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Nanoparticles for Inhibiting Aβ and Metal Ion-Induced Aggregation and Its Preparation and Application

A nanoparticle and particle technology, applied in the field of nanomedicine, can solve the problems of non-selectivity, toxic and side effects of chelation, and achieve the effect of improving biocompatibility, simple method, and simple operation

Inactive Publication Date: 2017-08-11
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most metal chelating agents cannot penetrate the blood-brain barrier. In addition, metal chelating agents are not selective for the chelation of metal ions, which will chelate some necessary metal ions in the body and cause toxic side effects

Method used

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  • Nanoparticles for Inhibiting Aβ and Metal Ion-Induced Aggregation and Its Preparation and Application
  • Nanoparticles for Inhibiting Aβ and Metal Ion-Induced Aggregation and Its Preparation and Application
  • Nanoparticles for Inhibiting Aβ and Metal Ion-Induced Aggregation and Its Preparation and Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: the preparation of nano-selenium, nano-ruthenium and composite nano-selenium / ruthenium modified by arginine

[0039] (1) Weigh 0.1729g of sodium selenite, dilute to 10mL with distilled water to prepare a 0.1mol / L storage solution; at the same time weigh 0.0435g of arginine, dilute to 10mL with distilled water to prepare a 0.025mol / L storage solution ; Weigh 0.0259g of ruthenium trichloride, and make it to 5mL with distilled water to prepare a 0.025mol / L storage solution.

[0040] (2) Mix 0.2mL sodium selenite stock solution with different proportions of arginine, gradually add different proportions of sodium borohydride dropwise at 400rpm / min, stop stirring after the color changes to brick red, indicating that the The molar ratios of nano-selenium, sodium selenite to arginine or sodium borohydride are 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, respectively. Among them, the molar ratio of sodium selenite to arginine in arginine-modified nano-selenium was 1:4, which was...

Embodiment 2

[0044] Embodiment 2: the preparation of nano-selenium, nano-ruthenium and composite nano-selenium / ruthenium modified by gallic acid

[0045] (1) Weigh 0.1729g of sodium selenite, dilute to 10mL with distilled water to prepare a 0.1mol / L storage solution; at the same time weigh 0.0425g of gallic acid, dilute to 10mL with distilled water to prepare a 0.025mol / L storage solution; Weigh 0.0259 g of ruthenium trichloride, and dilute to 5 mL with distilled water to prepare a 0.025 mol / L storage solution.

[0046] (2) Take 0.2mL sodium selenite stock solution, gradually add different proportions of gallic acid dropwise under the condition of 400rpm / min, stop stirring after the color changes to brick red, indicating that nano-selenium has been reduced, and finally sodium selenite The molar ratios to gallic acid are 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, respectively. Among them, the molar ratio of sodium selenite to gallic acid in gallic acid-modified nano-selenium was the most stable. The a...

Embodiment 3

[0050] Example 3: Y-modified nano-selenium, nano-ruthenium and composite nano-selenium / ruthenium combined with Aβ1-40 polypeptide

[0051] First take 30μM Aβ 40 (purchased from Shanghai GL Biochem Ltd.) Incubated at 37°C for 3 days to form fibrous aggregates, and then added 60 μg / mL of Rhodamine B-labeled nanoparticles prepared in Example 1 and Example 2 to the sample Particles were incubated for another 6 h. Take 10 μL of the above solution and drop it on the glass slide whose surface has been treated with positive charge. After the sample is dried, observe it under a confocal laser microscope. The results show that the nano-selenium, nano-ruthenium and composite nano-selenium / ruthenium modified respectively by arginine, histidine, glucose, sucrose, vitamin C and gallic acid prepared by the present invention can be combined with Aβ 40 Polypeptide binding, wherein the amount of nano-ruthenium and composite nano-selenium / ruthenium binding to polypeptides is more than that of ...

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Abstract

The invention belongs to the technical field of nano-medicines, and discloses a nanoparticle for inhibiting Aβ and metal ion-induced Aβ aggregation, a preparation method and an application thereof. The nanometer particles are Y-modified nanometer ruthenium and / or selenium particles, and Y is at least one of arginine, histidine, glucose, sucrose, vitamin C and gallic acid. The Y-modified nanoparticles prepared by the present invention include nano-ruthenium, nano-selenium, and composite nano-selenium / ruthenium. The nanoparticles can not only inhibit the spontaneous aggregation of Aβ, but also have the effect of inhibiting the aggregation of Aβ polypeptide induced by metal ions, and can be applied to the preparation of Anti-Alzheimer's Syndrome Drugs. The vitamin C and gallic acid modified nanoparticles prepared by the present invention directly use vitamin C and gallic acid as the reducing agent and modifier, the preparation process does not need to add other auxiliary reagents, the product system is simple, the operation is simple, the method is simple, and the product can be directly stored and use.

Description

technical field [0001] The invention belongs to the technical field of nanomedicine, and in particular relates to a nano particle for inhibiting Aβ and metal ion-induced Aβ aggregation, a preparation method and application thereof. Background technique [0002] Alzheimer's disease (AD) is a progressive neurodegenerative disease characterized by memory and cognitive dysfunction, also known as senile dementia. AD is more common in the elderly, and the prevalence of AD in people over 60 years old is about 5-10%, while the prevalence of AD in people over 85 years old increases sharply, as high as 40-50%. With the aging of the population and the lack of effective means of prediction and treatment, the number of AD patients has been increasing, and it is estimated that the number of AD patients worldwide will reach 115 million by 2050. The average survival period of AD patients is only 5.5 years, and the disease has become the fourth leading cause of death in the elderly after he...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/54A61K33/04A61K33/24A61P25/28
Inventor 刘杰杨丽聪
Owner JINAN UNIVERSITY
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