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A method for quantifying polysaccharides and their components

A polysaccharide and component technology, applied in the field of sugar determination, can solve the problems of poor accuracy and cumbersome operation, and achieve the effect of simple method and high accuracy of results

Active Publication Date: 2017-01-25
李绍平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention can overcome the deficiencies of poor accuracy and cumbersome operation in the prior art

Method used

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  • A method for quantifying polysaccharides and their components
  • A method for quantifying polysaccharides and their components
  • A method for quantifying polysaccharides and their components

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Prepare standard polysaccharide solution: Use 0.9% NaCl solution to prepare series of concentrations (0.2, 0.4, 0.6, 0.8, 1.0, 1.2 mg / mL) dextran 25, dextran 270, dextran 410, arabinogalactan, arabinan, dextran Mannan, oat glucan, and xyloglucan solutions, after passing through a 0.45 μm microfiltration membrane, measure the dn / dc of each standard polysaccharide with a differential refractive index detector; use 0.9% NaCl solution to prepare dextran 25, dextran 270, dextran 410, Arabinogalactan, arabinan, glucomannan, oat glucan, and xyloglucan to a final concentration of 1 mg / mL, 2 mg / mL, and 4 mg / mL respectively, passed through a 0.45 μm microfiltration membrane for Determination of standard polysaccharide content;

[0027] Determination of the incremental value of the specific refractive index (dn / dc): use a differential refractive index detector (RI) to measure the RI signal at the concentration of each standard polysaccharide series, and calculate the dn / dc value o...

Embodiment 2

[0036] Extraction of polysaccharides from different samples to be tested: take Cordyceps sinensis, Cordyceps militaris, red lucidum, purple lucidum, pine and fir ganoderma, ginseng, Panax notoginseng, bamboo ginseng, palm ginseng, astragalus, bamboo fungus, shiitake mushrooms, Poria cocos, Hericium erinaceus, tremella, fungus, Green tea, oolong tea, cassia seed, dendrobium, medlar, longan, etc. 1g, several parts, add 20 times the weight-volume ratio of water, use microwave-assisted water extraction, the extraction conditions are power 600W, time 15min; the extract is centrifuged to obtain the supernatant solution, concentrated under reduced pressure to 10mL, added 4 times the volume of 95% ethanol, left to settle overnight at 4°C, washed the precipitate with 95% ethanol after centrifugation, then redissolved the precipitate with 10mL hot water (60°C), and centrifuged to take the supernatant solution to obtain crude polysaccharide extract. The crude polysaccharide is freeze-dri...

Embodiment 3

[0048] Extraction of polysaccharides from samples from three sources: take 1 g each of Panax notoginseng, ginseng, and bamboo fungus, and several parts, and the rest of the operations are the same as the sample polysaccharide extraction steps in Example 2.

[0049] Preparation of sample polysaccharide solution: same as in Example 1.

[0050] On-line separation and multi-angle laser light scattering (MALLS), differential refractive index (RI) and ultraviolet (UV) detection combined technical analysis: the chromatographic column is TSK-GEL G-6000PW XL (300mm×7.8mm, i.d.) Series TSK-GEL G-3000PW XL (300mm * 7.8mm, i.d.), all the other steps are with the "on-line separation and multi-angle laser light scattering (MALLS), differential refraction (RI) and ultraviolet (UV) detection combined technical analysis" step of embodiment 1.

[0051] The polysaccharide average dn / dc value is used to calculate the polysaccharide content of the sample: the polysaccharide average dn / dc value (0...

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Abstract

The invention relates to the technical field of saccharide measurement and in particular relates to a method for quantifying polysaccharides and components of the polysaccharides. The method comprises the following steps: performing high performance size exclusion chromatography (HPSEC) or asymmetric field flow separation on a polysaccharide sample solution, analyzing by combining technologies of laser light scattering (LLS), refractive index (RI) and ultraviolet detection (UV), and calculating the contents of polysaccharides and components of the polysaccharides in a selected molecular weight distribution range through the dn / dc values of the polysaccharides and components of the polysaccharides measured by adopting the refractive index increment value (dn / dc) of 0.146mL / g (0.130-0.160mL / g) or a refractive index detection technology. The method can be used for measuring the content of the polysaccharides in plants and fungi and products of the polysaccharides, and the accurate, high-efficiency and simple method is provided for quality control on polysaccharide substances.

Description

technical field [0001] The invention relates to the technical field of sugar determination, in particular to a method for quantifying polysaccharides and their components. Background technique [0002] Polysaccharides are a class of highly dispersed, polysaccharide carbohydrates formed by the condensation of monosaccharide molecules. As an essential component of living organisms, it is closely related to life-sustaining energy metabolism and various physiological functions. Modern pharmacological research has proved that carbohydrates have a wide range of biological activities and pharmacological effects, and have been called the four basic substances of life together with proteins, nucleic acids, and lipids. In recent years, polysaccharides derived from fungi, plants, and marine products have attracted people's attention due to their significant and specific biological activities, such as anti-cancer, anti-tumor, immune regulation, lowering blood sugar and blood lipids, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 李绍平赵静吴定涛张杰良
Owner 李绍平
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