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Liquid-phase chip kit for detecting inflammatory markers in gingival crevicular fluid

A liquid phase chip and marker technology, applied in the field of medicine and biology, can solve the problems of poor guidance of patient treatment, low sensitivity, low detection rate, etc., and achieve simple and reliable preparation method, good stability and repeatability Good results

Inactive Publication Date: 2014-05-28
BEIJING STOMATOLOGY HOSPITAL CAPITAL MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods are single-index detection
However, the single-index detection of the secretion components of gingival crevicular fluid reflects the state of the periodontal microenvironment, which has the disadvantages of low specificity and low sensitivity, especially the detection rate of early inflammation and bone resorption is not high, so it cannot be very accurate. Good guide to patient treatment
In addition, the amount of gingival crevicular fluid sample required for single-index detection is relatively large, and the cost is expensive.

Method used

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  • Liquid-phase chip kit for detecting inflammatory markers in gingival crevicular fluid
  • Liquid-phase chip kit for detecting inflammatory markers in gingival crevicular fluid
  • Liquid-phase chip kit for detecting inflammatory markers in gingival crevicular fluid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Preparation of a liquid chip kit for detection of inflammatory markers in gingival crevicular fluid

[0047] 1 kit composition

[0048] (1) 3-plex coated microspheres: Contains different color-coded microspheres coated with IL-1β capture antibody, IL-6 capture antibody and Osteocalcin capture antibody;

[0049] (2) 3-plex biotin-labeled detection antibodies: respectively use biotin-labeled IL-1β, IL-6 and Osteocalcin detection antibodies;

[0050] (3) streptavidin phycoerythrin.

[0051] Wherein, the capture antibodies are: IL-1β capture antibody, clone number is P2H3; IL-6 capture antibody, clone number is B-E8; Osteocalcin capture antibody, clone number is 1-22.

[0052] The detection antibodies are: IL-1β detection antibody, the clone number is EP408Y; IL-6 detection antibody, the clone number is E457; Osteocalcin detection antibody, the clone number is aa59-74.

[0053] 2 Preparation method of kit

[0054] Include the following steps:

[0055] (1) The...

Embodiment 2

[0073] Example 2 Application of liquid chip kit for detection of inflammatory markers in gingival crevicular fluid

[0074] 1 Experiment purpose

[0075] Combined detection of IL-1β, IL-6 and Osteocalcin three markers in gingival crevicular fluid of patients with fixed denture repair at different times.

[0076] 2 Subjects

[0077] 1) On the premise of informed consent and meeting the inclusion conditions, select the enrolled cases and record personal basic information. 2) Record the basic information of the patient's fixed denture restoration: including the position of the missing tooth before treatment, the number of abutment teeth, the periodontal condition of the abutment teeth, the looseness of the abutment teeth, the occlusal condition, and the condition of the abutment teeth with the same name. 3) Determine the changes in GCF secretion and the factors contained in the abutment teeth before restoration (control group) and after restoration; use the filter paper strip m...

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Abstract

The invention provides a liquid-phase chip kit for detecting inflammatory markers in gingival crevicular fluid. The liquid-phase chip kit comprises (1) a 3-plex-wrapped microsphere, (2) a 3-plex biotin marking detection antibody, and (3) streptavidin phycoerythrin, wherein the 3-plex-wrapped microsphere comprises encoded microspheres which are respectively coated with IL-1beta capturing antibodies, IL-6 capturing antibodies and Osteocalcin capturing antibodies and have different colors, and the 3-plex biotin marking detection antibody comprises an IL-1beta detection antibody, an IL-6 detection antibody and an Osteocalcin detection antibody which are marked by biotin. The invention also provides a preparation method for the kit. The liquid-phase chip kit for detecting the inflammatory markers in the gingival crevicular fluid has the advantages of high flux, parallel multi-index detection, quickness and accuracy in detection, low cost and the like. Experiments show that the forecast accuracy of the incidence rate of adverse reactions and inflammatory risks of tissues around teeth can reach more than 90 percent after a fixed partial denture is repaired through the adoption of the kit provided by the invention.

Description

technical field [0001] The invention belongs to the category of medical biology, and in particular relates to a liquid phase chip kit and a method thereof for detecting inflammatory markers in gingival crevicular fluid. Background technique [0002] Dental integrity is an important manifestation of human health and longevity and quality of life. For patients with missing teeth, fixed dentures are used to repair the missing teeth, and the abutment teeth bear the masticatory force of the missing teeth to repair the dentition defect. Fixed dentures have light foreign body sensation when worn in the mouth, and have the characteristics of comfort, appearance, and good chewing function, but the price of several thousand to tens of thousands of yuan is higher than the cost of partial denture repair. At the same time, the fixed denture is glued in the patient's mouth. The function of the denture is closely related to the health of the abutment teeth. However, patients often do not ...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/531
CPCG01N33/54313G01N33/531G01N2800/18G01N2800/7095
Inventor 李茵张振庭
Owner BEIJING STOMATOLOGY HOSPITAL CAPITAL MEDICAL UNIV
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