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New application of mucor racemosus

A technology of Mucor racemosa and Mucor fungus, applied in the field of microorganisms, can solve problems such as Mucor racemosa that have not yet been seen, and achieve the effects of high enzymatic hydrolysis efficiency and broad application prospects

Active Publication Date: 2014-07-16
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Racemosa is a eukaryotic microorganism that can secrete protease. It has been found that Mucor racemosa can be used to prepare fermented bean curd and has the function of transforming steroid drug intermediates. There is no evidence of Mucor racemosa in soybeans in domestic and foreign literature. Related reports on the conversion of isoflavones

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] This example illustrates the acquisition of Mucoris racemosa.

[0028] Inject Mucor racemosa into the slant culture medium under sterile conditions, and culture at 30°C for 72h. Seed culture: Inject the slant bacteria into the seed medium under sterile conditions, 30°C, shaker 160 r. min -1 , cultivated for 48h. Fermentation culture: put the liquid seed culture medium into the fermentation medium, shake the bed at 160 r. min -1 , and cultivated at 30°C for 72h.

[0029] Among them, slant medium: 5 g of potato dipping powder, 20 g of glucose, 20 g of agar, 1000 ml of distilled water, and sterilized at 121° C. for 20 min. Seed medium: 2% soluble starch, 1% glucose, 0.1% potassium dihydrogen phosphate, 0.1% magnesium sulfate, 0.5% yeast extract, 0.2% sodium nitrate, sterilized at 121°C for 20 minutes. Enzyme-producing fermentation medium: 4% defatted soybean meal, 2% bran, 0.1% peptone, 0.1% ammonium sulfate, 0.1% potassium dihydrogen phosphate, 0.1% s...

Embodiment 2

[0032] It is 5mg. mL -1 , then weighed 100 mg of soybean isoflavone powder, added 10 ml of Mucor racemosa containing β-glucosidase, i.e. 50 mg of Mucor racemosa, and hydrolyzed it for 2 h at pH 5.5 and hydrolysis temperature of 50 ° C. The above hydrolyzate was taken out and boiled to inactivate the enzyme, filtered through filter paper, extracted twice with ethyl acetate, evaporated with a rotary evaporator, added 5ml of chromatographic methanol, then mixed, and put into a 10ml volumetric flask to constant volume. Take an appropriate amount to filter through a filter, and test on the machine. The chromatographic column is a kromasil C18column column (4.6mm×150mm, 5um); the mobile phase: methanol-water (30:70), and the flow rate is 1.00ml. min -1 , the detection wavelength is 260nm, and the column temperature is 25°C. Injection volume: 10μl, retention time 10min, determination of soybean isoflavone aglycone, the concentration of aglycone is 18.32mg. mL -1 .

Embodiment 3

[0034] It is 5mg. mL -1 , then weighed 100 mg of soybean isoflavone powder, added 30 ml of Mucor racemosa containing β-glucosidase, i.e. 150 mg of Mucor racemosa, and hydrolyzed for 2.5 h at pH 6.5 and hydrolysis temperature of 60° C. The above hydrolyzate was taken out and boiled to inactivate the enzyme, filtered through filter paper, extracted twice with ethyl acetate, evaporated with a rotary evaporator, added 5ml of chromatographic methanol, then mixed, and put into a 10ml volumetric flask to constant volume. Take an appropriate amount to filter through a filter, and test on the machine. The chromatographic column is a kromasil C18column column (4.6mm×150mm, 5um); the mobile phase: methanol-water (30:70), and the flow rate is 1.00ml. min -1 , the detection wavelength is 260nm, and the column temperature is 25°C. Injection volume: 10μl, retention time 10min, determination of soybean isoflavone aglycone, the concentration of aglycone is 21.51mg. mL -1 .

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PUM

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Abstract

The invention relates to an application of mucor racemosus in production of free aglycones by hydrolyzing soy isoflavones. The invention also provides a method for producing free aglycones by hydrolyzing soy isoflavones by using the mucor racemosus. The invention provides a new use of the mucor racemosus in production of the free aglycones by hydrolyzing the soy isoflavones, and the method is high in enzymolysis efficiency and can be applied to preparing the free aglycones of the soy isoflavones; a new thought is developed for the preparation of the free aglycones, and the new use of the mucor racemosus has wide application prospect.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a new application of Mucor racemosus, in particular to the application of Mucor racemosus in hydrolyzing soybean isoflavones to produce free aglycon. Background technique [0002] Soybean isoflavones have many physiological functions such as preventing cancer, preventing osteoporosis, improving women's menopausal syndrome, preventing cardiovascular diseases, etc. It has become a hot research topic of scientists from all over the world in recent years. Nearly 99% of soy isoflavones in soybeans exist in the form of β-glucoside, and only about 1% is free aglycone. The glycoside type has low activity and is difficult to be directly absorbed by the human body. It must be hydrolyzed by human microorganisms to form an aglycon form before it can be easily absorbed. In vitro experiments also show that soybean isoflavone aglycone has better estrogen-like biological activity than soybean isofl...

Claims

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Application Information

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IPC IPC(8): C12P17/06C12R1/785
Inventor 李丽阳吴志军葛文中阮洪生
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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