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Saccharomyces cerevisiae for producing ribonucleic acid by fermentation, and application thereof

A technology of Saccharomyces cerevisiae and ribonucleic acid, applied in the direction of microorganism-based methods, fermentation, microorganisms, etc., can solve the problems of low yield and achieve the effects of genetic stability, high mutation rate, and stable high-yield traits

Active Publication Date: 2014-05-28
NANJING BIOTOGETHER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The RNA content in brewer's yeast can generally reach 6% to 8%. Up to now, RNA production from waste brewer's yeast has been widely used to extract RNA, which has the advantages of simple process and low cost, but the yield is low and cannot meet the needs of the market.

Method used

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  • Saccharomyces cerevisiae for producing ribonucleic acid by fermentation, and application thereof
  • Saccharomyces cerevisiae for producing ribonucleic acid by fermentation, and application thereof
  • Saccharomyces cerevisiae for producing ribonucleic acid by fermentation, and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Taking the ribonucleic acid-producing strain isolated from the laboratory as the starting strain, the N + Ion beam mutagenesis (the vacuum degree in the target chamber of the low-energy ion implanter is 2×10 －4 , the injection energy is 50kev, and the dose is 5×10 13 ions cm -2 ·s -1 ) after the spore suspension was diluted 100 times with sterile water, 0.1ml was spread on the wort plate medium and cultured at 30°C for 3 days, from which a single colony was selected and cultured on the wort slant for 3 days. Transfer one loop to 500mL of 30mL liquid seed medium (molasses 30g / L, ammonium sulfate 10g / L, yeast extract 20g / L, potassium chloride 1.5g / L, NaCl 0.1g / L, pH4.0) In shake flasks, cultured on a shaker at 110 rpm at 32°C for 24 hours. Then transfer 10mL to 100mL liquid fermentation medium (molasses 45g / L, (NH 4 ) 2 SO 4 3g / L,H 3 PO 4 0.7ml / L, MgSO 4 0.5g / L, FeSO 4 0.05g / L, the pH of the medium was adjusted to 4.0 with ammonia water) in a 500mL shaker flask...

Embodiment 2

[0033] Transfer the strain TKZY-06 from the wort slant to a ring containing 30mL liquid seed medium (molasses 10g / L, ammonium sulfate 5g / L, yeast extract 10g / L, potassium chloride 0.1g / L, NaCl0.5g / L, pH 4.0) in a 500mL shaker flask, cultured on a 110rpm shaker at 33°C for 24 hours. Transfer 8mL to 100mL liquid fermentation medium (molasses 50g / L, (NH 4 ) 2 SO 4 1.5g / L,H 3 PO 4 0.8ml / L, MgSO 4 0.2g / L, FeSO 4 2.0g / L, the pH of the medium was adjusted to 5.0 with ammonia water) in a 500mL shake flask, and fermented on a shaking table at 240rpm at 30°C for 16 hours. The RNA content of the bacteria can reach 17.5% of the dry weight of the bacteria.

Embodiment 3

[0035] Transfer the bacterial strain TKZY-06 from the wort slant to a ring containing 30mL liquid seed medium (glucose 25g / L, ammonium sulfate 15g / L, corn steep liquor 30g / L, potassium chloride 6g / L, NaCl8g / L, pH 6.0) in a 500mL shaker flask, cultured on a shaker at 110rpm at 31°C for 24 hours. Transfer 10mL to 100mL liquid fermentation medium (maltose 50g / L, (NH 4 ) 2 SO 4 2g / L, H 3 PO 41ml / L, MgSO 4 0.8g / L, FeSO 4 1.6g / L, the pH of the medium was adjusted to 5.0 with ammonia water) in a 500mL shaker flask, and fermented at 240rpm at 32°C for 17 hours. The RNA content of the bacteria can reach 15% of the dry weight of the bacteria.

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Abstract

The invention discloses saccharomyces cerevisiae for producing ribonucleic acid by fermentation. The saccharomyces cerevisiae has a strain number of TKZZY-06 and is preserved in China General Microbiological Culture Collection Center, China Committee for Culture Collection of Microorganisms, on April 26th, 2013, with a preservation number of CGMCC No. 7522. The invention also discloses an application of the saccharomyces cerevisiae. RNA content of the TKZY-06 strain thallus can reach 17.5% that of dry weight of the thallus and is increased by 9.5% than RNA yield of an original strain. The strain is subcultured by over 10 generations; and the performance for producing the ribonucleic acid can be kept stable.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a Saccharomyces cerevisiae with high yield of ribonucleic acid and an application thereof. Background technique [0002] Ribonucleic acid (RNA for short) is a very important class of biological macromolecules. RNA not only plays a key role in gene expression and protein biosynthesis, but also promotes many physiological functions of cells. With the deepening of research, the application prospects of ribonucleic acid in medicine, health care, agriculture, food processing and other industries are becoming wider and wider. In medicine, RNA is an important pharmaceutical intermediate, and the nucleotides, nucleosides and bases obtained after its degradation are all widely used drugs, and their derivatives have more significant applications in antitumor and antiviral aspects. Scientific research has proved that the aging of the human body is closely related to the ability to sy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P19/34C12R1/865
Inventor 陈晓春黄小权蔡家栋张磊盛兵汤亦文陈勇
Owner NANJING BIOTOGETHER
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