Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation of traceable ultra-small nano-selenium and its application in Alzheimer's disease

An Alzheimer's disease, ultra-small technology, applied in the direction of nanotechnology, nanotechnology, in vivo test preparations, etc., can solve the problem that diagnostic technology cannot meet the needs of efficient and accurate detection, difficult to describe and measure, and no Al Alzheimer's disease and other problems, achieve the effect of inhibiting spontaneous aggregation, reducing amyloid plaques, and overcoming premature release

Active Publication Date: 2020-11-13
JINAN UNIVERSITY
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Whether nano-drugs can penetrate the blood-brain barrier and achieve therapeutic effects after entering the body, in the current medical diagnosis and treatment, the existing diagnostic technology cannot meet the needs of efficient and accurate detection, and the new and efficient imaging probe, Molecular imaging can be used to image the abnormal structure and function of the disease at the physiological and biochemical levels, which can provide more accurate information for the diagnosis and treatment of the disease
However, existing nanomedicines are difficult to describe and measure at the cell, molecular level, and in vivo, and detect their drug effects and blood-brain barrier permeability in real time.
Therefore, in order to evaluate the therapeutic effect in real time, it is of great significance to screen nano-drugs and provide a traceable nano-drug. However, there has been no report on the treatment of Alzheimer's disease with traceable ultra-small nano-selenium.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation of traceable ultra-small nano-selenium and its application in Alzheimer's disease
  • Preparation of traceable ultra-small nano-selenium and its application in Alzheimer's disease
  • Preparation of traceable ultra-small nano-selenium and its application in Alzheimer's disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1: Preparation of ultra-small nano-selenium modified by rhodamine

[0051] (1) Synthesis of ultra-small nano-selenium:

[0052] Add 10 mg of niobium selenide into 100 ml of distilled water, and keep stirring for 12 hours to obtain a uniformly dispersed solution. The resulting solution was added to a lined high-temperature reactor with a total volume of 150 ml, then the high-temperature reactor was sealed, and after a constant temperature reaction (at a temperature of 60° C.) for 4 hours, it was allowed to cool naturally to room temperature and allowed to stand for 6 hours. Then transfer the solution to a beaker and add 2mL of 40mM VC (vitamin C), stir for 10min under airtight conditions, adjust the pH to 8.0, and sonicate for 3h (pH 8.0) with a power of 250W. Afterwards, the solution was centrifuged for 15 minutes at a rotation speed of 10,000 rpm, and washed three times with absolute ethanol and water respectively. The resulting sample was dispersed into 3...

Embodiment 2

[0060] Example 2: Control Nano-Characterization

[0061] For comparison, normal nano-selenium (prepared in Example 1(3)①) was prepared to study the effect of particle size on the permeability of the blood-brain barrier. The size distribution and zeta potential of the nanoparticles were measured by a Malvern Zetasizer Nanzo ZS nanoparticle sizer. The results show that the particle diameters of nano-selenium and ultra-small nano-selenium (prepared in Example 1 (1)) in PBS buffer solution with pH=7.4 are 20nm and 4nm, respectively. see results figure 2 : The different potential and particle size changes indicate that according to different synthesis methods, we have successfully prepared two different sizes of nano-selenium.

Embodiment 3

[0062] Example 3: Ultra-small nano-selenium scavenging effect on free radicals in vitro

[0063] Detect the ultra-small nano-selenium (SeQDs) prepared in Example 1 (1) to the scavenging effect of excess free radicals, the specific process is as follows: SeQDs is added to H 2 o 2 Medium (SeQDs+H 2 o 2 ), the electron spin resonance (ESR) spectrum of the final reactant (i.e., DEMPO / COH adduct) was collected after 3 min of incubation, while the SeQDs alone and the H 2 o 2 is the control; among them, H 2 o 2 The final concentrations of SeQDs and SeQDs were 5 mM and 1 mg / mL, respectively. At the same time, adding SeQDs to H 2 o 2 After UV irradiation (SeQDs+H 2 o 2 +UV), with SeQDs plus UV irradiation (SeQDs+UV), H 2 o 2 UV irradiation (H 2 o 2 +UV), and SeQDs as controls. see results image 3 : Experimental results show that ultra-small nano-selenium can effectively eliminate H 2 o 2 Oxidation produces strength.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses preparation of trackable ultra-small nano-selenium and an application thereof in AD (Alzheimer's disease). The preparation method of the trackable ultra-small nano-selenium comprises the following steps: adding niobium selenide to water, performing constant-temperature treatment under a closed condition after uniform stirring, cooling the solution to room temperature aftertreatment, and leaving the solution to stand to obtain a mixed solution; adding VC to the mixed solution, performing stirring under the sealed condition and performing ultrasonic treatment, and performing centrifugation and washing to obtain the trackable ultra-small nano-selenium. The prepared trackable ultra-small nano-selenium can be used as a nanoprobe and penetrates through blood brain barrier to arrive at infected parts of AD under the lipid raft mediated endocytosis, the defects of early release of complex nano-drugs and penetration difficulty in blood brain barrier are effectively overcome, the purpose of rapidly treating the AD is achieved, and the treatment effect of the AD can be detected in real time through fluorescence imaging.

Description

technical field [0001] The invention belongs to the technical field of nano-medicines, in particular to the preparation of traceable ultra-small nano-selenium and its application in Alzheimer's disease. Background technique [0002] Alzheimer's disease (AD) is the most common complex central neurodegenerative disease, and its key pathological markers are brain extracellular amyloid plaques, intracellular neurofibrillary tangles (NFT) and extensive neuronal damage. The pathogenesis of AD is still unclear, but in the latest reported studies, it was shown that due to the excessive production and aggregation of Aβ, a large amount of reactive oxygen species such as H 2 o 2 . Increased reactive oxygen species can cause inflammatory responses, induce hyperphosphorylation of Tau protein, and lead to nerve cell dysfunction, etc. These pathological processes interact and eventually form a vicious circle, which promotes the occurrence and progression of AD. For the pathological tre...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C01B19/02B82Y40/00B82Y30/00A61K33/04A61K49/00A61P25/28
Inventor 刘杰孙婧刘亚楠周慧龚有聪
Owner JINAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com