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Nano-particle for inhibiting aggregation of alpha-beta and metal ions induced alpha-beta, as well as preparation and application

A technology of nanoparticles and metal ions, which is applied in the direction of active ingredients of heavy metals, medical preparations of non-active ingredients, active ingredients of sulfur/selenium/tellurium, etc., can solve the problems of non-selectivity, toxic and side effects of chelation, and achieve improved Biocompatibility, simple method, and the effect of inhibiting Aβ polypeptide aggregation

Inactive Publication Date: 2014-07-30
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most metal chelating agents cannot penetrate the blood-brain barrier. In addition, metal chelating agents are not selective for the chelation of metal ions, which will chelate some necessary metal ions in the body and cause toxic side effects

Method used

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  • Nano-particle for inhibiting aggregation of alpha-beta and metal ions induced alpha-beta, as well as preparation and application
  • Nano-particle for inhibiting aggregation of alpha-beta and metal ions induced alpha-beta, as well as preparation and application
  • Nano-particle for inhibiting aggregation of alpha-beta and metal ions induced alpha-beta, as well as preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: the preparation of nano-selenium, nano-ruthenium and composite nano-selenium / ruthenium modified by arginine

[0039] (1) Weigh 0.1729g of sodium selenite, dilute to 10mL with distilled water to prepare a 0.1mol / L storage solution; at the same time weigh 0.0435g of arginine, dilute to 10mL with distilled water to prepare a 0.025mol / L storage solution ; Weigh 0.0259g of ruthenium trichloride, and make it to 5mL with distilled water to prepare a 0.025mol / L storage solution.

[0040] (2) Mix 0.2mL sodium selenite stock solution with different proportions of arginine, gradually add different proportions of sodium borohydride dropwise at 400rpm / min, stop stirring after the color changes to brick red, indicating that the The molar ratios of nano-selenium, sodium selenite to arginine or sodium borohydride are 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, respectively. Among them, the molar ratio of sodium selenite to arginine in arginine-modified nano-selenium was 1:4, which was...

Embodiment 2

[0044] Embodiment 2: the preparation of nano-selenium, nano-ruthenium and composite nano-selenium / ruthenium modified by gallic acid

[0045] (1) Weigh 0.1729g of sodium selenite, dilute to 10mL with distilled water to prepare a 0.1mol / L storage solution; at the same time weigh 0.0425g of gallic acid, dilute to 10mL with distilled water to prepare a 0.025mol / L storage solution; Weigh 0.0259 g of ruthenium trichloride, and dilute to 5 mL with distilled water to prepare a 0.025 mol / L storage solution.

[0046] (2) Take 0.2mL sodium selenite stock solution, gradually add different proportions of gallic acid dropwise under the condition of 400rpm / min, stop stirring after the color changes to brick red, indicating that nano-selenium has been reduced, and finally sodium selenite The molar ratios to gallic acid are 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, respectively. Among them, the molar ratio of sodium selenite to gallic acid in gallic acid-modified nano-selenium was the most stable. The a...

Embodiment 3

[0050] Example 3: Y-modified nano-selenium, nano-ruthenium and composite nano-selenium / ruthenium combined with Aβ1-40 polypeptide

[0051] First take 30μM Aβ 40 (purchased from Shanghai GL Biochem Ltd.) Incubated at 37°C for 3 days to form fibrous aggregates, and then added 60 μg / mL of Rhodamine B-labeled nanoparticles prepared in Example 1 and Example 2 to the sample Particles were incubated for another 6 h. Take 10 μL of the above solution and drop it on the glass slide whose surface has been treated with positive charge. After the sample is dried, observe it under a confocal laser microscope. The results show that the nano-selenium, nano-ruthenium and composite nano-selenium / ruthenium modified respectively by arginine, histidine, glucose, sucrose, vitamin C and gallic acid prepared by the present invention can be combined with Aβ 40 Polypeptide binding, wherein the amount of nano-ruthenium and composite nano-selenium / ruthenium binding to polypeptides is more than that of ...

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Abstract

The invention belongs to the technical field of nano-medicaments, and discloses a nano-particle for inhibiting aggregation of alpha-beta and metal ion induced alpha-beta, as well as a preparation method and application thereof. The nano-particle is Y-modified nano-ruthenium and / or selenium nano-particle, and Y is at least one of arginine, histidine, glucose, saccharose, vitamin C and gallic acid; the Y-modified nano-particle comprises nano-ruthenium, nano-selenium and composite nano-ruthenium-selenium, can be used for inhibiting spontaneous aggregation of alpha-beta, has the effect of inhibiting aggregation of metal ion induced alpha-beta polypeptide, and can be applied to preparation of anti-alzheimer disease medicaments. According to the nano-particle modified by vitamin C and gallic acid, the vitamin C and the gallic acid are directly used as a reducing agent and a modifying agent, other auxiliary reagents need not to be added in the preparation process, the product system and the operation are simple, the method is simple and easy, and the product can be directly stored and used.

Description

technical field [0001] The invention belongs to the technical field of nanomedicine, and in particular relates to a nano particle for inhibiting Aβ and metal ion-induced Aβ aggregation, a preparation method and application thereof. Background technique [0002] Alzheimer's disease (AD) is a progressive neurodegenerative disease characterized by memory and cognitive dysfunction, also known as senile dementia. AD is more common in the elderly, and the prevalence of AD in people over 60 years old is about 5-10%, while the prevalence of AD in people over 85 years old increases sharply, as high as 40-50%. With the aging of the population and the lack of effective means of prediction and treatment, the number of AD patients has been increasing, and it is estimated that the number of AD patients worldwide will reach 115 million by 2050. The average survival period of AD patients is only 5.5 years, and the disease has become the fourth leading cause of death in the elderly after he...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/48A61K33/04A61K33/24A61P25/28
Inventor 刘杰杨丽聪
Owner JINAN UNIVERSITY
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