Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A CQ-loaded nano-gold plugged mesoporous silica controlled release system and its preparation method and application

A technology of mesoporous silica and controlled release, which can be used in nervous system diseases, medical preparations of non-active ingredients, pharmaceutical formulas, etc. It can solve undiscovered problems, achieve premature release, inhibit Aβ polypeptide aggregation, Effect of reducing apoptosis-inducing ability

Inactive Publication Date: 2018-06-26
JINAN UNIVERSITY
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, no reports have been found on nano-gold plugging mesoporous silica controlled release system while inhibiting the aggregation of Aβ polypeptide induced by metal ions and the spontaneous aggregation of Aβ polypeptide

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A CQ-loaded nano-gold plugged mesoporous silica controlled release system and its preparation method and application
  • A CQ-loaded nano-gold plugged mesoporous silica controlled release system and its preparation method and application
  • A CQ-loaded nano-gold plugged mesoporous silica controlled release system and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Preparation of MSN-CQ-AuNPs

[0037]Preparation of MSN: Dissolve 0.1g of cetyltrimethylammonium bromide (CTAB) in 48mL of water and add 3.5mL of 0.2M NaOH. After the solution is heated to 85°C, stir vigorously for 1h; then, add 0.5mL of Tetraethyl silicate (TEOS), the mixed solution was stirred for 1 h; MSN was obtained by centrifugation, washed several times with ethanol and then centrifuged to remove ethanol; MSN was refluxed in HCl / methanol for 24 h to remove the surfactant CTAB; finally, centrifuged to obtain The MSNs were dried under vacuum at 60 °C overnight to remove the solvent in the mesoporous pores.

[0038] MSN-NH 2 Preparation: 1.0g MSN was dissolved in 80.0mL anhydrous toluene and sonicated for 5min. Subsequently, 1.0 mL of 3-aminopropyltriethoxysilane (APTES) was added and refluxed for 24 h to modify 3-aminopropyltriethoxysilane onto the surface of MSN to obtain MSN-NH2.

[0039] Preparation of MSN-BA: 400 mg of purified MSN-NH 2 Dissolve in...

Embodiment 2

[0043] Example 2 Inhibitory effect of MSN-CQ-AuNPs on the spontaneous aggregation of Aβ and the aggregation of Aβ polypeptide induced by metal ions

[0044] The degree of aggregation of Aβ40 polypeptide into fibers was detected by the dye ThT, and the fluorescence intensity of ThT was detected on a JASCO FP6500 fluorescence spectrophotometer. Aβ40 solution (30 μM) was incubated with 0.125, 0.25 and 0.5 mg / mL MSN or MSN-AuNPs at 37°C for 0 to 5 days. Aβ40 solution (30μM) with Cu 2+ (60 μM), 0.5 mg / mL MSN-CQ or MSN-CQ-AuNPs were incubated for 0 to 5 days. Take 50 μL from the incubation solution every day and incubate with 200 μL ThT (15 μM) for 15 min in the dark, and then detect the fluorescence intensity of ThT on a fluorescence spectrophotometer. ThT has an excitation wavelength of 440nm and an emission wavelength of 490nm. Experiments were repeated three times and standard deviations were calculated. Experimental results such as figure 2 As shown, MSN-CQ-AuNPs can inhi...

Embodiment 3

[0045] Example 3 Inhibitory effect of MSN-CQ-AuNPs on the spontaneous aggregation of Aβ and the neurotoxicity of Aβ polypeptide aggregation induced by metal ions

[0046] In this experiment, PC12 cells were purchased from ATCC Company, and the culture conditions were DMEM medium supplemented with 5% horse serum and 10% bovine serum, 5% CO 2 , at 37°C.

[0047] MTT test method: seed PC12 cells on a 96-well plate, 5×10 per well 3 Cells were cultured for 24 hours until the cells adhered to the wall. To detect the toxicity of Aβ40 fibrils, Aβ40 polypeptide (30 μM) was mixed with Cu 2+ (60 μM) and 0.5 mg / mL nanoparticles were incubated in cell culture medium at 37° C. for 3 days. Cells were then incubated in these incubation solutions for 72 h. To examine the toxicity of nanomaterials, the adherent PC12 (5×10 3 per well) were incubated with different concentrations of nanoparticles for 48 h. 60 μL / well of MTT solution was added 4 hours before the end of the culture. After 4 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to View More

Abstract

The present invention relates to the field of metal nanometer material preparation, and specifically discloses a CQ supporting nanometer gold blocking mesoporous silica controlled release system, a preparation method and applications thereof. The preparation method comprises: S1, carrying out amination modification on mesoporous silica MSN to obtain MSN-NH2, and carrying out 4-carboxy phenyl boronic acid modification to obtain MSN-BA; S2, dissolving the MSN-BA in PBS, carrying out ultrasonic dispersion, adding CQ, carrying out a stirring reaction for 12-24 h, and washing to obtain CQ-supporting MSN-BA; and S3, dissolving the CQ-supporting MSN-BA in PBS, adding monosaccharide-modified AuNPs, carrying out a stirring reaction for 12-24 h, and washing to obtain MSN-CQ-AuNPs. According to the present invention, the MSN-CQ-AuNPs can inhibit the spontaneous aggregation of Abeta, further has the inhibition effect on the metal ion induced Abeta polypeptide aggregation, and can be adopted as the potential Alzheimer's disease treatment drug.

Description

technical field [0001] The invention relates to the field of preparation of metal nanomaterials, in particular to a CQ-loaded nano-gold plugging mesoporous silica controlled release system and its preparation method and application. Background technique [0002] Alzheimer's disease (AD) is a progressive neurodegenerative disease characterized by memory and cognitive dysfunction. AD is more common in the elderly, and the prevalence of AD in people over 60 years old is about 5-10%, while the prevalence of AD in people over 85 years old is as high as 40-50%. With the aging of the population and the lack of effective means of prediction and treatment, the number of AD patients has been increasing. The average survival period of AD patients is only 5.5 years, and the disease has become the fourth leading cause of death in the elderly after heart disease, cancer and stroke. However, acetylcholinesterase inhibitors and N-methyl-D-aspartic acid (NMDA), which have entered into clin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/00A61K31/47A61K47/04A61K47/02A61P25/28
Inventor 刘杰杨丽聪周艳晖
Owner JINAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com