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Acremonium terricola mutant strain and application thereof

A technique of acremonium acremonium and mutagenic strains, applied in the field of microbial germplasm resource utilization, can solve the problems of low content of secondary metabolite adenosine and limit the utilization of acremonium acremonium, so as to improve production performance and health The effect of the situation

Active Publication Date: 2014-04-30
合肥迈可罗生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the content of secondary metabolites adenosine, sterols, crude protein and polysaccharides in the currently discovered strains of Acremonium acremonium is not high, which limits the further utilization of Acremonium acremonium

Method used

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  • Acremonium terricola mutant strain and application thereof
  • Acremonium terricola mutant strain and application thereof
  • Acremonium terricola mutant strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 mutagenesis screening obtains acremonium acremonium mutagenic strain

[0036] 1 Departure strain isolation

[0037] (1) Cordyceps collection

[0038] Cordyceps is collected from Sangongjian, Wenquan Town, Yuexi County, Anhui Province, with fresh subsea. It was identified as a Cordyceps gunnii var.minor.

[0039] (2) Isolation, purification and identification of Cordyceps fungus

[0040] Under aseptic conditions, rinse the fresh Cordyceps cells thoroughly with sterile water, then soak them in 0.5% chloramine T for 15 minutes, and then rinse thoroughly with sterile water; carefully cut the Cordyceps cells longitudinally with a sterile scalpel Divide into 4 petals and place them on 4 plates covered with SDAY medium; culture at 20°C for about two weeks until colonies visible to the naked eye grow; use an inoculation needle to pick out the mycelia of the colonies and transfer them to another fresh SADY plate, 20 Cultivate at ℃ for about a week to grow a puri...

Embodiment 2

[0098] Example 2 Preparation of Feed Additives Using Acremonium terricola Mutant Strain (Acremonium terricola MKL18)

[0099] 1 Preparation of seed solution

[0100] Take the mutant strain of Acremonium terricola (Acremonium terricola MKL18) preserved at 4°C, inoculate it on a PDA slant, and culture it at 26°C for 7 days; the colonies on the slant are transferred to a 1000ml Erlenmeyer flask containing 200ml of liquid Chapeauer's medium, 26 Cultivate with shaking at 125r / min for 4 days; transfer the culture solution once again according to the inoculum size of 10%, and culture with shaking under the same conditions for 4 to 5 days to obtain the seed solution.

[0101] 2 liquid fermentation

[0102] In a 50L air-lift fermenter, add 30L of fermentation culture medium, and add appropriate amount of foam enemy, heat and sterilize at 115°C for 20 minutes; when the temperature drops to 26°C, add 600ml of seed liquid; keep the temperature in the tank at 26°C, and ventilate Amount o...

Embodiment 3

[0119] The influence of embodiment 3 feed additives on growth performance and health status of piglets

[0120] Select 200 healthy Du-Chang-Da Sanyuan hybrid piglets with similar age and weight (7.95±0.87kg) at 30 days, half male and half female, and randomly divided into 4 groups according to the principle of similar size and sex, 50 pigs in each group;

[0121] The feed additive (solid matrix of formula 2) prepared by fermentation of the mutagenic strain in Example 2 was added to the basal ration at a ratio of 0.25%, 0.5%, and 0.75%, respectively, and fed to the three groups of piglets respectively. group; one group of piglets fed directly with the basal diet served as the control group. Observe and record the growth performance and health status of the piglets in the test group and the control group, and the results are shown in Table 5 and Table 6, respectively.

[0122] Table 5 Effects of feed additives on growth performance of piglets

[0123]

[0124] Note: a and b...

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Abstract

The invention discloses an acremonium terricola mutant strain and an application thereof. The collection number of the acremonium terricola mutant strain (MKL18) is CCTCC No.M2013654; and the application refers to the application thereof in preparing feed additives, drugs and healthcare products. In the invention, the acremonium terricola MKL18 is obtained through ultraviolet mutation screening, the mycelium extract thereof has in-vitro anti-tumor activity and a function of enhancing the in-vivo immunocompetence of an animal and can be used for preparing drugs and healthcare food; the acremonium terricola MKL18 is subjected to liquid-solid two-phase fermentation, and the fermentation product is dried and ground and then can be directly used as a feed additive; and the feed additive remarkably improves the productivity of weaned piglets and remarkably improves the health state of the weaned piglets.

Description

technical field [0001] The invention belongs to the technical field of utilization of microbial germplasm resources, and in particular relates to a mutagenic strain of Acremonium acremonium and its application. Background technique [0002] Cordyceps is a biological complex formed by fungi infecting insect larvae. Currently, there are more than 350 species of Cordyceps found in the world, and more than 70 species have been found in China alone. Among them, the most famous Cordyceps sinensis has long been regarded as a precious traditional Chinese medicine, and scientific research has also confirmed that Cordyceps sinensis contains a variety of pharmacologically active ingredients. [0003] Among the secondary metabolites of Cordyceps and related fungi, components such as adenosine, sterols, and polysaccharides are related to many pharmacological activities of Cordyceps. Among them, adenosine has a variety of biological activities, including sedative, blood vessel expansion ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A23K1/16A61K36/068A61P35/00A61P37/04A23L1/29C12R1/645A23L33/00
Inventor 李晓祥桂向东李德刚徐自奥
Owner 合肥迈可罗生物工程有限公司
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