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Method for preparing glucose from bamboo cellulose

A technology of bamboo cellulose and glucose, applied in the direction of fermentation, etc., can solve the problems of difficult control and complicated equipment, and achieve the effects of non-combustibility and explosion, improved hydrolysis conversion rate, and easy control of the production process

Inactive Publication Date: 2014-04-30
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese invention patent CN101798584A adopts the continuous hydrothermal method to improve the enzymatic hydrolysis and conversion of cellulose to produce ethanol. The processing temperature is 170-230°C. The equipment used is relatively complicated and difficult to control.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Take bamboo powder with a particle size of 20-80 mesh, and determine its chemical composition according to the method of the National Renewable Energy Laboratory (NREL, TP-510-42618). The main components of bamboo powder are 43.44% cellulose, 29.56% hemicellulose and 27.53% lignin. Mix 20g of bamboo powder with 200g of water, put it into an intermittent pressure-resistant reactor, seal it and start stirring at a stirring speed of 150 rpm, use an electric furnace to heat the reactor, and heat it from room temperature at a heating rate of 5°C / min to 220°C, then rapidly cooled to 80°C with condensed water at a cooling rate of 23°C / min, and then naturally cooled to room temperature to obtain a reaction mixture;

[0019] (2) Filter the above reaction mixture, wash the filtered residue with water at 20° C., and air-dry the residue at room temperature for 48 hours to obtain a hydrothermally treated product. The hydrothermal treatment sample yield was 66.43%, and the main ...

Embodiment 2

[0021] (1) Take bamboo powder with a particle size of 20-80 mesh, and determine its chemical composition according to the method of the National Renewable Energy Laboratory (NREL, TP-510-42618). The main components of bamboo powder are 43.44% cellulose, 29.56% hemicellulose and 27.53% lignin. Mix 20g of bamboo powder with 200g of water, put it into an intermittent pressure-resistant reactor, seal it and start stirring at a stirring speed of 150 rpm, use an electric furnace to heat the reactor, and heat it from room temperature at a heating rate of 5°C / min to 220°C, then rapidly cooled to 80°C with condensed water at a cooling rate of 23°C / min, and then naturally cooled to room temperature to obtain a reaction mixture;

[0022] (2) Filter the above reaction mixture, wash the filtered residue with water at 20° C., and air-dry the residue at room temperature for 48 hours to obtain a hydrothermally treated product. The hydrothermal treatment sample yield was 66.43%, and the main ...

Embodiment 3

[0024] (1) Take bamboo powder with a particle size of 20-80 mesh, and determine its chemical composition according to the method of the National Renewable Energy Laboratory (NREL, TP-510-42618). The main components of bamboo powder are 43.44% cellulose, 29.56% hemicellulose and 27.53% lignin. Mix 20g of bamboo powder with 200g of water, put it into an intermittent pressure-resistant reactor, seal it and start stirring at a stirring speed of 150 rpm, use an electric furnace to heat the reactor, and heat it from room temperature at a heating rate of 5°C / min to 240° C., then rapidly cooled to 80° C. with condensed water at a cooling rate of 23° C. / min, and then naturally cooled to room temperature to obtain a reaction mixture;

[0025] (2) Filter the above reaction mixture, wash the filtered residue with water at 20° C., and air-dry the residue at room temperature for 48 hours to obtain a hydrothermally treated product. The hydrothermal treatment sample yield was 55.91%, the mai...

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Abstract

The invention relates to a method for preparing glucose from bamboo cellulose, belongs to the technical field of utiliztion of lignocellulose. Bamboo used as the raw material is subjected to hydrothermal treatment by a batch reactor, wherein the heating rate, maximum reaction temperature (250-240 DEG C) and cooling rate of the reaction are controlled to implement pretreatment on the bamboo cellulose; and the pretreated sample is subjected to enzyme hydrolysis to obtain the glucose. The method is simple and environment-friendly in the process and easy to control, and has favorable application prospects. After the bamboo cellulose is pretreated, the conversion rate of enzyme hydrolysis for generating glucose can be enhanced by 3.7 times, thereby implementing high-efficiency utilization of bamboo resources.

Description

technical field [0001] The invention relates to a method for preparing glucose by using bamboo cellulose, which belongs to the technical field of lignocellulose pretreatment. technical background [0002] As the shortage of fossil resources becomes increasingly prominent, more and more attention has been paid to the production of energy from lignocellulose. Lignocellulose is mainly composed of cellulose, hemicellulose and lignin with some other minor components. Bamboo is an important lignocellulose resource, and the development of bamboo to prepare biofuels is of great significance to ensure my country's energy security. [0003] In the process of preparing bioethanol or biobutanol, it is necessary to prepare glucose that can be used for fermentation. The cellulose, hemicellulose, and lignin of lignocellulose form a heterogeneous structure through chemical bonds and special forces, which hinder the degradation of each component. To overcome the anti-degradation barrier o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02
Inventor 李明飞边静陈昌洲孙润仓肖霄
Owner BEIJING FORESTRY UNIVERSITY
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