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A screening method for drought-resistant germplasm

A screening method and drought resistance technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of sample death, low accuracy, difficult control, etc., and achieve high screening accuracy and repeatability. , the effect of uniform medium

Inactive Publication Date: 2016-01-13
GUIZHOU TOBACCO SCI RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The germination beds of seeds mainly include paper beds, sand beds and soil beds. Paper beds are mainly used for tobacco seeds, because tobacco seeds are very small, and the particles of sand beds and soil beds are relatively large. If sand beds or soil beds are used Tobacco seeds are easy to lose, but the paper bed has disadvantages such as cumbersome operation, difficult control, and low accuracy. If no water is poured during the test, it will easily cause excessive drought, resulting in the death of all samples, and it is difficult to distinguish drought resistance. Sprinkling water can not control the amount, too little effect is not obvious, and too much may not achieve the purpose of the drought test
In addition, whether it is a paper bed, a sand bed or a soil bed, there are defects such as uneven moisture distribution, poor repeatability, and low screening accuracy.

Method used

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  • A screening method for drought-resistant germplasm

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Embodiment 1

[0025] 1. Preparation of the hole punch: On a square wooden board with a side length of 70mm, fix 10 rows of sticks with a diameter of 1mm and a length of 1cm, 10 sticks per row, two adjacent rows and two adjacent columns The distance is 4mm.

[0026] 2. Insert the above-mentioned hole punch stick down into the Petri dish.

[0027] 3. Use an SX-500 autoclave to dissolve the agar powder to obtain the agar solution of the required concentration. Pour the obtained agar solution into the petri dish in step 2, so that the thickness of the liquid agar is 6 mm. After cooling, pull it out vertically and gently The hole punch was used to leave 100 seeding holes on the agar bed.

[0028] Prepare agar beds with a concentration of 17% and 22% according to the above method, and then plant Nanjiang No. 3, Honghua Dajinyuan and Yunyan 87 in the holes on the above agar bed.

[0029] Spread 2 pieces of filter paper in a Petri dish, wet the filter paper with distilled water (1-1.5ml / 100cm 2 ...

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Abstract

The invention discloses a drought-resistance seed sieving method. An agar bed is adopted for sieving. When the method is adopted, the seed sprouting media are very uniform, in addition, the repeated differences are very small and can be ignored, drought-resistance materials can be sieved out from a great number of varieties reaching thousands of parts, the efficiency is high, the repeatability is good, the accuracy is high, the operation is simple, the water dispersion is uniform, and the water supplementation operation is not needed in the sieving process.

Description

technical field [0001] The invention relates to a method for screening drought-resistant germplasm. Background technique [0002] Drought is one of the main environmental factors affecting plant growth and development. Tobacco often encounters drought during the growth process, especially from the cluster stage to the flourishing stage. Drought has become an important factor affecting vegetative growth. The screening of drought-resistant seeds has become one of the effective ways to solve the above problems. [0003] Traditionally, the method of identifying drought resistance is mainly field identification, but the field identification period is long, the repeatability is poor, and it is greatly affected by the cultivation environment. Therefore, it is more convenient and fast to choose identification from seed germination to seedling growth stage. The germination beds of seeds mainly include paper beds, sand beds and soil beds. Paper beds are mainly used for tobacco seeds...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00A01G7/00
Inventor 李振华叶定勇陈尧刘一灵管昌杰杨贵生任学良赵杰宏
Owner GUIZHOU TOBACCO SCI RES INST
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