Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting active components of yeast based on high voltage pulse discharge technology

A high-voltage pulse discharge and active component technology, which is applied in the field of extracting active components, can solve problems such as unfavorable industrial production, thermal reduction of protein and nucleic acid biological activity, and cumbersome soil treatment, so as to achieve suitable promotion and application, and improve the extraction rate. , the effect of simple process

Inactive Publication Date: 2014-07-30
SOUTH CHINA UNIV OF TECH
View PDF4 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] (1) The traditional physical wall-breaking method needs to consume a lot of energy, and the heat generated reduces the biological activity of proteins and nucleic acids
[0004] (2) Chemical treatment can easily cause denaturation of proteins and nucleic acids, and the follow-up treatment is cumbersome
[0005] (3) biological wall breaking method, long reaction time, high cost, unfavorable for industrialized production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting active components of yeast based on high voltage pulse discharge technology
  • Method for extracting active components of yeast based on high voltage pulse discharge technology
  • Method for extracting active components of yeast based on high voltage pulse discharge technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] figure 1 The high-voltage pulse discharge treatment chamber used in this embodiment includes a high-voltage pulse discharge power supply 1, a ground wire 2, a ground electrode 3, a connecting plate 4, a flat plate electrode 5, a multi-pin electrode 6, an insulator 7, a treatment chamber 8, and a sample inlet 9 , Vent 10.

[0027] The yeast active component extraction method based on high-voltage pulse discharge technology of the present embodiment, the steps are as follows: put 10g beer dry yeast (protein content 48-50%, nucleic acid content 6%~9%) in 35 Rehydrate in warm water at ℃ for 15 minutes to make a yeast suspension. Move the prepared beer yeast suspension through the sample inlet into the high-voltage pulse discharge treatment chamber for treatment. Adjust the multi-needle electrodes so that the distance between the multi-needle electrodes and the plate electrodes is kept at 10 cm. The pulse discharge voltage is 10kV, the frequency is 50Hz, the pulse width is ...

Embodiment 2

[0037] The method for extracting yeast active components based on high-voltage pulse discharge technology in this embodiment, the steps are as follows:

[0038] Put 20g of beer dry yeast (48-50% protein content, 6%-9% nucleic acid content) at a ratio of 1:20 in warm water at 38°C for 20 minutes to make a yeast suspension, and prepare the beer yeast suspension Move the samples into the high-voltage pulse discharge treatment chamber through the sample inlet for treatment, and adjust the multi-needle electrodes so that the distance between the multi-needle electrodes and the plate electrodes is kept at 40 cm. The pulse discharge voltage is 40kV, the frequency is 200Hz, the pulse width is 100μs, the pulse number is 1000 times, and the pulse electric treatment time is 20s. The extract rich in active components is obtained, centrifuged at a speed of 4000 r / min for 10 min with a centrifuge, and protein and nucleic acid are obtained in the supernatant. The isoelectric point precipita...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for extracting active components of yeast based on a high voltage pulse discharge technology. The method for extracting the active components of the yeast based on the high voltage pulse discharge technology comprises the following steps: (1) activating the yeast, so that a yeast suspension liquid is obtained; (2) placing the yeast suspension liquid in a high voltage pulse electro discharge treatment chamber for carrying out wall breaking treatment, and then carrying out centrifugation, so that an extracting solution rich in the active components of the yeast is obtained. The method for extracting the active components of the yeast based on the high voltage pulse discharge technology has the advantages that high voltage pulse discharge yeast cell wall breaking rate and active component extraction rate are increased, a technology is simple and extraction rate is high, so that the method for extracting the active components of the yeast based on the high voltage pulse discharge technology is applicable to popularization and application.

Description

technical field [0001] The invention relates to the technical field of extracting active components from yeast, in particular to a method for extracting active components of yeast based on high-voltage pulse discharge technology. Background technique [0002] Beer waste yeast is one of the main by-products in beer production. For every 100 tons of beer produced, 1.5 to 2.0 tons of waste yeast are produced. The annual production of beer waste yeast in my country's beer industry reaches 600,000 to 800,000 tons. At present, most of the waste beer yeast is used as fertilizer and feed, and the utilization rate is very low. Some enterprises directly discharge the waste yeast into beer wastewater as waste, which increases the processing load and difficulty of beer wastewater and causes a lot of waste. Yeast cells contain extremely rich nutrients, and the extraction of these functional active substances is of great significance for the resource utilization of beer waste yeast. Stud...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K1/14C12N15/10
Inventor 刘丹曾新安孙大文王启军
Owner SOUTH CHINA UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com