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Preparation method of multi-template molecular imprinting integrated bar

A technology of molecular imprinting and template molecules, applied in chemical instruments and methods, alkali metal oxides/hydroxides, inorganic chemistry, etc., to achieve the effects of easy regulation, suitable thickness and simple preparation

Inactive Publication Date: 2014-05-07
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are very few reports on the use of two or more compounds as template molecules, and there is no synthesis of molecularly imprinted polymers using multiple compounds of sulfonamide antibiotics and antibacterial synergists as template molecules.

Method used

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  • Preparation method of multi-template molecular imprinting integrated bar
  • Preparation method of multi-template molecular imprinting integrated bar
  • Preparation method of multi-template molecular imprinting integrated bar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The preparation method of the multi-template molecularly imprinted monolith described in this embodiment comprises the following steps:

[0030] (1) Clean the hard glass capillary with an inner diameter of 0.5mm, dry it at 150°C, and sinter one end to serve as a reaction vessel for molecularly imprinted polymer preparation;

[0031] (2) Dissolve 0.33 mmol of sulfamethazine, 0.33 mmol of sulfamethoxazole, and 0.33 mmol of trimethoprim in 10 mL of acetonitrile, add 4 mmol of methacrylic acid, mix well, and place in the dark for 12 h. Obtain the pre-assembly solution;

[0032] (3) Add 18 mmol of trimethylolpropane trimethacrylate and 0.5 mmol of azobisisobutyronitrile to the pre-assembly solution in step (2), mix well, degas by ultrasonic for 5 min, and inject with a syringe with an inner diameter of 0.5 mm rigid capillary, heated in a water bath at 45°C to initiate polymerization;

[0033] (4) After polymerization for 20 h, soak overnight in a 1:6 water-hydrofluoric aci...

Embodiment 2

[0038] The preparation method of the multi-template molecularly imprinted monolith described in this embodiment comprises the following steps:

[0039] (1) Clean the hard glass capillary with an inner diameter of 1.0 mm, dry it at 150°C, and sinter one end to serve as a reaction vessel for molecularly imprinted polymer preparation;

[0040] (2) Dissolve 0.66 mmol of sulfamethazine, 0.66 mmol of sulfamethoxazole, and 0.66 mmol of trimethoprim in 10 mL of acetonitrile, add 8 mmol of methacrylic acid, mix well, and place in the dark for 16 h. Obtain the pre-assembly solution;

[0041] (3) Add 18 mmol of trimethylolpropane trimethacrylate and 1 mmol of azobisisobutyronitrile to the pre-assembly solution in step (2), mix well, ultrasonically degas for 5 minutes, and inject with a syringe with an inner diameter of 1.5 mm The rigid capillary tube is heated in a water bath at 75°C to initiate polymerization;

[0042] (4) After polymerization for 28 hours, soak overnight in a 1:2 wat...

Embodiment 3

[0047] The preparation method of the multi-template molecularly imprinted monolith described in this embodiment, such as figure 1 shown, including the following steps:

[0048] (1) Wash the hard glass capillary with an inner diameter of 1.5 mm, dry it at 150 °C, and sinter one end to serve as a reaction vessel for molecularly imprinted polymer preparation;

[0049] (2) Dissolve 0.33 mmol of sulfamethazine, 0.33 mmol of sulfamethoxazole and 0.50 mmol of trimethoprim in 10 mL of acetonitrile as template molecules, add 4 mmol of methacrylic acid, mix well, and place in the dark for 14 h to obtain pre-assembly solution;

[0050] (3) Add 18mmol trimethylolpropane trimethacrylate and 0.75mmol azobisisobutyronitrile to the pre-assembly solution in step (2), mix well, ultrasonically degas for 5 min, and inject with a syringe with an inner diameter of 1.0 mm hard capillary, heated in a water bath at 60°C to initiate polymerization;

[0051] (4) After 24 hours of polymerization, soak...

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Abstract

The invention discloses a preparation method of a multi-template molecular imprinting integrated bar, and belongs to the technical fields of preparation of polymer materials and pretreatment of samples. The method comprises the following steps of: getting a hard glass capillary as a reaction vessel of a molecular imprinting polymer integrated bar; cleaning the capillary; drying; sintering one end; adding a certain amount of molecular imprinting pre-assembling solution; heating with water bath to initiate polymerization; removing the capillary and ageing after polymerization; capturing with a proper length; eluting template molecule to obtain multi-template molecular imprinting integrated bar for extracting and analyzing the samples. The prepared multi-template molecular imprinting integrated bar is able to selectively separate and enrich sulfanilamide antibiotics and antibacterial synergist compounds at the same time, and is applicable to multi-residue synchronous analysis of the sulfanilamide antibiotics and antibacterial synergists in the food and environmental samples.

Description

technical field [0001] The invention relates to a method for preparing a multi-template molecularly imprinted integral rod, which belongs to the technical field of polymer material preparation and sample pretreatment. Background technique [0002] Molecular imprinting technology originated in immunology, and experienced antigen-antibody theory, clonal selection theory and specific adsorption theory, but the real attention and attention began with the report on theophylline molecularly imprinted polymer in Nature in 1993 ( Vlatakis G, Andersson L I, Muller R, Mosbach K. Nature, 1993, 361(6413): 645-647). Molecularly imprinted polymers have good specific recognition ability and can resist the interference of complex matrices, and are widely used in sample pretreatment, chromatographic stationary phase, biomimetic sensing, simulated enzyme catalysis and other fields. [0003] The most commonly used method for the preparation of molecularly imprinted polymers (Molecularly Impri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/20C08F220/06C08J9/26B01J20/26B01J20/28B01J20/30
Inventor 许志刚陈云丽刘智敏王联广杨保民字富庭
Owner KUNMING UNIV OF SCI & TECH
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