Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

A method for improving the seedling rate of pitaya tissue cultured seedlings

A technology of tissue culture seedlings and seedling rate, which is applied in the field of plant propagation, can solve the problems of low seedling rate of pitaya tissue culture seedlings, and achieve the effects of high induction rate of adventitious buds, short production cycle and vigorous growth

Inactive Publication Date: 2016-04-13
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is to overcome the low defect and deficiency of existing pitaya tissue culture seedling seedling rate, to improve the seedling rate of pitaya tissue culture seedling, reduce the production cost of seedling more effectively, provide a A kind of method that can improve the seedling rate of dragon fruit tissue culture seedling

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for improving the seedling rate of pitaya tissue cultured seedlings
  • A method for improving the seedling rate of pitaya tissue cultured seedlings
  • A method for improving the seedling rate of pitaya tissue cultured seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1 The present invention improves a method for the seedling rate of pitaya tissue culture seedlings, and its steps are as follows:

[0017] (1) Adventitious bud induction

[0018] ① Selection and treatment of explants: select semi-lignified dragon fruit stems that are free from diseases and insect pests, wash them with washing powder water first, and rinse them with tap water, then cut the stems longitudinally, remove the pith, and then cut into Stem slices with a width of 2cm to 3cm and a length of 3cm to 4cm. The outer skin of each stem slice has a complete thorn seat, and the fluff and small thorns on the thorn seat are retained.

[0019] ② Adventitious bud induction: Disinfect the treated stem slices in 75% alcohol for 30 seconds, wash once in sterile water, transfer to 2% sodium hypochlorite solution for disinfection for 10 minutes, and then sterilize them in sterile water. Wash 3 to 5 times, after the disinfection is completed, cut off the surface of th...

Embodiment 2

[0026] Embodiment 2 is the same as Embodiment 1 except that the following measures are different, and will not be repeated here.

[0027] The adventitious bud induction medium of step (1) ② is: MS+TDZ0.4mg / L+NAA0.1mg / L+sucrose 3%+agar 0.6%, pH value is 5.8; The seedling growth medium of step (2) is: MS+6-BA4mg / L+NAA0.1mg / L+activated carbon 1g / L+sucrose 3%+agar 0.6%, pH value is 5.8.

[0028] The test effect of above each embodiment:

[0029] Pitaya tissue culture seedlings become seedlings: refer to the main stem with basal roots and / or the side shoots with aerial roots of the adventitious buds that are inserted into the above-mentioned seedling medium to proliferate (promptly grow out).

[0030] These main stems with basal roots and side branches with aerial roots have roots, stems, and leaves (the small thorns of dragon fruit are its leaves, which are metamorphic leaves), and they become complete regenerated plants after being cut off respectively. .

[0031] The seedling...

Embodiment 1～2

[0041] Table 1 embodiment 1~2 pitaya tissue culture seedlings seedling-forming effect

[0042]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for improving the seedling rate of tissue culture seedlings of hylocereus undulatus britt. The method comprises the following steps: carrying out adventitious bud induction, synchronous multiplication and rooting seedling culture, wherein hylocereus undulatus britt barbed seats and lignification shoots with small barbs and fluff on the barbed seats are used as explants; when lateral branches of adventitious buds grow, base roots of adventitious bud base parts grow and aerial roots of the lateral branches grow, carrying out hardening seedling; after the hardening seedling, taking out seedlings from a bottle; cutting off the lateral branches with the aerial roots and main stems with the base roots and planting in a culture medium; after the lateral branches and the main stems are survived, growing new roots; and putting the lateral branches and the main stems into pots. According to the method, the production period of the tissue culture seedlings of the hylocereus undulatus britt is short, the adventitious bud induction rate is high, the multiplication and the rooting are synchronous, the multiplication coefficient is more than 5.45 and the rooting rate is 100%; the growth vigor of multiplied seedlings is strong and the transplanting survival rate is more than 95%; one inoculated adventitious bud can grow 3-4 lateral branches with the aerial roots, namely one inoculated adventitious bud can grow 4-5 grown-up seedlings with the roots; the seedling rate of the tissue culture seedlings of the hylocereus undulatus britt is increased by 3-4 times.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and in particular relates to a method for improving the seedling rate of dragon fruit tissue culture seedlings. Background technique [0002] Pitaya scientific name (Hylocereus undatus (Haw.) Britt. & Rose.), also known as red dragon fruit, green dragon fruit, fairy honey fruit, etc., is a plant of the genus Hylocereus (Cactaceae) in the family Cactaceae. The fruit of dragon fruit is not only beautiful in appearance, but also has a health care effect. It has the effects of clearing blood, reducing fire and lowering cholesterol. Regular consumption can promote human metabolism and improve immune function. In addition, its cultivation is relatively easy, with few pests and diseases, early fruit and high yield, and can adapt to drought Climate is a new type of fruit with great potential for market development. In recent years, Yunnan, Guizhou, Guangxi, Guangdong and other provinces are vi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 彭绿春瞿素萍张艺萍苏艳王丽花杨秀梅张丽芳
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com