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Mycoplasma hyorhinosus strain and its inactivated vaccine and application

A technology of Mycoplasma hyorrhea and strains, applied in the directions of bacteria, antibacterial drugs, bacterial antigen components, etc., can solve the problems of increased mortality of weaned piglets, economic losses of pig farming, etc., and achieve good immunogenicity and stable titer. Effect

Active Publication Date: 2016-08-17
泰州博莱得利生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Clinically, Mhr is often co-infected with porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2), causing severe porcine respiratory syndrome and significantly increasing the mortality rate of weaned piglets. huge economic losses

Method used

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  • Mycoplasma hyorhinosus strain and its inactivated vaccine and application
  • Mycoplasma hyorhinosus strain and its inactivated vaccine and application
  • Mycoplasma hyorhinosus strain and its inactivated vaccine and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Isolation and culture identification of Mycoplasma hyorhinois WH-C strain

[0030] 1 Sources and standards of strains

[0031] According to the declaration requirements of new biological products, combined with a large amount of test data obtained by the present invention, and referring to "Chinese Veterinary Pharmacopoeia" (2005 edition), the strains for seedling production were identified.

[0032] 1.1 Source of bacteria

[0033] The bacterial species used in the present invention is Mycoplasma hyorhinosum WH-C strain, which is obtained by the inventor after isolation and domestication from diseased pigs. Since this bacterial strain is produced from pregnant sows that have typical PMWS and suffer from respiratory syndrome Compared with other isolated strains, it has a higher proliferation titer on the medium and has better immunogenicity to pigs, so it is selected for vaccine production. The culture medium for Mycoplasma hyorhinosum (horse serum (HPS) is a...

Embodiment 2

[0055] The preparation of embodiment 2 inactivated vaccine

[0056] The preparation process of the Mycoplasma hyorhini WH-C strain is prepared according to the current mature commercial vaccine preparation process. Judging from the quality inspection results of 3 batches of vaccines prepared in the inventor's laboratory and 5 batches of vaccines produced in the middle, the quality of the vaccine products prepared by this process flow is stable, and the efficacy inspection results fully meet the established quality standards.

[0057] 1 Preparation of bacteria solution

[0058] During the reproduction process, many factors will affect the proliferation of Mycoplasma hyorhinois WH-C strain. After a series of comparative experiments, the components of the culture medium for Mycoplasma hyorhinosum were selected as follows: horse serum (HPS) was a product of PAA Company, PPLO broth was a product of BD Company, and yeast extract was a product of British OXOID Company; of bacteria ...

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PUM

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Abstract

The invention discloses a mycoplasma hyorhinis strain and an inactivated vaccine and application thereof. The mycoplasma hyorhinis strain is screened and cultivated by use of a mycoplasma hyorhinis virulent strain separated from a pig farm, is good in immunogenicity and stable in titer, and has an accession number of CGMCC (China General Microbiological Culture Collection Center) NO.8701; and during the preparation of the inactivated vaccine by use of the virulent strain, the variety of inactivating agents and adjuvants and other process conditions can be optimized. The production technology, safety, protection rate, immunization programs, the minimum dose, antibody duration period, storage period and the like of the inactivated vaccine can be detected by experiments, and the experiments finally prove that the prepared inactivated vaccine is safe and effective, can effectively prevent and cure porcine polyserositis, pneumonia, pericarditis, pleurisy, arthritis or otitis media and other diseases caused by mycoplasma hyorhinis.

Description

technical field [0001] The present invention relates to a strain of Mycoplasma bacterial strain, relate in particular to a virulent bacterial strain of Mycoplasma hyorhina isolated, the present invention further relates to the inactivated vaccine prepared by this virulent bacterial strain of Mycoplasma hyorhina, the present invention further relates to their in the prevention or treatment caused by Mycoplasma hyorhina. The invention relates to the application in various diseases of pigs, and belongs to the field of isolation and application of mycoplasma hyorhina strains. Background technique [0002] Mycoplasma hyorhinis (Mhr) is the main pathogen that causes polyserositis, pneumonia, pericarditis, pleurisy, arthritis, otitis media and other diseases in pigs. The disease has a high incidence and low mortality in pigs. Clinically, Mhr is often co-infected with porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2), causing severe por...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61K39/02A61P11/00A61P9/00A61P19/02A61P27/16A61P31/04C12R1/35
Inventor 刘继红
Owner 泰州博莱得利生物科技有限公司
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