to the obtained linear equation.

Description

technical field [0001] The invention relates to a method for peak conversion of three-dimensional spectral data under different voltages of photomultiplier tubes. The method includes two steps of numerical correction and peak conversion. The scattering value of water is skillfully used to quantitatively evaluate the power of different photoelectric multiplier tubes. innovative. The conversion of the peak value of the three-dimensional fluorescence spectrum under different photomultiplier tube voltages can be realized quickly, accurately and efficiently. Background technique [0002] Three-dimensional fluorescence spectroscopy (also known as total luminescence spectrum or excitation-emission diagram) is a new analysis technology developed on the basis of fluorescence spectroscopy analysis in the 1980s. It can not only improve measurement sensitivity and selectivity to molecular structure, Moreover, it can display the fluorescence information of the sample more comprehensivel...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 黄鑫刘佳卢宁刘爽刘茵张东黄怡
Owner SHANGHAI UNIV
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