A screening method for drought-resistant germplasm
A screening method and drought resistance technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of sample death, low accuracy, difficult control, etc., and achieve high screening accuracy and repeatability. , the effect of uniform medium
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[0025] 1. Preparation of the hole punch: On a square wooden board with a side length of 70mm, fix 10 rows of sticks with a diameter of 1mm and a length of 1cm, 10 sticks per row, two adjacent rows and two adjacent columns The distance is 4mm.
[0026] 2. Insert the above-mentioned hole punch stick down into the Petri dish.
[0027] 3. Use an SX-500 autoclave to dissolve the agar powder to obtain the agar solution of the required concentration. Pour the obtained agar solution into the petri dish in step 2, so that the thickness of the liquid agar is 6 mm. After cooling, pull it out vertically and gently The hole punch was used to leave 100 seeding holes on the agar bed.
[0028] Prepare agar beds with a concentration of 17% and 22% according to the above method, and then plant Nanjiang No. 3, Honghua Dajinyuan and Yunyan 87 in the holes on the above agar bed.
[0029] Spread 2 pieces of filter paper in a Petri dish, wet the filter paper with distilled water (1-1.5ml / 100cm 2 ...
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