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Method for biologically synthesizing poly-3-hydroxypropionic acid

A technology for synthesizing enzymes and malonyl coenzymes, which is applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of inability to apply industrialized production, complex fermentation processes, and high production costs, and achieve low cost. , the effect of wide source of raw materials and low cost

Inactive Publication Date: 2014-07-02
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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Problems solved by technology

However, the biosynthesis of P3HP currently requires the addition of related carbon sources such as 3-hydroxypropionic acid, 1,5-pentanediol, and 1,7-heptanediol. These related carbon sources are expensive, which makes the production cost of P3HP too high , cannot be applied to industrial production
The research on the synthesis of P3HP by biological methods using unrelated carbon sources has just started. There is only one literature report that Andreessen et al. used engineering Escherichia coli to produce P3HP with glycerol as a carbon source (Andreessen et al. complex

Method used

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  • Method for biologically synthesizing poly-3-hydroxypropionic acid
  • Method for biologically synthesizing poly-3-hydroxypropionic acid
  • Method for biologically synthesizing poly-3-hydroxypropionic acid

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Embodiment 1

[0097] By co-overexpressing the acetyl-CoA carboxylase gene (acc) and propionyl-CoA synthetase gene (prpE) from Escherichia coli (E.coli), derived from C.aurantiacus (orange green flexure The malonyl-CoA reductase gene (mcr) from R.eutropha (Alcaligenes eutropha) and the polyhydroxy fatty acid synthase gene (phaC) from R. Poly-3-hydroxypropionic acid.

[0098] Those skilled in the art should understand that the co-cloning of the above four genes into Escherichia coli cells is carried out in accordance with standard molecular cloning techniques. Moreover, according to actual needs, appropriate E. coli codon optimization can be performed on genes not derived from E. coli, so as to be better expressed in E. coli. In the present invention, the inventors found that the malonyl-CoA reductase gene (mcr) derived from C. aurantiacus (Chloroflexus aurantiacus) can be expressed in E. For good expression, the polyhydroxy fatty acid synthase gene (phaC) from R. eutropha (Alcaligenes eutr...

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Abstract

The invention provides a recombinant escherichia coli strain, a preparation method of the recombinant escherichia coli strain and a method for biologically synthesizing poly-3-hydroxypropionic acid from acetyl coenzyme A. The method comprises the following steps: with glucose or glycerin and the like as carbon sources, commonly over-expressing endogenous or exogenous acetyl coenzyme A carboxylase genes (acc) and propionyl coenzyme A synthetase genes (prpE) as well as exogenous malony coenzyme A reductase genes (mcr) and polyhydroxyalkanoate synthetase genes (phaC) in proper host cells (such as escherichia coli), and degrading intermediate products by virtue of glucose so as to biologically synthesize the poly-3-hydroxypropionic acid, wherein acetyl coenzyme A is finally obtained from simple starting materials such as the glucose and the like.

Description

technical field [0001] The present invention relates to the biosynthesis of poly-3-hydroxypropionic acid. Specifically, the present invention provides a recombinant Escherichia coli engineering strain, a preparation method of the strain and a method for biosynthesizing poly-3-hydroxypropionic acid from acetyl-CoA using the recombinant Escherichia coli engineering strain. Background technique [0002] Poly(3-hydroxypropionate), P3HP is a very promising biodegradable plastic with excellent biomaterial properties and mechanical properties, such as high mechanical strength and tensile strength (> 400MPa), high elongation at break (>300%), biodegradability, biocompatibility, insoluble in water, non-toxic, piezoelectric, thermoplastic, etc. Compared with the other two biodegradable plastics, poly 3-hydroxybutyric acid (PHB) and polylactic acid (PLA), which have been studied more, P3HP has superior performance: it has better stability than PLA and will not be hydrolyzed , a...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12P7/62C08L67/04C12R1/19
Inventor 咸漠赵广王琦刘长水
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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