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Method for extracting active collagen peptide with low molecular weight from chicken skin

A collagen peptide and low molecular weight technology, which is applied in the deep processing of chicken skin, can solve the problems of low biological activity, no extraction of low molecular weight active collagen peptide, etc., and achieve the effect of optimizing process steps, increasing yield and improving activity

Inactive Publication Date: 2014-07-30
GUILIN JUNGONG BIOCHEM TECH DEVCO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the molecular weight of domestically produced collagen peptides is mostly above 2000 Daltons, and their biological activity is very low.
[0005] After a patent search, no relevant literature on the method of extracting low-molecular-weight active collagen peptides from chicken skin has been found

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Fat removal: use fresh chicken skin as raw material, cut into pieces and wash, and use 18 times the weight of raw material with a mass concentration of 1.2% H 2 O 2 Stir and soak the 0.01mol / L NaOH solution at 18°C ​​for 4 hours, wash with water until it is neutral, and drain to obtain fat-free chicken skin;

[0021] (2) Preparation of collagen: add deionized water 10 times the weight of the raw material to the fat-free chicken skin, heat up to 45°C, add 1‰ of the raw material quality aminopeptidase and carboxypeptidase mixed protease, carry out enzymatic hydrolysis, and the chicken skin will disintegrate. Loose collagen;

[0022] (3) Preparation of low molecular weight active collagen peptide: Add 3‰ endoprotease of raw material weight to loose collagen, further enzymolysis, after enzymolysis is complete, heat up to 90℃, keep for 30min, inactivate the enzyme, and let it stand. The clear liquid is separated, activated carbon is added, decolorized and filtered, and then f...

Embodiment 2

[0024] (1) Fat removal: use fresh chicken skin as raw material, cut into pieces and wash, and use 19 times the weight of the raw material with a mass concentration of 1.1% H 2 O 2 Stir and soak the 0.01mol / L NaOH solution at 19°C for 4 hours, wash with water until it is neutral, and drain to obtain fat-free chicken skin;

[0025] (2) Preparation of collagen: add deionized water 9 times the weight of the raw material to the fat-free chicken skin, heat up to 43°C, add 2‰ of the raw material quality aminopeptidase and carboxypeptidase mixed protease, carry out enzymatic hydrolysis, and the chicken skin will disintegrate. Loose collagen;

[0026] (3) Preparation of low-molecular-weight active collagen peptide: Add 1‰ endoprotease of raw material weight to loose collagen, and further enzymatic hydrolysis. After enzymatic hydrolysis is complete, the temperature is raised to 95°C, kept for 25 minutes, the enzyme is inactivated, and left to stand. The clear liquid is separated, activated c...

Embodiment 3

[0028] (1) Fat removal: use fresh chicken skin as raw material, cut into pieces and wash, use 20 times the weight of the raw material with a mass concentration of 1.0% H 2 O 2 Stir and soak the 0.01mol / L NaOH solution at 20°C for 4h, wash with water until it is neutral, and drain to obtain fat-free chicken skin;

[0029] (2) Preparation of collagen: Add 7 times the amount of deionized water of the raw material weight to the fat-free chicken skin, heat up to 38°C, add 3‰ of the raw material quality aminopeptidase and carboxypeptidase mixed protease, carry out enzymatic hydrolysis, and the chicken skin will disintegrate. Loose collagen;

[0030] (3) Preparation of low-molecular-weight active collagen peptide: Add 2‰ endoprotease of raw material weight to loose collagen, and further enzymatic hydrolysis. After enzymatic hydrolysis is complete, the temperature is raised to 100°C, kept for 15 minutes, the enzyme is inactivated, and left to stand. The clear liquid is separated, activated...

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Abstract

The invention discloses a method for extracting an active collagen peptide with low molecular weight from chicken skin. The method comprises the following steps: (1) degreasing: with fresh chicken skin as a raw material, shearing, cleaning, adding an NaOH solution of H2O2 of which the mass concentration is 0.8-1.2%, soaking, washing with clear water till being neutral, and draining off to obtain degreased chicken skin; (2) preparation of collagen: adding deionized water to the degreased chicken skin, heating and adding compound protease I for enzymolysis so that the chicken skin is disintegrated into scattered collagen; (3) preparation of the active collagen peptide with low molecular weight: adding compound protease II to the scattered collagen, carrying out further enzymolysis, heating after complete enzymolysis, inactivating the enzyme, standing, separating out clear liquid, adding activated carbon, filtering after destaining, filtering and concentrating by using an ultra-filtration device, and drying concentrated liquid to obtain the active collagen peptide with low molecular weight. The processing steps are optimized, the productivity is improved, the yield of collagen peptide is improved, and the activity of the collagen peptide is improved.

Description

Technical field [0001] The invention relates to the technical field of deep processing of chicken skin, in particular to a method for extracting low molecular weight active collagen peptides from chicken skin. Background technique [0002] Chicken skin is a raw material rich in animal connective tissue and has good cosmetic effects. It is rich in collagen and can smooth the skin and remove wrinkles. The effect is beyond comparison with other things. Collagen has a unique three-strand super-helical structure. The three chains are parallel to each other and are connected by hydrogen bonds between the chains. It has very stable properties. It is difficult to decompose it under general processing temperature and short-term heating, so it is difficult to be absorbed by the body and eat directly. The utilization rate is low; if the collagen is hydrolyzed into peptides, the digestion and absorption rate is almost 100% low, and the physiological functions of its nutrients will also be si...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 周冠旻
Owner GUILIN JUNGONG BIOCHEM TECH DEVCO
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